洪善鈴 教授(Hung, Shan-Ling, PhD)

個人簡歷

學歷PhD, Molecular Biology Graduate Group, Program in Microbiology, University of Pennsylvania, PA, USA (美國賓系法尼亞大學哲學博士)

專長病毒學、細菌學、分子生物學

聯絡電話(02) 2826-7224

傳真電話(02) 2826-4053

電子郵件slhung@ym.edu.tw

 

研究方向

(一)單純皰疹病毒的致病機轉:單純皰疹病毒(Herpes simplex virus)可引起原發性及潛在性感染,第一型(HSV-1)主要引起口腔感染,而第二型(HSV-2)主要為生殖道皰疹。本研究室主要利用病毒學、免疫學、細胞及分子生物學等技術,探討單純皰疹病毒的致病機轉,包括:(1) 病毒感染口腔細胞之機轉,感染細胞過程中病毒蛋白及細胞接合器之交互作用,及對訊息傳遞的影響;及(2)單純皰疹病毒與牙周病及牙周致病菌之間可能的關係。

(二) 檳榔嚼食對微生物及免疫的影響:嚼食檳榔與口腔鱗狀上皮細胞癌(oral squamous cell carcinoma)和口腔黏膜下纖維化(oral submucous fibrosis)具 有很高的相關性。本研究室主要探討:檳榔對口腔微生物及口腔組織的影響,比較檳榔嚼食與否,對牙周健康的影響,利用微生物及分子生物學的方法,了解檳榔對 口腔微生物族群的影響;探討嚼食檳榔對免疫系統可能的影響,分析檳榔嚼塊成份對嗜中性白血球及單核球細胞功能之影響,並探討免疫調控基因之表現及功能與 嚼食相關之口腔癌化過程之間的相關性,進一步了解檳榔引起口腔癌化相關之免疫基因的改變及可能參與的危險因子及分子機轉。

研究計畫(五年內)

年度

計畫名稱 

類型

計畫內擔任之工作

執行期間 

經費提供單位 

2018

口腔上皮表現第一型穿膜黏蛋白與嗜中性球功能的交互作用

個人

主持人

2018/01/01 ~ 2018/12/31

台北市政府衛生局

2017

穿膜蛋白對單純皰疹病毒第一型感染口腔上皮之影響

個人

主持人

2017/01/01 ~ 2017/12/31

台北市政府衛生局

2016

表沒食子兒茶素沒食子酸酯對於單純皰疹病毒與口腔致病菌感染的影響

個人

主持人

2016/08/01 ~ 2018/07/31

科技部

2015

單純皰疹病毒與口腔致病菌及口腔細胞的交互作用

個人

主持人

2015/08/01 ~ 2016/07/31

科技部

2015

穿膜黏蛋白對牙周致病菌感染口腔上皮之影響

個人

主持人

2015/01/01 ~ 2015/12/31

台北市政府衛生局

2014

綠茶多代田菌(養樂多益生菌)抑制齲齒形成機轉的影響

個人

主持人

2014/01/01 ~ 2014/12/31

台北市政府衛生局

2013

益生菌G菌對感染牙周致病菌Porphyromonas gingivalis之人類齒齦上皮細胞之影響

個人

主持人

2013/01/01 ~ 2013/12/31

台北市政府衛生局

2012

單純皰疹病毒感染對人類口腔上皮細胞蛋白激酶Akt下游受質的影響及調控機轉

個人

主持人

2012/08/01 ~ 2015/07/31

科技部

期刊論文(* 通訊作者)

1.     Chang C-W, Lin H-H, Wu S-Y, Wu C-Y, Lai Y-L, Hung S-L*. Association between monocyte chemoattractant protein-1 -2518 A/G gene polymorphism and the outcome of the nonsurgical periodontal treatment. Journal of the Formosan Medical Association 2018; 117(3): 191-196.

2.     Lin Y-C, Wu C-Y, Chang L-Y, Chen C-C, Chen H-H, Lai Y-L*, Hung S-L*. Levels of HMGB1 in gingival crevicular fluid in non-smokers and smokers with chronic periodontitis. Journal of the Formosan Medical Association2017; 116(12): 933-939.

3.     Cheng C-F, Wu K-M, Chen Y-T, Hung S-L*. Bacterial adhesion to antibiotic-loaded guided tissue regeneration membranes - a scanning electron microscopy study. Journal of the Formosan Medical Association 2015; 114(1): 35-45.

4.     Hung S-L, Lee N-G, Chang L-Y, Chen Y-T, Lai Y-L*. Stimulatory effects of glucose and lipopolysaccharide of Porphyromonas gingivalis on the secretion of inflammatory mediators from human macrophages. Journal of Periodontology 2014; 85(1):140-149.

5.     Chang L-Y, Lai Y-L, Yu T-H, Chen Y-T, Hung S-L*. Effects of areca nut extract on lipopolysaccharides-enhanced adhesion and migration of human mononuclear leukocytes. Journal of Periodontology 2014; 85(6): 859-867.

6.     Lin F-Y, Hsiao F-P, Huang C-Y, Shih C-M, Tsao N-W, Tsai C-S, Yang S-F, Chang N-C, Hung S-L* and Lin Y-W*. Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats. PLoS ONE 2014; 9(7): e102450.

7.     Lee Y-Y, Lin M-B, Cheng C-F, Chang L-Y, Liu T-Y, Hung S-L*. Inhibitory effects of the areca nut extract on expression of complement receptors and Fc receptors in human neutrophils. Journal of Periodontology 2014; 85(8): 1096-1106.

8.     Hsu M-J, Hung S-L*. Antiherpetic potential of 6-bromoindirubin-3′-acetoxime (BIO-acetoxime) in human oral epithelial cells. Archives of Virology 2013; 158: 1287-1296.

9.     Chang L-Y, Wan H-C, Lai Y-L, Chou I-C, Chen Y-T, Hung S-L*. Areca nut extracts increased the expression of cyclooxygenase-2, prostaglandin E2 and interleukin-1α in human immune cells via oxidative stress. Archives of Oral Biology 2013; 58(10): 1523-1531.

10. Lee Y-Y, Li Y-C, Hung S-L, Chen Y-C, Lee Y-H, Yang S-F*. Mineral trioxide aggregate induces the release of matrix metalloproteinase-9 by human neutrophils. Journal of Dental Sciences 2013; 8: 378-384.

11. Hung S-L, Chiang H-H, Wu C-Y, Hsu M-J, Chen Y-T*. Effects of herpes simplex virus type 1 infection on immune functions of human neutrophils. Journal of Periodontal Research 2012; 47(5): 635-644.

12. Lee Y-Y, Chan C-H, Hung S-L, Chen Y-C, Lee Y-H, and Yang S-F*. Up-regulation of nucleotide-binding oligomerization domain 1 in inflamed human dental pulp. Journal of Endodontics 2011; 37:1370-1375.

13. Wu S-Y, Chen Y-T, Chen C-W, Chi L-Y, Hsu N-Y, Hung S-L, Ling L-J*. Comparison of clinical outcomes following treatment with guided tissue regeneration using the polylactic acid barrier and the collagen membrane. International Journal of Periodontics & Restorative Dentistry 2010; 30(2): 173-179.

14. Lai Y-L*, Wu C-Y, Lee Y-Y, Chang H-W, Liu T-Y, Hung S-L. Stimulatory effects of areca nut extracts on prostaglandin E2 production by human polymorphonuclear leukocytes. Journal of Periodontology 2010; 81: 758-766.

15. Ho W-H, Lee Y-Y, Chang L-Y, Chen Y-T, Liu T-Y, Hung S-L*. Effects of areca nut extracts on the apoptosis pathways in human neutrophils. Journal of Periodontal Research 2010; 45(3):412-420.

16. Hsu M-J, Wu C-Y, Chiang H-H, Lai Y-L, Hung S-L*. PI3K/Akt signaling mediated apoptosis blockage and viral gene expression in oral epithelial cells during herpes simplex virus infection. Virus Research 2010; 153:36-43.

17. Lu H-H, Chen L-K, Cheng C-Y, Hung S-L, Lin S-C, Chang K-W*. Areca nut extract-treated gingival fibroblasts modulate the invasiveness of polymorphonuclear leukocytes via the production of MMP-2. Journal of Oral Pathology and Medicine 2009; 38(1): 79-86

18. Chang L-Y, Wan H-C, Lai Y-L, Kuo Y-F, Liu T-Y, Chen Y-T, Hung S-L*. Areca nut extracts increased expression of inflammatory cytokines, tumor necrosis factor-α, interleukin-1β, interleukin-6 and interleukin-8, in peripheral blood mononuclear cells. Journal of Periodontal Research 2009; 44: 175-183.

19. Chang L-Y, Lin J-C, Chang C-W, Ho W-H, Chen Y-T, Peng J-L, Hung S-L*. Inhibitory effects of safrole on phagocytosis, intracellular reactive oxygen species and the activity of myeloperoxidase released by human polymorphonuclear leukocytes. Journal of Periodontology 2009; 80: 1330-1337.

20. Cheng C-F, Lee Y-Y, Chi L-Y, Chen Y-T, Hung S-L and Ling L-J*. Bacterial penetration through the antibiotics-loaded guided tissue regeneration membranes. Journal of Periodontology 2009; 80: 1471-1478.

21. Chen D-C, Lee Y-Y, Yeh P-Y, Lin J-C, Chen Y-L, Hung S-L*. Eugenol inhibited the antimicrobial functions of neutrophils. Journal of Endodontics 2008; 34(2): 176-180.

22. Lai Y-L, Lin J-C, Yang S-F, Liu T-Y, Hung S-L*. Areca nut extracts reduced the intracellular reactive oxygen species and release of myeloperoxidase by human polymorphonuclear leukocytes. Journal of Periodontal Research 2007; 42(1): 69-76.

23. Chen D-C, Lai Y-L, Lee S-Y, Hung S-L and Chen H-L*. Osteoblastic response to collagen scaffolds varied in freezing temperature and glutaraldehyde crosslinking. Journal of Biomedical Materials Research Part A 2007; 80A: 399-409.

24. Lee Y-Y, Hung S-L, Pai S-F, Lee Y-H, Yang S-F*. Eugenol suppressed the expression of lipopolysaccharide-induced pro-inflammatory mediators in human macrophages. Journal of Endodontics 2007; 33(6): 698-702.

25. Hung S-L, Lin Y-J, Chien E-J, Liu W-G, Chang H-W, Liu T-Y, Chen Y-T*. Areca nut extracts-activated secretion of leukotriene B4, and phosphorylation of p38 mitogen-activated protein kinase and elevated intracellular calcium concentrations in human polymorphonuclear leukocytes. Journal of Periodontal Research 2007 42(5):393-401.

26. Lee Y-Y, Yang S-F, Ho W-H, Lee Y-H, Hung S-L*Eugenol modulates cyclooxygenase-2 expression through the activation of nuclear factor-kappa B in human osteoblasts. Journal of Endodontics 2007; 33(10): 1177-1182.

27. Hung S-L, Lee Y-Y, Liu T-Y, Peng J-L, Cheng Y-Y, Chen Y-T*. Modulation of phagocytosis, chemotaxis and adhesion of neutrophils by areca nut extracts. Journal of Periodontology 2006; 77(4): 579-585.

28. Chang L-Y, Wan H-C, Lai Y-L, Liu T-Y, Hung S-L*. Enhancing effects of areca nut extracts on production of interleukin-6 and interleukin-8 by peripheral blood mononuclear cells. Journal of Periodontology 2006; 77(12): 1969-1977.

29. Teng Y-C, Chen Y-T, Cheng Y-Y, Hung S-L*. Requirements for the upregulation of interleukin-6 by herpes simplex virus-infected gingival fibroblasts. Viral Immunology 2005; 18(1): 170-178.

30. Hung S-L, Cheng Y-Y, Peng J-L, Chang L-Y, Liu T-Y, Chen Y-T. Inhibitory effects of areca nut extracts on phagocytosis of Actinobacillus actinomycetemcomitans ATCC 33384 by neutrophils. Journal of Periodontology 2005; 76(3): 373-379.

31. Ling L-J, Ho F-C, Chen Y-T, Holborow DW, Liu T-Y, Hung S-L*. Areca nut extracts modulated expression of alkaline phosphatase and receptor activator of nuclear factor κB ligand in osteoblasts. Journal of Clinical Periodontology2005; 32(4): 353-359.

32. Hung S-L, Lin Y-W, Chen Y-T, Ling L-J. Attachment of periodontal ligament cells onto various antibiotics-loaded guided tissue regeneration membranes. International Journal of Periodontics & Restorative Dentistry 2005; 25(3): 265-275.

33. Teng Y-C, Chen Y-T, Cheng Y-Y, Hung S-L*. Requirements for the upregulation of interleukin-6 by herpes simplex virus-infected gingival fibroblasts. Viral Immunology 2005; 18(1): 170-178.

34. Ling L-J, Ho C-C, Wu C-Y, Chen Y-T, Hung S-L*. Association between human herpesviruses and the severity of periodontitis. Journal of Periodontology 2004; 75(11): 1479-1485.

35. Lai Y-L, Chen Y-T, Lee S-Y, Shieh T-M, Hung S-L*. Cytotoxic effects of dental resin liquids on primary gingival fibroblasts and periodontal ligament cells in vitro. Journal of Oral Rehabilitation 2004; 31: 1165-1172.

36. Ling L-J, Hung S-L, Lee C-F, Chen Y-T, Wu K-M. The influence of membrane exposure on the outcomes of guided tissue regeneration: clinical and microbiological aspects. Journal of Periodontal Research 2003; 38(1): 57-63.

37. Hung S-L*, Chen Y-L, Chen Y-T. Effects of safrole on the defensive functions of human neutrophils. Journal of Periodontal Research 2003; 38(2): 130-134.

38. Chen Y-T, Wang Y-H, Cheng Y-Y, Hung S-L*. Direct binding of herpes simplex virus type 1 virions to complement C3. Viral Immunology 2003; 16(3): 347-355.

39. Chen Y-T, Hung S-L, Lin L-W, Chi L-Y, Ling L-J. Attachment of periodontal ligament cells on chlorhexidine-loaded guided tissue regeneration membranes. Journal of Periodontology 2003; 74(11): 1652-1659.

40. Hung S-L*, Wang Y-H, Chen H-W, Lee P-L, Chen Y-T. Analysis of herpes simplex virus entering into cells of oral origin. Virus Research 2002; 86: 59-69.

41. Hung S-L*, Cheng Y-Y, Wang Y-H, Chang K-W, Chen Y-T. Expression and roles of herpesvirus entry mediators A and C in cells of oral origin. Oral Microbiology and Immunology 2002; 17: 215-223.

42. Hung S-L, Lin Y-W, Wang Y-H, Chen Y-T, Su C-Y, Ling L-J. Permeability of Streptococcus mutans and Actinobacillus actinomycetemcomitans through guided tissue regeneration membranes and their effects on attachment of periodontal ligament cells. Journal of Periodontology 2002; 73(8): 843-851.

43. Ling L-J, Hung S-L, Tseng S-C, Chen Y-T, Chi L-Y, Wu K-M, Lai Y-L. Association between betel quid chewing, periodontal status and periodontal pathogens. Oral Microbiology and Immunology 2001; 16: 364-369.

44. Hung S-L*, Chen Y-L, Wan H-C, Liu T-Y, Chen Y-T, Ling L-J. Effects of areca nut extracts on the functions of human neutrophils in vitro. Journal of Periodontal Research 2000; 35: 186-193.

45. Hung S-L*, Lee P-L, Chen H-W, Chen L-K, Kao C-L, King C-C. Analysis of the steps involved in Dengue virus entry into host cells. Virology 1999; 257: 156-167.

46. Gilmartin GM, Hung S-L, DeZazzo JD, Fleming ES, Imperiale MJ. Sequences regulating poly(A) site selection within the adenovirus major late transcription unit influence the interaction of constitutive processing factors with the pre-mRNA. Journal of Virology 1996; 70: 1775-1783.

47. Hung, S-L, Peng C, Kostavasili I, Friedman HM, Lambris J, Eisenberg RJ, and Cohen G. H. The interaction of glycoprotein C of herpes simplex virus types 1 and 2 with the alternative complement pathway. Virology 1994; 203: 299-312.

48. Hung, S-L, Srinivasan S, Friedman HM, Eisenberg RJ, and Cohen GH. Structural basis of C3b binding by glycoprotein C of Herpes Simplex Virus. Journal of Virology 1992; 66: 4013-4027.

49. Tung FY, Abraham S, Sethna M, Hung S-L, Sethna P, Hogue BG, Brian DA. The 9-kDa hydrophobic protein encoded at the 3' end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. Virology1992; 186: 676-83.

50. Sethna PB, Hung S-L, Brian DA. Coronavirus subgenomic replicons as a mechanism for mRNA amplification. Advances in Experimental Medicine and Biology 1990; 276: 335-340.

51. Sethna PB, Hung S-L, Brian DA. Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons. Proceedings of the National Academy of Sciences of the United States of America 1989; 86: 5626-5630.