Dr. Shie-Liang Hsieh, Director / Professor

Phone: 886-2-2826-7161
E-mail: slhsieh@ym.edu.tw


1985, M.D.@ School of Medicine
@@@@@@ National Yang-Ming University, Taiwan

1992, Ph.D.@University of Oxford, UK

1993 - 1994@Robert Wool Johnson Fellow
@@@@@@ Stanford University School of Medicine, USA


1. Immunomodulation of Dendritic cells and macrophages by deco receptor         3 (DcR3).
    a) Role of DcR3 in the differentiation of type I vs type II macrophages (M1
        vs. M2), as well as tumor associated macrophages (TAMs).
    b) Functions of DcR3 to promote stem cell release for tissue reparing.

2. Host-Pathogen Interaction
    a) Identification of innate immunity receptors interacting with viruses and
        other pathogens.
    b) Functional study of innate immunity receptors (C-type lectin, SIGLEC,
        TREM and others ) in acute (dengue virus, influenza virus) and chronic
        (hepatitis B and C viruses) viral infections.
    c) Fingerprinting of polysaccharides isolated from Chinese herb drug.

3. Cancer Stem Cells.
    a) Modulation of glycan structure on cancer stem cells.
    b) Interaction between cancer stem cells with innate immunity receptors.



(*: corresponding author)

1. Yeh, N.H., Chen, C.M. and Hsieh, S.L. (1988) Rapid Identification of Monoclonal Antibodies to Differential Antigens by Dot Immunobinding Assay. Chinese J. Microbiol. and Immunol. 21, 210-223

2. Hsieh, S.L., and Campbell, R.D. (1991) Evidence that G7a gene in the human MHC Class III region is valyl-tRNA synthetase. The Biochemical Journal 278, 809-81

3. Zhu, Z.B., Hsieh, S.L., Bentley, D.R., Campbell, R.D. and Volanakis, J.E. (1992) A variable number of tandem repeats (VNTR) locus within the human complement C2 gene is associated with a retroposon derived from a human endogenous retrovirus. J. Exp. Med. 175, 1783-1787

4. Sargent C.A., Anderson M.J., Hsieh, SL, Kendall E, Escobar N.G., Campbell R.D. (1994) Characterization of the novel gene G11 lying adjacent to the complement C4A gene in the human major histocompatibility complex. Human Molecular Genetics 3, 481-488

5. Hsieh, S.L., March, R.E., Khanna, A., Cross, S.J., Campbell, R.D. (1997) Mapping of 10 novel microsatellites in the MHC class III region: Application to the study of diseases. J. of Rheumatology 24, 220-222

6. Wu M.Y., Hsu TL, Lin W.W., Campbell RD, Hsieh, SL * (1997) Serine/threonine kinase activity associated with the cytoplasmic domain of the lymphotoxin-beta receptor in HepG2 cells. J. Biol. Chem. 272, 17154-17159

7. Zhai Y, Guo, R, Hsu, TL, Ni, J, Kwon, BS, Yu, GL, Jiang, GW, Tan, J, Lu, J, Rojas, L., Endress, G, Xing, L, Zhu, F, Wang, S., Oh, KO, Reiner R, Lippman, ME, Garotta, G, Hsieh, SL ** and Yang, D (1998) LIGHT, A Novel Ligand for Lymphotoxin-beta Receptor and HVEM Induces Apoptosis and Displays Anti-tumor Activity In Vivo. J. Clin. Investigation, 102, p1142-1151, 1998 **shared senior author

8. Gomez-Escobar, N., Chou, C.F., Lin, W.W., Hsieh, S.L, Duncan, R.D. (1998) The G11 gene located in the Major Histocompatibility Complex encodes a novel nuclear Serine/Threonine protein kinase J. Biol. Chem. 273, 30954-30960

9. Wu, M.Y., Wang, P.Y., Han, S.H., Hsieh, SL*. (1999) Cytoplasmic domain of the Lymphotoxin-beta Recetpor Mediates Cell Death in HeLa cells J. Biol. Chem. 274, 11868-11873

10. Tamada K, Shimozaki K, Chapoval AI, Zhai Y, Su J., Chen, SF, Hsieh, SL, Nagata S, Ni, J, Chen, LP (2000) LIGHT, a TNF-Like Molecule, Costimulates T Cell Proliferation and Is Required for Dendritic Cell-Mediated Allogeneic T Cell Response. J. Immunology, 164, p 4104-4110

11. Hsieh, S.L.**., Chen, N. J., Hermann, A., Lee, K.S., Wu, S.C., Silver., Arai, N., Fox, H., McDevitt .H. Transgenic Mice Expressing TH1 and TH2 Surface Markers (2000) Molecular Immunology, 37, p 181-193 **first author and shared correspondence author

12. Chen, W.H., Lin, Y.L., Liao, C.L. and Hsieh, SL.* Modulatory Effects of the Human Heat Shock Protein 70 on DNA Vaccination. (2000) J. Bio. Sci, 7. p 412-419

13. Chen MC, Hsu TL, Lu, TY, Chou, CK, Hsieh SL* (2000) Overexpression of Bcl-2 enhances LIGHT and IFN-gama-mediated Apoptosis in Hep3BT2 Cells J. Biol.Chem, 275, p38794-38801

14. Tu, C.F, Hsieh, S.L., Lee, J.M., Yang, L.L., Sato, T, Lee, K.H., Weng, C.N., Mao, S. J., Tsuji, K., Lee, C. J. (2000) Successful generation of transgenic pigs for human decay-accelerating factor and human leucocyte antigen DQ. Transplant. Proc. 32, 913-915

15. Huang, T.H., Wu, P.I., Lee, C.N., Huang, H., Hsieh, S.L, Kung J, Tao, MH (2000) Enhanced antitumor immunity by fusion of CTLA-4 to a self tumor antigen. Blood, 96, 3663-3670

16. Chen N.J., Huang, M.W., Hsieh, S.L.* Enhanced Secretion of Interferon-gamma by Activated Th1 Cells Through Reverse Signaling via TRANCE (2001) J. Immunology 166: 270-276.

17. Chen, B.C., Hsieh, S.L., Lin W.W. (2001) Involvement of protein kinases in the potentiation of lipopolysaccharide-induced inflammatory mediator formation by thapsigargin in peritoneal macrophages (2001) J. Leuko. Biol. 69, 280-288

18. Hsu, P.N., Lin, H.H., Chen, N.J., Wu, K.M., Tu, C.F., Tsai, H.F. and Hsieh, S.L. * (2001) Human FasL gene does not induce inflammation in pancreas but is unable to protect islet graft from transplantation rejection in pancreas-specific hFasL transgeneic mice J. Bio. Sci, 8, 262-269

19. Chou, A., Lin., L.L., Tsai, H.F., Hsieh, S.L., Hsu, P.I. and Hsu, P.N. (2001) Enhanced proliferation and increased interferon-g production in T cells by signal transduced through TRAIL. (2001) J. Immunology 167, 1347-1352

20. Chow KP, Lu HC, Chou HF, Liu HP, Hsieh SL, Chang YS, Choo KB. (2002) Induction of chemosensitivity in nasopharyngeal carcinoma cells using a human papillomavirus regulatory sequence and the thymidine kinase gene. J Biomed Sci.;9:41-6.

21. Huang, YL., Liu, CJ., Chiu, AW., Wang, YC., Huan, SK., Lee, FL., Chen, SJ., Hsu, WM., Hsieh, S.L. (2002) A Feasible Tool to Detect mRNA Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Human Tenons Capsule. Ophthalamic Reserach.34:375

22. Hung, S. C., Chen, N. J., Hsieh, S.L., Li, H., Ma, H. Li., Lo., WH (2002).Isolation and characterization of size-sieved stem cells from human bone marrow . Stem Cells. 20, 249-258

23. Hsu, T.L., Chang, Y.C., Chen, S.R., Chen, S.R., Liu Y.C., Chiu, A.W., Chio, C.C., Chen, L. Hsieh, S.L.* (2002) Modulation of Dendritic Cell Differentiation and Maturation by Decoy Receptor 3 (DcR3). J. Immunology 168, 4846-4853

24. Chang, Y.H., Hsieh, S.L., Chen, M.C., Lin, W.W. (2002) Lymphotoxin-beta Receptor Induces Interleukin 8 Gene Expression via NF-kB and AP-1 Activation. Exp. Cell Res. 278, 166-174, 2002

25. Chen MC, Hwang MJ, Chou YC, Chen WH, Cheng G, Nakano H, Luh TY, Mai SC, Hsieh, S.L.* (2003) The Role of ASK1 in Lymphotoxin-beta Receptor-Mediated Cell Death. J. Biol. Chem 278, 16073.

26. Hsu, MJ, Lin, W.W., Tsao, W.C., Hsu, T.L., Chang, Y.C., Chiu, A.W., Chio, C.C., and Hsieh, S.L.* (2004) Enhanced Adhesion of Monocytes via Reverse Signaling Triggered by Decoy Receptor 3 (DcR3). Exp. Cell Res. 15;292(2):241-51

27. Wu S.F., Liu, T.M., Lin, Y.C., Sutwu H.K.*, Juan, H.F., Chen, S.T., Shen, K.L., His, S.C. & Hsieh, S.L.* (2004) Immunomodulatory effect of decoy receptor 3 (DcR3) on the differentiation and function of bone-marrow-derived dendritic cells in non-obese diabetic mice: From regulatory mechanism to clinical implication. J. Leuk. Biol. 75, 293-306

28. Yang, C.R., Hsieh, S.L., Su, W.L., Teng, C.M., Lin, W.W (2004) Soluble decoy receptor 3 (DcR3) induces angiogenesis by neutralization of TL1A, a cytokine belonging to TNF superfamily and exhibiting angiostatic action. Cancer Research 64, 1122-1129

29. Chang, Y.C., Hsu, T.L., Lin, H.H., Chio, C.C., Chiu, A.W., Chen, N.J., Lin, C.H., Hsieh, S.L.* (2004) Modulation of macrophage differentiation and activation by decoy receptor 3. J. Leuk. Biol. 75, 486-494

30. Sung, H. H., J. H. Juang, Lin, Y. C., Kuo, C. H. Hung, J. T. Chen, A. Chang, D. M., Chang, S. Y., Hsieh, S. L., Sytwu, H. K (2004). Transgenic Expression of Decoy Receptor 3 Protects Islets from Spontaneous and Chemical-induced Autoimmune Destruction in Nonobese Diabetic Mice. J Exp Med 199(8): 1143-51

31. Lee, O.K., Kuo, T.K., Chen, W.M., Lee, K.D., Hsieh, S.L., Chen, T.H. (2004) Isolation of multi-potent mesenchymal stem cells from umbilical cord blood. Blood. 103, 1669-1675

32. Yang, C.R., Wang, J.H., Hsieh, S.L., Wang, S.M., Hsu, T.L., and Lin, W.W. (2004) Decoy Receptor 3 (DcR3) Induces Osteoclast Formation from Monocyte/Macrophage Lineage Precursor Cells. Cell Death and Differentiation 11, S97-S107

33. Wu, Y.Y., Chang, Y.C., Hsu, T.L., Hiseh, S.L., Lai, M.Z (2004) Sensitization of Cells to TRAIL-Induced Apoptosis by Decoy Receptor 3. J. Biol. Chem 279, 44211-8

34. Chen, C.C., Yang, Y.H., Lin, Y.T., Hsieh, S.L., Chiang, B.L. (2004) Soluble decoy receptor 3: Increased levels in atopic patients. J. Allergy Clin Immunol. 144, 195

35.Yang, C.R., Hsieh, S.L., Su, W.L., Teng, C.M., Lin, W.W. Soluble decoy receptor 3 (DcR3) induces angiogenesis by neutralization of TL1A, a cytokine belonging to TNF superfamily and exhibiting angiostatic action. Cancer Research 2004;3(:1122-1129. (Impact factor: 7.6160;Rank: 11/123)

36. Yang, C.R., Hsieh, S.L., Ho, F.M., Lin, W.W. (2005) Decoy Receptor 3 Increases Monocyte Adhesion to Endothelial Cells via NF-{kappa}B-Dependent Up-Regulation of Intercellular Adhesion Molecule-1, VCAM-1, and IL-8 Expression. J. Immunol. 174, 1647-56

37. Chang, Y.H., Hsieh, S.L., Chao, Y., Chou, Y.C., Lin, W.W. (2005) Proinflammatory effects of LIGHT through HVEM and LT-beta-R interactions in cultured human umbilical vein endothelial cells. J. Biomed. Sci. 12, 363-75

38. Hsu, T.L., Wu, Y.Y., Lai, M.Z., Su, W.B., Hsieh, S.L.* (2005) Attenuation of T Helper 1 Response in Decoy Receptor 3 Transgenic Mice J. Immunol. 175, 5135-45

39. J. T. Hung, J. H. Liao, Y. C. Lin, H. Y. Chang, S. F. Wu, T. H. Chang, J. T. Kung, S. L. Hsieh, H. McDevitt and H. K. Sytwu. Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from aT1 and T2 doubly transgenic non-obese diabetic mouse model. JOURNAL OF AUTOIMMUNITY 2005;25:181-192. (Impact factor: 2.4910;Rank: 53/119)

40. K. H. Chi, S. J. Liu, C. P. Li, H. P. Kuo, Y. S. Wang, Y. Chao and S. L. Hsieh. Combination of conformal radiotherapy and intratumoral injection ofadoptive dendritic cell immunotherapy in refractory hepatoma. JOURNAL OF IMMUNOTHERAPY 2005;28:129-135. (Impact factor: 3.6440;Rank: 14/74)

41. L. F. Huang, Y. K. Liu, C. A. Lu, S. L. Hsieh and S. M. Yu.. Production of human serum albumin by sugar starvation induce. TRANSGENIC RESEARCH 2005;14:569-581. (Impact factor: 2.5430;Rank: 41/139)

42. P. H. Wang, W. L. Lee, C. M. Juang, Y. H. Yang, W. H. Lo, Lai CR, S. L. Hsieh and C. C. Yuan. Altered mRNA expressions of sialyltransferases in ovarian cancer. GYNECOLOGIC ONCOLOGY 2005;99:631-639. (Impact factor: 2.2510;Rank: 12/57)

43. Su, W.B., Chang, Y.H., Lin, W.W., Hsieh, S.L.* (2006) Differential Regulation of Interleukin-8 Gene Transcription by Death Receptor 3 (DR3) and Type I TNF Receptor (TNFRI) Exp. Cell. Res. 312, 266-277

44. Chang, Y.C., Chan, Y.H., Jackson, D.G., Hsieh, S.L.* (2006) Stimulation of Cell Adhesion via Association with Cell Surface Heparan Sulfate Proteoglycans by Decoy Receptor 3. J. Immunol. 176, 173-180

You, RI, Chen, MC, Wang, HW, and Hsieh, SL* (2006) Inhibition of Lymphotoxin-beta Receptor (LT-beta R)-mediated Cell Death by Survivin-DEx3CCancer Research 66(6):3051-3061

Y. C. Chang, Y. H. Chan, D. G. Jackson and S. L. Hsieh.. The glycosaminoglycan-binding domain of decoy receptor 3 is essential forinduction of monocyte adhesion.. Journal of Immunology 2006;176:173-180. (Impact factor: 6.3870;Rank: 12/119)

47. Wei-Nan Lian, Cheng-Hung Chang, Yin-Jhen Chen, Ro-Lan Dao, Yun-Cin Luo, Jun-Yi Chien, Shie-Liang Hsieh, Chi-Hung Lin,. "Intracellular delivery can be achieved by bombarding cells or tissues with accelerated molecules or bacteria without the need for carrier particles" . EXPERIMENTAL CELL RESEARCH 2006;10:1016-1027. (Impact factor: 4.1480;Rank: 31/123)

48.W. B. Su, Y. H. Chang, W. W. Lin and S. L. Hsieh. Differential regulation of interleukin-8 gene transcription by deathreceptor 3 (DR3) and type I TNF receptor (TNFRI). EXPERIMENTAL CELL RESEARCH 2006;3(:266-277. (Impact factor: 4.1480;Rank: 31/123)

49.Shih-Jen Liu, Chih-Hsiang Lenga, Shu-pei Lien, Hsiang-Yun Chi, Chiung-Yi Huang,Chang-Ling Lin, Wei-Cheng Lian, Chi-Ju Chen, Shie-Liang Hsieh, Pele Chong. "Immunological characterizations of the nucleocapsid protein based SARS vaccine candidates" . Vaccine 2006;24(16):3100V3108. (Impact factor: 2.8220;Rank: 1/129)

50.Lu, Y. T.,Yen, C. Y.,Ho, H. C.,Chen, C. J.,Wu, M. F.,Hsieh, S. L.. Preparation and characterization of monoclonal antibody against protein TREM-like transcript-1 (TLT-1) . HYBRIDOMA 2006;1(1):20-26. (Impact factor: 0.0000;Rank: 114/119)

51.CHUN-YU WEI, YIN-HSIANG CHOU, FENG-MING HO, SHIE-LIANG HSIEH,AND WAN-WAN LIN. "Signaling Pathways of LIGHT Induced Macrophage Migration and Vascular Smooth Muscle Cell Proliferation" . JOURNAL OF CELLULAR PHYSIOLOGY 2006;209(3):735V743. (Impact factor: 4.3620;Rank: 9/75)

52. CHIH-HSIN TANG, TSUI-LING HSU,WAN-WAN LIN, MING-ZONG LAI, RONG-SEN YANG,SHIE-LIANG HSIEH* and WEN-MEI FU*. "Attenuation of bone mass and increase of osteoclast formation in decoy receptor 3 transgenic mice" . JOURNAL OF BIOLOGICAL CHEMISTRY 2006;:1074-1091. (Impact factor: 5.8540;Rank: 38/264)

53. Chen, S. J.,Chu, M. L.,Wang, C. J.,Liao, C. L.,Hsieh, S. L.,Sytwu, H. K.,Wang, C. C.. Kinetic Th1/Th2 responses of transgenic mice with bacterial meningitis induced by Haemophilus influenzae . CLINICAL SCIENCE 2006;111(4):253-263. (Impact factor: 2.6410;Rank: 24/74)

54.C.-K. How, C.-H. Chern, M.-F. Wu, L.-M. Wang, C.-I. Huang, C.-H. Lee, S.-L. Hsieh. Expression of the triggering receptor expressed on myeloid cells-1 mRNA in a heterogeneous infected population. INTERNATIONAL JOURNAL OF CLINICAL PRACTICE 2006;61(1):1-8. (Impact factor: 1.1430;Rank: 45/107)

Members of Laboratory