Yeou-Guang Tsay

PhD (Biochemistry), Michigan State University

MD (Medicine), National Taiwan University


      The primary interest of our laboratory is to understand the biological functions of two classes of protein structures, namely posttranslational modifications and quaternary structures (or protein complexes).  The metabolism of these protein structures are long believed  to be the fundamental mechanisms that modulate proteins functions in many important biological processes.  However, for most protein molecules, how their activities are governed by these mechanisms remains poorly understood.  In order to facilitate the researches on these structures, our laboratory is dedicated to the acquisition and organization of the information about posttranslational modifications and quaternary structures, chiefly through development and application of novel mass spectrometry-based methodologies. The specific goals of our ongoing projects are (1) to establish posttranslational modification databases of human proteomes via our comprehensive posttranslational modification mapping procedure and  (2) to develop the technology that can efficiently identify and quantify the quaternary structures in a proteome.


  1. Tsay YG, Cheng CC, Hu ST (2005)  Identification of the -1 translational frameshift sites using liquid chromatography-tandem mass spectrometry (2005) Anal. Biochem.  339(1):83-93 

  2. Lin SK, Chang MC, Tsay YG, Lur SH (2005)  Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics 5(8):2140-2156.

  3. Chang YW, Chen SC, Cheng EC, Ko YP, Lin YC, Kao YR, Tsay YG, Yang PC, Wu CW, Roffler SR. (2005)  CD13 (aminopeptidase N) can associate with tumor-associated antigen L6 and enhance the motility of human lung cancer cells.  Int. J. Cancer.  In press.

  4. Lai LP, Lin JL, Lin CS, Yeh HM, Tsay YG, Lee CF, Lee HH, Chang ZF, Hwang JJ, Su MJ, Tseng YZ, Huang SK. (2004)  Functional genomic study on atrial fibrillation using cDNA microarray and two-dimensional protein electrophoresis techniques and identification of the myosin regulatory light chain isoform reprogramming in atrial fibrillation. J Cardiovasc Electrophysiol. 15(2):214-23.

  5.  Mu JJ, Tsay YG, Juan LJ, Fu TF, Huang WH, Chen DS, Chen PJ. (2004)  The small delta antigen of hepatitis delta virus is an acetylated protein and acetylation of lysine 72 may influence its cellular localization and viral RNA synthesis. Virology. 319(1):60-70.

  6. Hsu RB, Tsay YG, Wang SS, Chu SH. (2003)  Management of aortic aneurysm infected with Salmonella. Br J Surg. 90(9):1080-4.

  7. Hsu RB, Tsay YG, Lee CM, Chen RJ, Wang SS, Chu SH. (2003)  Soluble inflammatory markers in coronary sinus and peripheral blood of heart transplant recipients. Clin Transplant. 17(4):331-7.

  8. Wang YH, Tsay YG, Tan BC, Lo WY, Lee SC. (2003)  Identification and characterization of a novel p300-mediated p53 acetylation site, lysine 305. J Biol Chem. 278(28):25568-76.

  9. Hsu RB, Tsay YG, Chen RJ, Chu SH. (2003)  Risk factors for primary bacteremia and endovascular infection in patients without acquired immunodeficiency syndrome who have nontyphoid salmonellosis. Clin Infect Dis. 36(7):829-34.

  10. Hsu RB, Tsay YG, Wang SS, Chu SH. (2002)  Surgical treatment for primary infected aneurysm of the descending thoracic aorta, abdominal aorta, and iliac arteries. J Vasc Surg. 2002 Oct;36(4):746-50.

  11. Lin WC, Shen BJ, Tsay YG, Yen HC, Lee SC, Chang CJ. (2002)  Transcriptional activation of C/EBPbeta gene by c-Jun and ATF2. DNA Cell Biol. 21(8):551-60.

  12. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ. (2002)  An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K. J Biol Chem. 277(43):40403-9.

  13.  Chiu CM, Tsay YG, Chang CJ, Lee SC. (2002)  Nopp140 is a mediator of the protein kinase A signaling pathway that activates the acute phase response alpha1-acid glycoprotein gene. J Biol Chem. 277(42):39102-11.

  14. Chen CW, Tsay YG, Wu HL, Lee CH, Chen DS, Chen PJ. (2002)  The double-stranded RNA-activated kinase, PKR, can phosphorylate hepatitis D virus small delta antigen at functional serine and threonine residues. J Biol Chem. 277(36):33058-67.

  15. Yang CC, Huang CH, Li CY, Tsay YG, Lee SC, Chen CW. (2002)  The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins. Mol Microbiol. 43(2):297-305.

  16. Chia JS, Chang LY, Shun CT, Chang YY, Tsay YG, Chen JY. (2001)  A 60-kilodalton immunodominant glycoprotein is essential for cell wall integrity and the maintenance of cell shape in Streptococcus mutans. Infect Immun. 69(11):6987-98. Erratum in: Infect Immun 70(3):1665.

  17. Tsay YG, Wang YH, Chiu CM, Shen BJ, Lee SC. (2000)  A strategy for identification and quantitation of phosphopeptides by liquid chromatography/tandem mass spectrometry. Anal Biochem. 287(1):55-64.

  18. Tsay YG, Lin NY, Voss PG, Patterson RJ, Wang JL. (1999)  Export of galectin-3 from nuclei of digitonin-permeabilized mouse 3T3 fibroblasts. Exp Cell Res. 252(2):250-61.