Yan-Hwa Wu Lee 

    PhD. Biochemistry, University of Tennessee



     Virus often targets critical regulatory events in host cells.  The effects of viruses on the host cells can be mediated by addition or substitution of virus-specific macromolecules to a cellular complex. Alternatively, the virus may cause a disassembly or rearrangement of a host-cell complex, or lead to the assembly of a new infected cell-specific complex. Thus, knowledge of the processes subverted by viruses has often highlighted the mechanisms of viral pathogenesis. The main goal of our study is to elaborate the types of interactions between hepatitis virus-encoded macromolecules and the host cells, which may define the ultimate outcome of a virus infection.



  1. Chen, C.M., You, L.R., Hwang, L.H., and Lee, Y.H.W.  (1997). Direct interaction of hepatitis C virus core protein with the cellular lymphotoxin-b receptor modulates the signal pathway of the lymphotoxin-b receptor. J. Virology 71, 9417-9426.
  2. Yeh, T.S., and Lee, Y.H.W. (1998). Assembly of hepatitis delta virus particles: package of multimeric HDV genomic RNA and role of phosphorylation. Virology 249, 12-20.
  3. You, L.R., Chen, C.M., and Lee, Y.H.W. (1999). The hepatitis C virus core protein modulates the NF-kB signal pathway triggering by lymphotoxin-b receptor ligands and tumor necrosis factor-a.  J. Virology 73, 1672-1681.  
  4. You, L.R., Chen, C.M., Yeh, T.S., Tsai, T.Y., Mai, R.T., Lin, C.H., and Lee, Y.H.W. (1999). Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virology 73, 2841-2853.
  5. Chung, Y.L., Lee, Y.H.W., Yen, S.H., and Chi, K.W. (2000). A novel approach for nasopharyngeal carcinoma treatment uses phenylbutyrate as a protein kinase c modulator: implications for radiosensitization and EBV-targeted theraphy. Clin. Cancer Res. 6, 1452-1458.
  6. Huang, W.H. Yung, B.Y.M., Syu, W.J., and Lee, Y.H.W. (2001). The nucleolar phosphoprotein B23 interacts with hepatitis delta antigen and modulates the hepatitis delta virus RNA replication. J. Biol. Chem. 276, 25166-25175.
  7. Chen, S.Y., Kao, C.F., Chen, C.M., Shih, C.M., Hsu, M.J., Chao, C.H. C., Wang, S.H., You, L.R., and Lee, Y.H.W. (2003). Mechanisms for inhibition of hepatitis B virus gene expression and replication by hepatitis C virus core protein. J. Biol. Chem. 278, 591-607.
  8. Kao, C.F., Chen, S.Y., Chen, J.Y., Lee, Y.H.W. (2004) Modulation of p53 transcription regulatory activity and posttranslational modification by hepatitis C virus core protein. Oncogene 23, 2472-2483
  9. Kao, C.F., Chen, S.Y., Lee, Y.H.W. (2004) Activation of RNA polymerase I transcription by hepatitis C virus core protein. J. Biomed. Sci. 11, 72-94.
  10. Cheng, P.L., Chang, M.H., Chao, C.H., Lee, Y.H.W. (2004) Hepatitis C viral proteins interact with Smad3 and differentially regulate the TGF-b/Smad3-mediated transcriptional activation. Oncogene 23, 7821-7838.
  11. Mai, R.T., Yeh, T.S., Huang, H.H.,  Sun, S.K. and Lee, Y.H.W.  (2006) Hepatitis C virus core protein recruits nucleolar phosphoprotein B23 and coactivator p300 to relieve the repression effect of transcription factor YY1 on B23 gene expression. Oncogene 25, 448-462.
  12. Chang, P.C., Chi, C.W., Chau, G.Y., Li, F.Y., Tsai, Y.H., Wu, J.C., and Lee, Y.H.W. (2006) DDX3. a DEAD box RNA helicase, is deregulated in hepatitis virus associated hepatocellular carcinoma and is involved in cell growth control. Oncogene 25, 1991-2003.
  13. Chao, C.H., Chen, C.M., Cheng, P.L., Shih, J.W., Tsou, A.P., and Lee, Y.H.W. (2006) DDX3, A DEAD box RNA helicase with tumor growth-suppressive property and transcriptional regulation activity of the p21waf1/cip1 promoter, is a candidate tumor suppressor. Cancer Research 66(13):6579-6588
  14. Shih, J.-W., T.-Y. Tsai, and Y.-H. W. Lee*. Candidate tumor suppressor DDX3 RNA helicase  specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein. Oncogene (in press).
  15. Huang, W.H., Mai, R.T., and Lee, Y.H.W. The transcription factor YY1 and its associated acetyl-transferases CBP and p300 interact with hepatitis delta antigens and modulates the hepatitis delta virus RNA replication (manuscript in preparation).