Dr. Shih-Tung Liu, Professor

Department of Microbiology and Immunology, Chang-Gung University
Phone: 886-3-211-8800 #5133
E-mail: cgliu@mail.cgu.edu.tw


1971, B.S.@ Botany
@@@@@@ National Chung-Hsing University, Taiwan

1977, Ph.D.@Molecular Biology
@@@@@@ University of California, Berkeley


1. Regulation of BRLF1 Transcription of Epstein-Barr Virus by MCAF

2. Genes Involved in the Late Stage of Lytic Development of Epstein-Barr

3. Virus Capsid assembly and DNA encapsidation by Epstein-Barr virus

4. Proteins interacting with Rta of Epstein-Barr virus Publications


1. S.-T. Liu, D. Grunner, and C. A. Knight. 1978. Bound form indole-3-acetic acid synthesis in tumorous and non-tumorous species of Nicotiana. Plant Physiol. 61: 50-55.

2. S.-T. Liu, C. D. Katz, and C. A. Knight. 1978. Indole-3-acetic acid synthesis in tumorous and non-tumorous species of Nocotiana. Plant Physiol. 61: 743-747.

3. S.-T. Liu, and C. I. Kado. 1979. Indole-3-acetic acid production. A plasmid function of Agrobacterium tumefaciens C58. Biochem. Biophy. Res. Commun. 90: 171-178.

4. C. I. Kado and S.-T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bioteriol. 145: 1365-1371.

5. S.-T. Liu, K. L. Perry, C. Shardl, and C. I. Kado. 1982. Agrobacterium Ti plasmid indole acetic acid gene is required for crown gall oncogenesis. Proc. Natl. Acad. Sci. USA. 79: 2812-2816.

6. K. L. Perry, T. A. Simonitch, K. L. Harrison-Lavoie, and S.-T. Liu. 1986. Cloning and regulation of yellow-pigmant genes of Erwinia herbicola. J. Bacteriol. 168: 607-612.

7. A. H. Goldstein, and S.-T. Liu. 1987. Molecular Cloning and regulation of a mineral phosphate solubilizing (MPS) gene from Erwinia herbicola. Bio-Technol. 5: 72-74.

8. S.-Y. Wang, S.-T. Liu, S.-J. Wang, and C.-K. Ho. 1989. Induction of differentiation in HL-60 cells by retinoic acid and lymphocyte-derived differentiation-inducing factor but not by recombinant G-CSF and GM-CSF. Leukemia Res. 13: 1091-1097.

9. Y.-S. Chang, L.-Y. Shih, C.-L. Peng, S.-S. Chen and S.-T. Liu. 1990. Characterization of two newly established EBV-containing lymphoblastoid cell lines from patients with myeloid leukemias. Leukemia Res. 14: 309-320.

10. L. H. Hwang, B. F. Chen, H. M. Lin, L. L. Soong, Y. S. Jou, W. C. Chang, and S.-T. Liu. 1990. The striking effects of spacers and terminators sequences on the synthesis of porcine growth hormone in Escherichia coli. Biotech. Lett. 12: 335-340.

11. Y.-S. Chang, Y.-S. Tyan, S.-T. Liu, M.-S. Tsai and C.-C. Pao. 1990. Detection of Epstein-Barr virus DNA sequences in nasopharyngeal carcinoma cells by Enzymatic DNA amplification. J. Clin. Microbiol. 28: 2398-2402.

12. L. H. Hwang, H. Tsai, and S.-T. Liu. 1990. High level expression of porcine growth hormone in Escherichia coli from an expression vector containing bacterial phage lambda Pl promoter and the N gene untranslated region. Biochem. Biophy. Res. Commun. 173: 711-717.

13. L. Y. Lee, and S.-T. Liu. 1991. Characterization of the yellow pigment genes of Erwinia herbicola. Mol. Microbiol. 5: 217-224.

14. S.-T. Liu, R.-N. Lee, D.-C. Wang, S.-F. Chang, S.-C. Chiang, W.-C. Ho, Y.-S. Chang, and S.-S. Lai. 1991. Rapid detecetion of hog cholera virus in tissues by the polymerase chain reaction. J. Virol. Methods. 35: 227-236.

15. Y.-S. Chang, M.-L. Chen, R.-C. Wu, P.-J. Chang, C.-H. Shu and S.-T. Liu. 1991. Sequence analysis of the BZLF 1 gene of Epstein-Barr virus derived from a new variant isolated in Taiwan. Nucleic Acids Res. 20: 139.

16. C. H. Shu, Y. S. Chang, C. L. Liang, S.-T. Liu, C. Z. Lin, and P. Chang. 1992. Detection of type A and type B EBV in normal individuals and patients with head and neck carcinomas in Taiwan. J. Virol. Methods. 38: 123-130.

17. M.-L. Chen, C.-N. Tsai, C.-R. Huang, C.-L. Liang, C.-H. Shu, Dov Sulitzanu, S-T Liu and Y.-S. Chang. 1992. Cloning and characterization of the Latent Membrane Protein ( LMP) of a specific Epstein-Barr virus variant derived from the nasopharyngeal carcinoma in the Taiwanese population. Oncogene 7: 2131-2140.

18. K.-Y. To, C.-Y. Li, Y.-S. Chang and S.-T. Liu. 1992. Direct sequencing of tobacco chloroplast genome by the polymerase chain reaction. Plant Mol. Biol. 19: 1073-1077.

19. S.-T. Liu, L.-Y. Lee, C.-Y. Tai, C.-H. Hung, Y.-S. Chang, James H. Wolfram, Robert Rogers and Alan H. Goldstein. 1992. Cloning of an Erwinia herbicola gene necessary for gluconic acid production and enchanced mineral phosphate solubilization in Escherichia coli HB101: Nucleotide sequence and probable involement in biosynthesis of the coenzyme pyrroloquinoline quinone. J. Bacteriol. 174: 5814-5819.

20. Y.-S. Chang, Y.-J. Lin, C.-N. Tsai, C.-H. Shu, M.-S. Tsai, K.-B. Choo and S.-T. Liu. 1992. Detection of mutations in the p53 gene in human head and neck carcinomas by single strand conformation polymorphism analysis. Cancer Lett. 67: 167-174.

21. K.-Y. To, C.-I Liu, S.-T. Liu, and Y.-S. Chang. 1993. Detection of point mutations in chloroplast genome by single-stranded conformation polymorphism analysis. Plant J. 3: 183 -186.

22. Y.-S. Tyan, S.-T. Liu, W.-R. Ong, M.-L. Chen, C.-H. Shu and Y.-S. Chang. 1993. Detection of Epstein-Barr Virus and Human papillomavirus in head and neck tumors in Taiwan. J. Clin. Microbiol. 31: 53- 56.

23. S.-T. Liu. 1993. Carotenoid-biosynthesis genes as a genetic marker for the purpose of gene cloning. Biochemi. and Biophy. Res. Commun. 195: 259-263.

24. K.-Y. To, E.-M. Lai, L.-Y. Lee, T.-P. Lin, C.-H. Hung, C.-L.Chen and S.-T. Liu. 1994. Analysis of the gene cluster encoding carotenoid biosynthesis in Erwinia herbicola Eho13. Microbiology 140: 331-339.

25. T.-P. Lin, E.-M. Lai, K.-Y. To, Y.-S. Chang, S.-T. Liu. 1994. Transcriptional activation of flanking sequences by Tn1000 insertion. Mol. Gen. Genet. 245: 417-423.

26. L.-K. Chang, C.-L.Chen, Y.-S. Chang, J. S.-M. Tschen, Y.-M. Chen and S.-T. Liu. 1994. Construction of Tn 917acl, a transposon useful for mutagenesis and cloning of Bacillus subtilis genes. Gene 150: 129-134.

27. K.-C. G. Jeng, C.-Y. Hsu, M.-T. Liu, T.-T. Chung and S.-T. Liu. 1994. Prevalence of Taiwan variant of Epstein-Barr virus in throat washings from patients with head and neck tumors in Taiwan. J. Clin. Microbiol. 32: 28-31.

28. M.-L. Chen, R.-C. Wu, S.-T. Liu, and Y.-S. Chang. 1995. Characterization of 5'-upstream sequence of the latent membrane protein 1 (LMP1) gene of Epstein-Barr virus identified in nasopharyngeal carcinoma tissues. Virus Res. 37: 75-84.

29. M.-L. Chen, N.-C.Hsu, S.-T. Liu, and Y.-S. Chang. 1995. Identification of an internal promoter of the latent membrane protein 1 gene of Epstein-Barr virus. DNA Cell Biol. 14: 205-211.

30. Y.-S. Chang, I.-J. Su, P.-J. Chung, C.-H. Shu, C.-K. Ng, S.-J. Wu, and S.-T. Liu. 1995. Detection of an Epstein-Barr virus variant in T-cell lymphoma tissues identical to the distinct strain observed in nasopharyngeal carcinoma in the Taiwanese. Int. J. Cancer. 62 673-677.

31. L.-W. Chen, L.-S. Lin, Y.-S. Chang and S.-T. Liu. 1995. Functional analysis of EA-D of Epstein-Barr Virus. Virology 211:593-597.

32. C.-L. Chen, L.-K. Chang, Y.-S. Chang, S.-T. Liu, and J. S.-M. Tschen. 1995. Transposon mutagenesis and cloning of the genes encoding the enzymes of fengycin biosynthesis in Bacillus subtilis. Mol. Gen. Genet. 248:121-125.

33. J.-F. Fu, H.-C. Chang, Y.-M. Chen, and S.-T. Liu. 1995. Sequence analysis of an Erwinia stewartii plasmid pSW100. Plasmid 34:75-84.

34. J.-F. Fu, H.-C. Chang, Y.-M. Chen, Y.-S Chang and S.-T. Liu. 1996. Characterization of the replicon of plasmid pSW500 of Erwinia stewartii. Mol. Gen. Genet: 250:699-704.35. S.-N. Li, Y.-S. Chang and S.-T. Liu. 1996. Effect of a 10-amino acid deletion on the oncogenic activity of latent membrane protein 1 of Epstein-Barr virus. Oncogene 12:2129-2135.

36. J.-S. Shiu, M.-H. Chang, S.-T. Liu, W.-C. Ho, S.-S. Lai, T.-J. Chang, Y.-S. Chang. 1996. Molecular cloning and nucleotide sequence determination of three envelope genes of classical swine fever virus Taiwan isolate p97. Virus Res. 41:173-178.

37. J.-S. Shiu, S.-T. Liu, T.-J.Chang, W.-C. Ho, S.-S. Lai, and Y.-S. Chang. 1997. The presence of RNA splicing signals in the cDNA construct of the E2 gene of classical swine fever virus (CSFV) affected its expression. J. Virol. Methods. 69:223-230.1997.

38. T.-S. Yeh, S.-N. Li, C.-J. Wu, S.-T. Liu, C.-L. Meng and Y.-S. Chang. 1997. Sequence variations between two Epstein-Barr virus LMP1 variants have no effect on the activation of NF-B activity. DNA Cell Biol. 16:1311-1319. 1997.

39. J.-F. Fu, S.-W. Ying, and S.-T. Liu. 1997. Cloning and Characterization of the ori region of pSW1200 of Erwinia stewartii. Plasmid 38:141-147. 1997.

40. G.-H. Lin, C. -L. Chen, J. S. -M, Tschen, S. -S. T, Y. -S. Chang, and S.-T. Liu. 1998. Molecular cloning and Characterization of fengycin synthetase gene fenB from Bacillus subtilis. J. Bacteriol. 180: 1338-1341.

41. P.-J. Chang, Y.-S. Chang, and S.-T. Liu. 1998. Role of Rta in the translation of bicistronic BZLF1 of Epstein-Barr virus. J. Virol. 72: 5128-5136.

42. J.-F. Fu, J.-M. Hu, Y.-S. Chang and S.-T. Liu. 1998. Isolation and characterization of plasmid pSW200 from Erwinia stewartii. Plasmid 40:100-112.

43. C.-J. Wu, C.-Y. Leu, S.-T. Liu, K.-P. Chow, C.-L. Meng, and Y.-S. Chang. 1998. Transcriptional activation of NF-B activity by Epstein-Barr virus (EBV) LMP1 as a selective therapeutic strategy for EBV-associated disease. Gene Ther. 5:905-912.

44. P.-J. Chang, Y.-S. Chang and S.-T. Liu. 1998. Characterization of the BcLF1 promoter in Epstein-Barr virus. J. Gen. Virol. 79:2003-2006.

45. T.-P. Lin, C.-L. Chen, L.-K. Chang, J. S.-M. Tschen, and S.-T. Liu. 1999. Functional and Transcriptional Analyses of a Fengycin Synthetase Gene, fenC, from Bacillus subtilis. J. Bacteriol. 181:5060-5067.

46. C.-H. Hung, M.-J. Kwan, and S.-T. Liu. 1999. A Cosmid System for the Analysis of Lytic Replication of Epstein-Barr Virus. Anal. Biochem. 272:277-280.

47. C.-H. Hung, and S.-T. Liu. 1999. Characterization of the Epstein-Barr virus BALF2 promoter. J. Gen. Virol. 80:2747-2750.

48. S.-Y Hsieh, X.-J. Meng, Y.-H. Wu, S.-T. Liu, Albert W. Tam, D.-Y. Lin, and Y.-F. Liaw. 1999. Identity of a Novel Swine Hepatitis E Virus in Taiwan Forming a Monophyletic Group with Taiwan Isolates of Human Hepatitis E Virus. J. Clin. Microbiol. 37:3828-3834.

49. L.-K. Chang, and S.-T. Liu. 2000. Activation of the BRLF1 Promoter and Lytic Cycle of Epstein-Barr Virus by Histone Acetylation. Nucleic Acids Res. 28:3918-3925.

50. P.-J. Chang, and S.-T. Liu. 2001. Function of the Intercistronic Region of the BRLF1-BZLF1 Bicistronic mRNA in Translating the Zta Protein of the Epstein-Barr Virus. J. Virol. 75:1142-1151.

51. C.-Y. Wu, J.-F. Fu, and S.-T. Liu. 2001. The replicon of pSW800 from Pantoea stewartii. Microbiology 147:2757-2767.

52. H.-Y. Shu, G.-H Lin, Y.-C. Wu, J. S.-M. Tschen, and S.-T. Liu. 2002. Amino Acids Activated by Fengycin Synthetase FenE. Biochem. Bioph. Res. Co. 292:789-793.

53. L.-K. Chang, T.-T. Wei, Y.-F. Chiu, C.-P. Tung, J.-Y. Chung, S.-K. Hung, C. Li, and S.-T. Liu. 2003. Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate. Biochem. Bioph. Res. Co. 301:1062-1068.

54. L.-K. Chang, Y.-H. Lee, T.-S. Cheng, Y.-R. Hong, P.-J. Lu, J.-J. Wang, W.-H. Wang, C.-W. Kuo, Steven S.-L. Li, and S.-T. Liu. 2004. Posttranslational modification of Rta of Epstein-Barr virus by SUMO-1. J. Biol. Chem. 279:38803-38812.

55. T.-P. Lin, C.-L. Chen, H.-C. Fu, C.-Y. Wu, G.-H. Lin, S.-H. Huang, L.-K. Chang, and S.-T. Liu. 2005. Functional analysis of fengycin synthetase FenD. BBA-Gene. Struct. Expr. 1730:159-164.

56. L.-K. Chang, J.-Y. Chung, Y.-R. Hong, Takaya Ichimura, Mitsuyoshi Nakao, and S.-T. Liu. 2005. Activation of Sp1-meiated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1. Nucleic Acids Res. 33:6528-6539.

57. C. Li, R.-S. Chen, S.-K. Hung, Y.-T. Lee, C.-Y. Yen, Y.-W. Lai, R.-H. Teng, Biehuoy Shieh, C.-P. Tung, T.-T. Wei, and S.-T. Liu. 2005. Detecting Epstein-Barr infection and gene expression in human tumors by microarray analysis. J. Virol. Methods. (in press)

Members of Laboratory