Publications of CWC lab 1991-2008

Tseng H-C, Chen CW (1991) A cloned ompR-like gene of Streptomyces lividans 66 suppresses defective melC1, a putative copper-transfer gene. Molecular Microbiology 5: 1187-1196.

Chen CW, Yu T-W, Chung H-M, Chou CF (1992) Discovery and characterization of a new transposable element, Tn4811, in Streptomyces lividans 66. Journal of Bacteriology 174: 7762-7769. [Abstract]

Chou C-F, Chen CW (1992) argJ mutations are highly inducible by ethidium bromide in proB strains of Streptomyces lividans: Implication of pathway interactions. Biochemical Biophysical Research Communications 189: 1101-1109.

Yu T-W, Chen CW (1993) The unstable melC operon of Streptomyces antibioticus is co-deleted with a Tn4811-homologous locus. Journal of Bacteriology 175: 1847-1852.

Chen CW, Yu TW, Lin Y-S, Kieser HM, Hopwood DA. (1993) The conjugative plasmid SLP2 of Streptomyces lividans is a 50-kb linear DNA molecule. Molecular Microbiology 7: 925-932.

Lin Y-S, Kieser HM, Hopwood DA, Chen CW (1993) The chromosomal DNA of Streptomyces lividans 66 is linear. Molecular Microbiolog 10: 923-933.

Chen CW, Lin Y-S, Yang Y-L, Tsou M-F, Chang H-M, Kieser HM, Hopwood DA (1994) The linear chromosomes of Streptomyces: structure and dynamics. Actinomycetologica 8: 103-112.

Chen CW (1995) The unstable end of the Streptomyces linear chromosomes: A nuisance without cures? Trends in Biotechnology 13:157-160. [Manuscript]

Lee L-F, Huang Y-J, Chen CW (1996) Isolation and characterization of two classes of ethidium bromide-resistant mutants of Streptomyces lividans 66. Microbiology 140: 1041-1047.

Chen CW (1996) Complications and implications of linear bacterial chromosomes. Trends in Genetics 12: 192-196. [Manuscript]

Chang H-M, Shieh Y-T, Chen M-Y, Bibb MJ, Chen CW (1966) The cutRS signal transduction system of Streptomyces lividans represses the biosynthesis of a polyketide antibiotic actinorhodin. Molecular Microbiology 21: 1075-1085. [Abstract]

Lin Y-S, Chen CW (1997) Instability of artificailly circularised chromosomes of Streptomyces lividans. Molecular Microbiology 26: 709-719 [Abstract]

Chen CW (1997) Threads of evidence - On the trail to linear bacterial chromosomes. SAJ News. 1-29 [Read the manuscript]

Huang C-H, Chen CW (1998) The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures. Molecular Microbiology 28: 905-916. [Abstract]

Lee L-F, Chen CW (1998) Multidrug resistance gene in Streptomyces. Actinomycetologica 12: 148-152

Wang S-J, Chang H-M, Lin Y-S, Huang C-H, Chen CW (1999) Streptomyces genomes: Circular genetic maps from the linear chromosomes. Microbiology145: 2209-2220 [Read the manuscript]

Bey S-J, Tsou M-F, Huang C-H, Yang C-C, Chen CW (2000) The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid. Microbiology 146: 911-922 [Abstract]

Lee LF, Yeh SH, Chen CW. (2001) Construction and synchronization of dnaA temperature-sensitive mutants of Streptomyces.Journal of Bacteriology 184:1214-1218. [PubMed]

Yang CC, Huang CH, Li CY, Tsay YG, Lee SC, Chen CW. (2002) The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins. (2002) Molecular Microbiology 43 :297-305. [PubMed]

Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thomson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D, Bateman A, Brown S, Chandra G, Chen CW, Collins M, Cronin A, Fraser A, Goble A, Hidalgo J, Hornsby T, Howarth S, Huang CH, Kieser T, Larke L, Murphy L, Oliver K, O'Neil S, Rabbinowitsch E, Rajandream MA, Rutherford K, Rutter S, Seeger K, Saunders D, Sharp S, Squares R, Squares S, Taylor K, Warren T, Wietzorrek A, Woodward J, Barrell BG, Parkhill J, Hopwood DA.(2002) Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141-147.[PubMed]

 Lee, L.F., Yeh, S.H., and Chen CW. (2002) Construction and synchronization of dnaA temperature-sensitive mutants of Streptomyces. J Bacteriol 184: 1214-1218. [PubMed]

Yang, C.-C., Huang, C.-H., Li, C.-Y., Tsay, Y.-G., Lee, S.-C., and Chen CW. (2002) The terminal proteins of linear Streptomyces chromosomes and plasmids: A novel class of replication priming proteins. Mol Microbiol 43: 297-305. [PubMed]

 Huang, C.H., Chen, C.Y., Tsai, H.H., Chen, C., Lin, Y.S., and Chen CW. (2003) Linear plasmid SLP2 of Streptomyces lividans is a composite replicon. Mol Microbiol 47: 1563-1576.[PubMed]

Lee, L.F., Huang, Y.J., and Chen CW. (2003) Repressed multidrug resistance genes in Streptomyces lividans. Arch Microbiol 180: 176-184. [PubMed]

Bentley, S.D., Brown, S., Murphy, L.D., Harris, D.E., Quail, M.A., Parkhill, J., Barrell, B.G., McCormick, J.R., Santamaria, R.I., Losick, R., Yamasaki, M., Kinashi, H., Chen CW., Chandra, G., Jakimowicz, D., Kieser, H.M., Kieser, T., and Chater, K.F. (2004) SCP1, a 356,023 base pair linear plasmid adapted to the ecology and developmental biology of its host, Streptomyces coelicolor A3(2). Mol Microbiol 51: 1615-1628.[PubMed]

Weaver, D., Karoonuthaisiri, N., Tsai, H.H., Huang, C.H., Ho, M.L., Gai, S., Patel, K.G., Huang, J., Cohen, S.N., Hopwood, D.A., Chen CW., and Kao, C.M. (2004) Genome plasticity in Streptomyces: Identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome. Mol Microbiol 51: 1530-1550. [PubMed]

 Chaconas, G., and Chen CW. (2005) Linear chromosomes in bacteria: no longer going around in circles. In The Bacterial Chromosome. Higgins, N.P. (ed). Washington, D. C.: American Society for Microbiology, pp. 525-539.

Huang, T.W., and Chen CW. (2006) A recA null mutation may be generated in Streptomyces coelicolor. J Bacteriol 188: 6771-6779.[PubMed]

Tseng, S.F., Huang, T.W.,Chen CW., Chern, M.K., Tam, M.F., and Teng, S.C. (2006) ShyA, a membrane protein for proper septation of hyphae in Streptomyces. Biochem Biophys Res Commun 343: 369-377. [PubMed]

Yang, C.-C., Chen, Y.-H., Tsai, H.-H., Huang, C.-H., Huang, T.W., andChen CW. (2006) In vitro deoxynucleotidylation of terminal prortein of Streptomyces linear chromosomes. Applied and Environmental Microbiology, Dec. 2006, p.7959-7961. [PubMed]

Huang C.-H., Tsai, H.-H., Tsay, Y.-G., Chien, Y.-N., Wang, S.-L., Cheng, M.-Y., Ke, C.-H., Chen CW. (2007) The telomere system of the Streptomyces linear plasmid SCP1 represents a novel class. Mol. Microbioi. 63: 1710-1718. [PubMed]

Lee, L.-L., Chen, Y.-J., Kirby, R., Chen, C., Chen CW. (2007) A multidrug efflux system is involved in colony growth in Streptomyces lividans. Microbiol. 153: 924–934.[PubMed]

Chi Chen, Chen CW. (2007) Quantitative analysis of mutation and selection pressures on base composition skews in bacterial chromosomes. BMC Genomics. 8:286[PubMed]

Tzu-Wen Huang, Chen CW. (2008) DNA Polymerase I Is Not Required for Replication of LinearChromosomes in Streptomyces. Journal of bacteriology  190:755-758.[PubMed]

Tsai, H.-H., Huang, C.-H., Lin, A. M.,Chen CW. (2008) Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei. Nucleic Acid Res. In press.