國立陽明大學 生命科學院

生物藥學研究所、生命科學系暨基因體科學研究所

 

林照雄教授

醫大樓甲棟一樓112 chlin2@ym.edu.tw

研究領域:蛋白質體學、質譜儀分析、化學生物學

研究方向:

探討癌細胞中之hnRNPK蛋白質,透過精氨酸甲基化抑制細胞凋亡之調控機制

探討抗癌天然物穿心蓮內酯andrographolide抑制黑色素瘤細胞之惡性轉移的代謝機制

利用酵素探針策略以篩選可抑制癌細胞生長或轉移的代謝途徑

發展系統化鑑定蛋白質轉譯後修飾之策略及後續功能性研究

 

研究發表:

2011-2014

Jen-Hao Yang, Yi-Ying Chiou, Shu-Ling Fu, I-Yun Shih, Tsai-Hsuan Weng, Wey-Jinq Lin, Chao-Hsiung Lin* 2014, Aug. "Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation" Nucleic Acids Research, 9908-9924. (SCI) NSC101-2311-B-010-006, 101-2321-B-010-016 and 102-2321-B-010-014

Chih-Hao Wang, Yi-Fan Chen, Chia-Yu Wu, Pei-Chun Wu, Yi-Long Huang, Cheng-Heng Kao, Chao-Hsiung Lin, Lung-Sen Kao, Ting-Fen Tsai*, and Yau-Huei Wei* 2014, May "Cisd2 modulates the differentiation and functioning of adipocytes by regulating intracellular Ca2+ homeostasis" Human Molecular Genetics, 4770-4785.

Min-Han Lin, Shang-Ju Chuang, Chiao-Che Chen, Shu-Chun Cheng, Kai-Wen Cheng, Chao-Hsiung Lin, Chiao-Yin Sun*, and Chi-Yuan Chou* 2014, Jun. " Structural and functional characterization of MERS coronavirus papain-like protease" Journal of Biomedical Science, 54. (SCI)

Yan-Su Lin, Yi-Long Huang, Wen-Fu Lee*, and Chao-Hsiung Lin* 2014, May "Property and Application of BACy-Based Functional Hydrogels" Journal of the Chinese Chemical Society, 945-952. (SCI) NSC97-2113-M-010-001, 101-2321-B-010-016 and 101-2311-B-010-006  

Yan-Su Lin, Chao-Hsiung Lin, and Wen-Fu Lee* 2014, Jan. "Investigation of the synthesis and properties of the copolymeric hydrogels based on N-isopropyl acrylamide and acrylamidoazobenzene" Journal of Polymer Research, 364. (SCI)

Sheng-Hung Liu, Chao-Hsiung Lin, Fong-Ping Liang, Pei-Fen Chen, Cheng-Deng Kuo, Mohd. Mujahid Alam, Barnali Maiti, Shih-Kai Hung, Chin-Wen Chi, Chung-Ming Sun* and Shu-Ling Fu* 2014, Jan. "Andrographolide downregulates the v-Src and Bcr-Abl oncoproteins and induces Hsp90 cleavage in the ROS-dependent suppression of cancer malignancy" Biochemical Pharmacology, 229-242. (SCI)

Fong-Lee Huang, Young-Ji Shiao, Sheue-Jane Hou, Cheng-Ning Yang, Yi-Jen Chen, Chao-Hsiung Lin, Feng-Shiun Shie and Huey-Jen Tsay* 2013, Aug. "Cysteine-rich domain of scavenger receptor AI modulates the efficacy of surface targeting and mediates oligomeric Aβ internalization" Journal of Biomedical Science, 54. (SCI)

Sheng-Hui Tsai, Gwan-Han Shen, Chao-Hsiung Lin, Jiue-Ru Liau, Hsin-Chih Lai, and Shiau-Ting Hu* 2013, Jun. "Mab_3168c, a Putative Acetyltransferase, Enhances Adherence, Intracellular Survival and Antimicrobial Resistance of Mycobacterium abscessus" PLoS ONE, e67563. (SCI)

Yong-Shiang Lin, Chao-Hsiung Lin, Li-De Huang, Tung Chao, Cheng-Deng Kuo, Ling-Chien Hung, Fen-Hwa Wong, Chun-Cheng Lin and Shu-Ling Fu* 2013, Jul. "The suppression of thoc1 in cancer cell apoptosis mediated by activated macrophages is nitric oxide-dependent" Biochemical Pharmacology, 242-252. (SCI)

Kai-Wei Hsueh, Shu-Ling Fu, Chirn-Bin Chang, Yu-Ling Chang and Chao-Hsiung Lin* 2013, Feb. "A novel Aurora-A-mediated phosphorylation of p53 inhibits its interaction with MDM2" Biochimica et Biophysica Acta - Proteins and Proteomics, 508-515. (SCI) NSC98-2311-B-010-003-MY3 and VGHUST100-G7-5-1

Yan-Su Lin, Hsuan-Hsuan Lee, Wen-Fu Lee and Chao-Hsiung Lin* 2013, Feb. "Synthesis and qualitative analysis of BACy and its self-polymer" Journal of the Chinese Chemical Society, 223-228. (SCI) NSC97-2113-M-010-001 and NSC98-2311-B-010-003-MY3

Yi-Long Huang, Tsai-Wen Chung, Chia-Mao Chang, Chih-Hau Chen, Chen-Chung Liao, Yeou-Guang Tsay, Gwo-Chyuan Shaw, Shwu-Huey Liaw, Chung-Ming Sun* and Chao-Hsiung Lin* 2012, Nov. "Qualitative analysis of the fluorophosphonate-based chemical probes targeting recombinant and endogenous serine hydrolase" Analytical and Bioanalytical Chemistry, 2387-2396. (SCI) NSC97-2113-M-010-001 and NSC98-2311-B-010-003-MY3

Chih-Liang Tien, Chao-Hsiung Lin, Ting-Hui Lin, Feng-Chen Wen, Chien-Wei Su, Sheng-Seen Fan and Mingli Hsieh* 2012, Sep. "Secreted cyclophilin A induction during embryo implantation in a model of human trophoblast-endometrial epithelium interaction" European Journal of Obstetrics & Gynecology and Reproductive Biology, 55-59. (SCI)

Yao-Ping Lin, Chih-Yu Yang, Chen-Chung Liao, Wen-Chung Yu, Chin-Wen Chi and Chao-Hsiung Lin* 2012, Jul. "Plasma protein characteristics of long-term hemodialysis survivors" PLoS ONE, e40232. (SCI) NSC96-2317-B-010-001, NSC98-2311-B-010-003-MY3 and VGHUST100-G7-5-1

Yi-Chang Liu#, I-Hsuan Lin#, Wei-Jen Chung#, Wensi S. Hu, Wailap Victor Ng, Chi-Yu Lu, Tsung-Yen Huang, Hung-Wei Shu, Kwang-Jen Hsiao, Shih-Feng Tsai, Chuan-Hsiung Chang* and Chao-Hsiung Lin* 2012, Mar. "Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen Mycoplasma fermentans M64" PLoS ONE, e35304. (SCI) NSC98-2311-B-010-003-MY3

Yi-Feng Huang, Jing-Jer Lin, Chao-Hsiung Lin, Yeu Su*, Shih-Chieh Hung* 2012, Jan. "c-Jun N-terminal kinase 1 negatively regulates osteoblastic differentiation induced by BMP-2 via phosphorylation of Runx2 at Ser104" Journal of Bone and Mineral Research, 1093-1105. (SCI)

Yi-Ying Chiou, Shu-Ling Fu, Wey-Jinq Lin, and Chao-Hsiung Lin* 2012, Feb. "Proteomics analysis of in vitro protein methylation during Src-induced transformation" Electrophoresis 451-461. (SCI) NSC 98-2311-B-010-003-MY3

Yong-Shiang Lin, Li-Der Huang, Chao-Hsiung Lin, Po-Hsiung Huang, Yu-Jen Chen, Fen-Hwa Wong, Chun-Cheng Lin*, and Shu-Ling Fu* 2011, Dec. "In vitro and in vivo anticancer activity of a synthetic glycolipid as TLR4 activator" J Biol Chem 43782-43792. (SCI)

Kai-Wei Hsueh, Shu-Ling Fu, Chi-Ying Huang and Chao-Hsiung Lin* 2011, Aug. "Aurora-A Phosphorylates HnRNPK and Disrupts Its Interaction With p53" FEBS Lett 2671-2675. (SCI) NSC 98-2311-B-010-003-MY3

 

2003-2010

YuanI Chang, ShengWei Lin, YiYing Chiou, JungSung Sung, LeeChun Cheng, YuLing Lu, KuangHui Sun, Keejong Chang, ChaoHsiung Lin, and WeyJinq Lin* 2010, Dec. "Establishment of an ectopically expressed and functional PRMT1 for proteomic analysis of arginine-methylated proteins" Electrophoresis, 3834-3842. (SCI) 

Sheng-Hung Liu, Chao-Hsiung Lin, Jen-Hwey Chiu, Shih-Kai Hung, Chin-Wen Chi, and Shu-ling Fu* 2010, Oct. "Fisetin Inhibits Macrophage Activation and Dendritic Cell Maturation" Journal of Agricultural and Food Chemistry, 10831-10839. (SCI) 

Yao-Ping Lin, Meng-Erh Hsu, Yi-Ying Chiou, Hung-Yi Hsu, Hiao-Chien Tsai, Yu-Ju Peng, Chi-Yu Lu, Chien-Yuan Pan, Wen-Chung Yu, Chen-Huan Chen, Chin-Wen Chi, and Chao-Hsiung Lin* 2010, Jul. "Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model" Proteomics, 2429-2443. (SCI) NSC 95–2627-M-007–002, NSC 97–2317-B-010–001

Chao-Hsiung Lin, Hsaing-Yin Chen, Chih-Jen Yu, Pen-Li Lu, Chu-Hsin Hsieh, Bao-Yu Hsieh, Ying-Feng Chang, and Chien Chou* 2009, Feb. "Quantitative measurement of binding kinetics in sandwich assay using a fluorescence detection fiber-optic biosensor" Analytical Biochemistry, 224-228. (SCI) NSC 95-2323-B-010-001

Jack C. Yu*, Jung-Ren Chen, Chao-Hsiung Lin, Guigen Zhang, Poh-Sang Lam, Karl H. Wenger, Farid B. Mozaffari, Shun-Te Huang, James L. Borke 2009, Feb. "Tensile Strain-Induced Ets-2 Phosphorylation by CaMK II and the Homeostasis of Cranial Sutures" Plastic and Reconstructive Surgery, 83s-93s. (SCI) 

Yi-Fang Wu, Shu-Ling Fu, Cheng-Heng Kao, Chu-Wen Yang, Chao-Hsiung Lin, Ming-Ta Hsu and Ting-Fen Tsai* 2008, Mar. "Chemopreventive Effect of Silymarin on Liver Pathology in HBV X Protein Transgenic Mice" Cancer Research, 2033-2042. (SCI) 

Choun-Sea Lin, Nien-Tzu Liu, De-Chih Liao, Jau-Song Yu, Chan-Hwei Tsao, Chao-Hsiung Lin, Chih-Wen Sung, Wann-Neng Jane, Hsing Sheng Tsay, Jeremy Jain-Wei Chen, Na-Shen Lin, Wei-Chin Chang, Chung-Chih Lin* 2008, Mar. "Differential protein expression of two photosystem II subunits, PsbO and PsbP in an albino mutant of Bambusa edulis Murno with chloroplast DNA aberration" Journal of the American Society for Horticultural Science, 270-277. (SCI) 

Cheng-Jen, Liao, Ko-Hsin Chin, Chao-Hsiung Lin, Shi-Fong Tsai, Ping-Jiang Lyu, Chiu-Chong Young, Andrew H.-J. Wang, & Shan-Ho Chou* 2008, Nov. "Crystal Structure of DFA0005 Complexed with α-Ketoglutarate: A Novel Member of the ICL/PEPM Superfamily from Alkali-tolerant Deinococcus ficus" Proteins: Structure, Function and Bioinformatics, 362-371. (SCI) 

Fong-Pin Liang, Chao-Hsiung Lin, Cheng-Deng Kuo, Hsueh-Ping Chao, and Shu-Ling Fu* 2008, Feb. "Suppression of v-SRC transformation by andrographolide via degradation of the v-SRC protein and attenuation of the ERK-signaling pathway" Journal of Biological Chemistry 5023-5033. (SCI) NSC 96-2317-B-010-001

Hui-Chuan Chang, Liang-Yu Chen, Yi-Hang Lu, Meng-Ying Li, Yu-Hou Chen, Chao-Hsiung Lin, and Gu-Gang Chang* 2007, Dec. "Metal ions Stabilize a Dimeric Molten Globule State Between the Open- and Closed-Forms of Malic Enzyme" Biophysical journal 3977-3988. (SCI) 

Chi-Yu Lu, Chiu-Yi Wu, and Chao-Hsiung Lin* 2007, Aug. "Protein identification by a syringe pump-driven reversed-phase LC-MS/MS" Analytical Biochemistry 123-129. (SCI) NSC 94-2627-M-007-002, NSC 94-2120-M-010-002  

Wei-Jen Chung, Hung-Yu Shu, Chi-Yu Lu, Chiu-Yi Wu, Yi-Hsiung Tseng, Shih-Feng Tsai, and Chao-Hsiung Lin* 2007, Aug. "Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17" Proteomics 2047-2058. (SCI) NSC 91-B-FA05-1-4, 95-2120-M-010-002, 95-2627-M-007-002

Bao-Yu Hsieh, Ying-Feng Chang, Ming-Yaw Ng, Wei-Chih Liu, Chao-Hsiung Lin, Hsieh-Ting Wu, and Chien Chou* 2007, May "Localized Surface Plasmon Coupled Fluorescence Fiber-Optic Biosensor with Gold Nanoparticles" Analytical Chemistry 3487-3493. (SCI)

Yi-Ying Chiou, Wey-Jinq Lin, Shu-Ling Fu, and Chao-Hsiung Lin* 2007, Feb. "Direct mass- spectrometric identification of Arg296 and Arg299 as the methylation sites of hnRNP K protein for methyltransferase PRMT1" Protein Journal 87-93. (SCI) NSC 94-2120-M-010-002, 94-2627-M-007-002

Manohar V. Kulkarni, Geeta M. Kulkarni, Chao-Hsiung Lin and Chung Ming Sun* 2006, Oct. "Recent advances in coumarins and 1-azacoumarins as versatile biodynamic agents" Current Medicinal Chemistry 2795-2818 (SCI).

Shen-Chia Chen, Yuan-Chih Chang, Chao-Hsiung Lin, Chun-Hung Lin and Shwu-Huey Liaw* 2006, Nov. "Crystal structure of a bifunctional deaminase and reductase from bacillus subtilis involved in riboflavin biosynthesis" J. Biol. Chem. 7605-7613 (SCI).

Chung-Shu Lee, Ching-Te Chien, Chao-Hsiung Lin, Yi-Ying Chiu and Yuh-Shyong Yang* 2006, Jul.  "Protein changes between dormant and dormancy-broken seed of Prunus campanulata Maxim" Proteomics 4147-4154 (SCI).

Shu-Ling Fu*, Ya-Hui Hsu, Pei-Yeh Lee, Wen-Chi Hou, Ling-Chien Hung, Chao-Hsiung Lin, Chiu-Ming Chen, and Yu-Jing Huan 2006, Jan. "Dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages" Biochem. Biophys. Res. Commun. 137-144 (SCI).

Chi-Yuan Chou, Hui-Chuan Chang, Wen-Chi Hsu, Tien-Zheng Lin, Chao-Hsiung Lin, and Gu-Gang Chang* 2004, Nov. "Quaternary Structure of the Severe Acute Respiratory Syndrome (SARS) Coronavirus Main Protease" Biochemistry 14958-14970 (SCI).

Meng-Ting Tsou, Jin-Yuan Ho, Chao-Hsiung Lin, and Jen-Hwey Chiu* 2004, Oct. "Proteomic analysis finds different myocardial protective mechanisms for median nerve stimulation by electroacupuncture and by local somatothermal stimulation" Int. J. Mol. Med. 553-563 (SCI).

Chung-Pin Li, Jen-Hsin Huang, Ai-Chi Chang, Yi-Mei Hung, Chao-Hsiung Lin, Yee Chao, Shou-Dong Lee, Jacqueline Whang-Peng, and Tse-Sing Huang* 2004, Jan. "A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cells " Pharm. Res. 93-100 (SCI).

Feng-Chen Wen, Yen-Hsien Li, Hui-Fang Tsai, Chao-Hsiung Lin, Chuan Li, Chin-San Liu, Chong-Kuei Lii, Nobuyuki Nukina and Mingli Hsieh* 2003, Jul. "Down-regulation of heat shock protein 27 in neuronal cells and non-neuronal cells expressing mutant ataxin-3" FEBS Lett. 307-314 (SCI).