¡@Institute of Genetics, National Yang Ming University
The RiboLin group is applying state-of-the-art methods to design protein for practical applications. These fascinating and complex problems require contributions from disciplines that include the structure biology and molecular biology. The motivation for using the methods of domain swapping in protein engineering to design a new protein with an add-on-function arises from two principles:
(1) the correlation between the 3-D structure and function of a given protein, and
(2) the basic understanding on the protein-RNA interaction.
Withstanding these principles, we have found that a variety of useful proteins can be generated, including enzyme with greatly improved stability, the ability to carry out reactions on a new substrate. Another interest of The RiboLin group is the study of the molecular trafficking of eukaryotic ribosome components. This includes the understanding of how eukaryotic ribosomal components involve in the binding of the endoplasmic reticulum and the nuclear transportation of ribosomal components.
Structure and Function of Eukaryotic ribosome
Protein - ribonucleic acid Interaction
Lin, A., Huang, K-C., Hwu, L., and Tzean, S. S. Production of ribotoxins among Aspergillus species and its related fungi. Toxicon, 33, 105-110.(1995)
Lin, A. Dynamics of Ribotoxin: (1) Characterization of Liver-specific Immunotoxin 9B2-a. Proceedings of the 11th International Conference on Advanced Science and Technology, Vol. 11, p110-118.(1995)
Cheung, Jin-I, Yuh-Ru Wang, and Lin, A. Mode Action of Dimeric Form of a-Sarcin. FEBS Lett., 386, 60-64. (1996)
K-C. Huang, Y-Y. Hwang, L. Hwu, and Lin, A. Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus. Toxicon 35, 383-392.(1997)
Lin, E., S. R. Liu, and Lin, A. (1999) Biochemical analysis of a 5S rRNA-associated sub-particle from trypsinized eukarotic ribosomes. J. Biochem, 125, 1029-1033.
Chueing, Jin-I,, and Lin, A. (1999) Probing surface structure of sarcin domain on ribosomes of Escherichia coli. by complementary oligo DNAs and ribosome-inactivating protein. J. Biomed.Sci. 6, 227-284.
Hwu Luen, Kuan-Chun Huang, Dow-Tien Chen, and Lin, A. (2000) The action mode of the ribosome-inactivating protein a-sarcin. J. Biomed. Sci. 7, 420-428.
Chang, Shu-Nu, Lin, Chi-Hung, and Lin, A. (2000) An acidic amino acid cluster regulates the nucleolar localization and ribosome assembly of human ribosomal protein L22. FEBS Lett. 484,22-28.
Lin, A., Chiou-Jau Cho, and TzeanZ, S. S..(2000) Nucleotide sequence of ribostoxin gene (sar gene) from strains of Penicillium and their actions to inactivate ribosomes. Bot.Bul. (in press).
Lin, Elong, Lin, Sue-Wen.,and Lin, A. (2001) Eukaryotic 5S rRNA participation in the co-translational formation of a 5S rRNA ribonucleic acid-protein complex during the transaltion of ribosomal proein L5. submitted to Nucleic Acids Res.