卓文隆 教授 (Cho, Wen-Long)

美國密西根州立大學 遺傳學博士

國立陽明大學 醫學院臨床醫學研究所 / 熱帶學科 教授

經歷:

2008~Present 國立陽明大學 臨床醫學研究所及熱帶醫學科 教授 與 主任
2003~2008 國立陽明大學 熱帶醫學研究所及熱帶醫學科 所長 與 主任
2003~Present 國科會生命科學研究推動中心 審議委員
2004~Present 中國生理學雜誌 編輯委員
2005~Present 免疫與感染雜誌 編輯委員
2005~2006 中國生理學雜誌 副主編
2003~2004 國立陽明大學 研發處 副研發長
2002~ 2004 國科會寄生蟲,醫事技術,實驗診斷 學門召集人
2003/2004 外交替代役專業訓練 熱帶疾病講座
2002 - 2003 考試院公務人員高等考試暨普通考試 典試委員
2001 ~ Present 國立陽明大學 寄生蟲科及研究所 教授
1997 ~ 2000 國立陽明大學 寄生蟲學科及研究所 主任與所長
1994 ~ 2000 國立陽明大學 寄生蟲學科及研究所 副教授
* E-mail: wenlong@ym.edu.tw
* Tel: (02) 2826-7173

 

專長興趣:

  1. 院內感染包曼尼不動桿菌(Ab菌)的診斷與抗藥性研究。
  2. 熱帶醫學分子生物學。
  3. 埃及斑蚊免疫系統的分生與生化的研究。
  4. 應用遺傳工程技術來預防蟲媒熱帶疾病的傅播。
  5. 登革熱病毐的相關研究。
  6. 巴布亞紐幾內亞(PNG)醫療外交及惡性瘧原蟲抗藥基因分子研究
  7. 所羅門群島醫療外交

指導過研究生:

博士班:

  1. 已畢業:陳德禮醫師/助理教授(北榮總/陽明) ;廖建維博士(北醫/疾管局)。
  2. 未畢業:曾祥洸醫師(馬偕);周中偉醫師(關渡署立);陳昕白醫師(陽明附屬醫院);鄭敏慧醫師(北榮總);黃鈴茹醫師(北榮總) 。
  3. 未畢業:吳貞志;林詩蓓;王勇力 。

碩士班:

  1. 已畢業:傅在峰(暨南大學助理教授) ;傅彥智(北榮總);邱家憶;曹士梅;吳貞志;林詩蓓;黃憲堂;曾嘉偉;劉幸嫻;黎心穎;李怡靜;莊佳慧;王成蕙;王東弘;郝淑薏;許馨分;陳慧玲;王雅慧;陳本翰;陳俞卉;侯明華;簡妙萍;張惠雯;李秋雅(衛生署);任思穎;張薏雯;蔡尹泰;楊凱婷;侯靜茹。
  2. 未畢業:王重雅;蔡佳蓉;廖玉婷;張簡怡君;戴維萩 ;William A. Sosa Diaz。

博士後研究員: Mohideen Batcha (2003-present)

專題研究:

  1. Molecular cloning and analyses of an antibacterial peptide defensin from mosquito, Aedes aegypti. (國科會NSC84-2331-B-010-077,83/8/1-84/7/31).
  2. Expression and purification of mosquito defensin with baculovirus expression system. (國科會NSC85-2331-B-010-029, 84/8/1-85/7/31).
  3. Molecular cloning and analyses of the second defensin cDNA and the gene from mosquito, Aedes aegypti. (國科會NSC86-2314-B-010-065,85/8/1-86/7/31).
  4. Expression and regulation analyses of mosquito defensin using in situ hybridization anf transgenic Drosophila. (國科會NSC87-2314-B-010-076,86/8/1-87/7/31).
  5. Molecular cloning and studies of protein kinases involved in immune response and development from Drosophila. (國科會NSC87-2311-B-010-001-B26,86/8/1-87/7/31).
  6. Molecular cloning and studies of protein kinases from mosquito, Aedes aegypti. (國科會NSC88-2314-B-010-051,87/8/1-88/7/31).
  7. Molecular cloning and studies of protein kinases involved in immune response and development from Drosophila. (國科會NSC87-2311-B-010-001-B26,87/8/1-88/7/31).
  8. Molecular cloning and studies of AaZAP-70, AaPK38 and AaMKK3 protein kinases from mosquito, Aedes aegypti. (國科會NSC 三年計畫,88/8/1-91/7/31).
  9. Protein purification and cDNA cloning of dengue-2 virus receptor from AP61 cells. (榮陽清計畫 VTY89-P5-42 89/1-89/12).
  10. 利用蚊子基因轉殖系統來探討斑蚊抗菌蛋白DEFENSIN基因的調控 (國科會NSC 三年計畫,89/8/1-92/7/31)
  11. 埃及斑蚊分裂原活化蛋白激脢AaMEK3, AaMEK4和Aap38之磷酸化與生理功能的研究 (國科會NSC 三年計畫,91/8/1-94/7/31)
  12. 國科會寄生蟲學,醫事技術暨實驗診斷學門推動計畫 (國科會NSC91/1/1-92/12/31)
  13. 登革二型病毒受體的特性分析與純化 (國科會NSC 三年計畫,92/8/1-95/7/31)
  14. 衛生署疾管局新興寄生蟲感染症之分子檢驗技術開發
  15. 巴布亞紐幾內亞(PNG)醫療外交計畫
  16. 所羅門群島醫療外交計畫

國際期刊論著:

  1. Raikhel, A. S., Dhadialla, T. S., Cho, W. -L., Hays, A. R. and Koller, C. N. (1990) Biosynthesis and endocytosis of yolk proteins in the mosquito. In : Molecular Insect Science (Eds. Hagedorn, H. H., Hildebrand, J. G., Kidwell, M. G., and Law, J. H.), Plenum, New York, 147-154.
  2. Cho, W.-L., Dhadialla, T. S. and Raikhel, A. S. (1991) Purification and characterization of a lysosomal aspartic protease with cathepsin D activity from the mosquito. Insect Biochem., 21, 165-176.
  3. Cho, W. -L., Deitsch, K. W. and Raikhel, A. S., (1991) An extraovarian protein accumulated in mosquito oocytes is a carboxypeptidase activated in embryos. Proc. Natl. Acad. Sci. (U. S. A.), 88, 10821-10824.
  4. Cho, W. -L., Raikhel, A. S. (1992) Cloning of cDNA for mosquito lysosomal aspartic protease. J. Biol. Chem. 267, 21823-21829.
  5. Cho, W. -L., Deitsch, K. W., Chen, J. S., Ditmer, N. T. and Raikhel, A. S. (1993) Molecular Studies of Mosquito Vitellogenesis, in: Host Regulated Developmental Mechanisms In Vector Arthropods (Eds. Borovsky, D. and Spielman, A.) 48-55.
  6. Chen, J. -S., Cho, W. -L., Raikhel, A. S. (1994) Analysis of mosquito vitellogenin cDNA. J. Mol. Biol. 237, 641-647.
  7. Deitsch, K. W., Dittmer, N., Kapitskaya, Chen, J. -S., Cho, W. -L., and Raikhel, A. S. (1995) Regulation of gene expression by 20-hydroxyecdysone in the fat body of Aedes aegypti (Diptera: Culicidae) Eur. J. Entomol. 92, 237-244.
  8. Cho, W. -L., Kapitskaya, M. Z. and Raikhel, A. S. (1995) Mosquito ecdysteroid receptor: Analysis of the cDNA and expression during vitellogenesis. Insect Biochem. Molec. Biol., 25, 19-27
  9. Sappington, T. W., Kokoza, V. A., Cho, W. -L., and Raikhel, A. S. (1996) Molecular characterization of the mosquito vitellogenin receptor reveals unexpected high homology to the Drosophila yolk protein receptor. Proc. Natl. Acad. Sci. (USA) 93, 8934-8939.
  10. Cho, W. -L, Fu, Y. -C, Chen, C. -C., and Ho, C. -M. (1996) Cloning and characterization of cDNAs encoding the antibacterial peptide, defensin A, from mosquito , Aedes aegypti. Insect Biochem. Molec. Biol. 26, 395-402.
  11. Cho, W. -L., Fu, T. -F., Chiou, J. -Y., and Chen, C. -C. (1997) Molecular characterization of a defensin gene from mosquito, Aedes aegypti. Insect Biochem. Molec. Biol. 27, 351-358.
  12. Cho, W. -L., Liu, S. H., Lee, C. H., Kuo, C. C., Chang, C. I., and Chen, C. C., (1998) Molecular cloning, characterization and tissue expression of phenoloxidase cDNA from mosquito Armigeres subalbatus inoculated with Dirofilaria immitis Microfilariae, Insect Molecular Biology, 7, 1-10.
  13. Cho, W. -L., Fu, T. -F., Chiou, J. -Y., and Chen, C. -C. (1997) Molecular characterization of a defensin gene from mosquito, Aedes aegypti. Insect Biochem. Molec. Biol. 27, 351-358.
  14. Cho, W. -L., Liu, S. H., Lee, C. H., Kuo, C. C., Chang, C. I., and Chen, C. C., (1998) Molecular cloning, characterization and tissue expression of phenoloxidase cDNA from mosquito Armigeres subalbatus inoculated with Dirofilaria immitis Microfilariae, Insect Molecular Biology, 7, 1-10.
  15. Chiou, J. -Y., Huang, S. -J., Huang, S. -T., Cho, W. -L. (1998) Identification of immune-related protein kinases from mosquitoes (Aedes aegypti), J. Biomedical Science, 5, 120-126
  16. Huang, A. -M., Chang, T. -J., Cho, W. L., Chou, C. -K. (1998) From mosquito to man: Identification of a novel protein kinase, HsHPK, which is highly expressed in human hepatoma tissues. J. Biomedical Science, 5, 135-140
  17. Kapittskaya, M. Z., Dittmer, N. T., Deitsch, K. W., Cho, W. -L., Taylor, D. G., Leff, T., Raikhel, A. S., (1998) Three isoforms of an HNF-4 transcription factor with tissue- and stage-specific expression in the adult mosquito, J. Biol. Chem. 273, 29801-29810.
  18. Cho,W. -L., Tsao, S. -M., Hays, A. R., Walter, R., Chen, J. -S., Snigirevskaya, E. S., and Raikhel, A. S. (1999) Mosquito cathepsin B-like protease involved in embryonic degradation of vitellin as a latent extraovarian precursor, J. Biol. Chem. 274, 13311-13321.
  19. Kau, M. -M., Chen, J. -J., Wang, S. -W., Cho, W. -L., Wang, P. S. (1999) Age-related impairment of aldosterone secretion in zona glomerulosa cells of ovariectomized rats. J. Investigative Medicine, 47, 425-432.
  20. Lo, Ming-Jae, Kau, Mei-Mei, Cho, W. -L., Wang, P. S. (2000) Aging effects on the secretion of corticosterone in male rats. J. Investigative Medicine. 48, 335-342.
  21. Cho,W. -L., Tsao, S. -M., Hays, A. R., Walter, R., Chen, J. -S., Snigirevskaya, E. S., and Raikhel, A. S. (1999) Mosquito cathepsin B-like protease involved in embryonic degradation of vitellin as a latent extraovarian precursor, J. Biol. Chem. 274, 13311-13321.
  22. *Vladimir Kokoza, *Abduelaziz Ahmed, *Cho, W. -L., Nijole Jasinskiene , Anthony A. James and Alexander Raikhel (2000) Engineering blood meal-activated systemic immunity in the mosquito vector. Proc. Natl. Acad. Sci. (USA) 97, 9144-9149. (NSC 90-2320-B-010-093) (*Cho, W.L., Kokoza, K. and Ahmed, A. have equal contribution indicated in the paper)
  23. Cho, W. -L. and Raikhel, A.S. (2001) A novel function of 20-hydroxyecdysone: translational repression of the lysosomal protease mRNA in the mosquito fat body. Insect Biochem. Molec. Biol. 31, 283-288.
  24. Chiao, Y. C., Cho, W. L. and Wang, P.S. (2002) Inhibition of testosterone production by propylthiouracil in rat leydig cells. Biology of Reproduction, 67, 416-422.
  25. Huang, S. J., Li, S. Y., Wu, R. C. C., Shaio, M. F., Wang, P. S. and Cho, W. L. (2003) An immune signaling kinase AaMEK3 from mosquitoes: cDNA cloning and characterization. Insect Molec. Biol.12, 595-603.
  26. *Bartholomay, L. C., *Cho, W. L., Rocheleau, T. A. Boyle, J. P., Beck, E. T., Fuchs, J. F., Liss, P., Rusch, M., Butler, K. M., Wu, R. C. C., Lin, S. P., Kou, H. Y., Tsao, I. Y., Huang, C. Y., Wu, T. T, . Hsiao, K. J., Tsai, S. F., Yang, U. C., Nappi, A. J., Perna, N. T., Chen, C. C., and Christensen, B. M. (2004) Toward a description and transcriptomes of immune-activated hemocytes from the mosquito vectors, Aedes aegypti and Armigeres subalbatus. Infection and Immunity, 72: 4114-4126. * indicates the equal contributions.
  27. Liu, I.H, Chung, Y.M, Chen, S.J, Cho, W.L. (2006) Necrotizing Retinitis Induced by Angiostrongylus cantonensis. American journal of ophthalmology, 141: 577-579
  28. Chen, T. L., Chuang, C. L., Siu, L. K., Fung, C. P., and Cho, W. L. (2007) Genomic species identification is important to delineate the pathological characteristics of Acinetobacter in tunneled, cuffed hemodialysis catheter-related bacteremia. Nephrology Dialysis Transplantation, 22, 936-938.
  29. Wu, R.C.C., Shaio, M.F., and Cho, W. L. (2007) A p38 MAP kinase regulates the expression of the Aedes aegypti defensin gene in mosquito cells. Insect Molecular Biology, (16, 389-399)
  30. Chen, T. L., Siu, L. K., Wu, R. C. C., Shaio, M. F., Huang, L. Y., Fung, C. P., Lee, C. M. and Cho, W. L. (2007) Comparison of one-tube multiplex PCR and automated ribotyping for rapid identification of Acinetobacter baumannii. Clinical Microbiology and Infection, 13, 801-806
  31. George F. Mayhew, Lyric C. Bartholomay, Hang-Yen Kou, Thomas A. Rocheleau, Jeremy F. Fuchs, Matthew T. Aliota, I-Yu Tsao, Chiung-Yen Huang, Tze-Tze Liu, Kwang-Jen Hsiao, Shih-Feng Tsai,Ueng-Cheng Yang, Nicole T. Perna, Wen-Long Cho, Bruce M. Christensen and Cheng-Chen Chen. (2007) Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus. BMC Genomics, 8, 462-
  32. Han-Yueh Kuo, Dung-Hung Chiang, Chien-Chun Wang, Te-Li Chen, Chang-Phone Fung, Chih-Pei Lin, Wen-Long Cho, Cheng-Yi Liu (2008) Clinical significance of Blastocystis hominis: an experience from a medical center in northern Taiwan. Journal of Microbiology, Immunology and Infection, 41, 222-226.
  33. Te-Li Chen, Roy Chen-Chih Wu, Men-Fang Shaio, Chang-Phone Fung, and Wen-Long Cho (2008) Acquisition of a Plasmid Borne blaOXA-58 Gene with an Upstream IS1008 Insertion Conferring a High Level of Carbapenem Resistance to Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy, 52, 2573-2580.
  34. Li-Yueh Huang; Te-Li Chen; Po-Liang Lu; Che-An Tsai; Wen-Long Cho; Fang-Yee Chang; Chang-Phone Fung; Leung Kei Siu (2008) Dissemination of Multidrug-Resistant Acinetobacter baumannii Carrying Class 1 Integrons in Taiwan. Clinical Microbiology and Infection.14, 1010-1019.
  35. Chen HP, Lin JC, Yang SP, Lan YC, Weng WS, Tsai CH, Ho DM, Liu CY, Cho WL, Chan YJ. (2008) The type-2 variant of human cytomegalovirus glycoprotein N (gN-2) is not the rarest in the Chinese population of Taiwan: Influence of primer design. J Virol Methods, 151, 161-164.
  36. Chien-Wei Liao, Wen-Long Cho,* Ting-Chang Kao, Kua-Eyre Su, Yun-Ho Lin, Chia-Kwung Fan* (2008) Blood-brain barrier impairment with enhanced SP, NK-1R, GFAP, and Claudin-5 expressions in experimental cerebral toxocariasis. Parasite Immunology, 30, 525-534. * indicates corresponding author.
  37. Chien-Wei Liao, Chia-Kwung Fan, Ting-Chang Kao, Dar-Der Ji, Kua-Eyre Su, Yun-Ho Lin, Wen-Long Cho (2008) Brain injury-associated biomarkers of TGF-β1, S100B, GFAP, NF-L, tTG, AβPP, and tau were concomitantly enhanced and the UPS was impaired during acute brain injury caused by Toxocara canis in mice. BMC Infectious Diseases, 24, 84-.99.
  38. Te-Li Chen, Leung-Kei Siu, Yi-Tzu Lee, Chien-Pei Chen, Li-Yueh Huang, Roy Chen-Chih Wu, Wen-Long Cho, and Chang-Phone Fung. (2008) Acinetobacter baylyi as a Pathogen for Opportunistic Infection. Journal of Clinical Microbilogy, 46, 2938-2944.,
  39. Dung-Hung Chiang, Chien-Chun Wang, Han-Yueh Kuo, Hsin-Pai Chen, Te-Li Chen, Fu-Der Wang, Wen-Long Cho, Cheng-Yi Liu (2009) Risk factors of attributable mortality for blood-stream infection of Acinetobacter baumannii with precise species identification. Journal of Microbiology, Immunology and Infection (in press).