Ying-Chieh Tsai

    PhD in Agriculture, Tokyo University


  1. Scientific aromatherapy and functional studies of essential oils from Chinese herbs
  2. Structural and functional studies of industrial enzymes (expandase, aminoacylase, saccharide oxidase)
  3. Inhibitors of sialyltransferase and fucosyltransferase and their anti-cancer, anti-infection activities
  4. Plant-derived lactic acid bacteria
  5. Wound healing mechanism of sulbogin and other Chinese herbs.

Publications (2001-2006)

  1. Wu, C.Y., Hsu, C.C., Chen, S.T., and Tsai, Y.C.* (2001) Soyasaponin I, a potent and specific sialyltransferase inhibitor. Biochem. Biophys. Res. Comm. 284:466-469.

  2. Wang, P.H., Li, Y. F., Juang, C.M., Lee, Y.R., Chao, H.T., Tsai, Y.C., and Yuan, C.C. (2001) Altered mRNA expression of sialyltransferase in squamous cell carcinomas of the cervix. Gynecologic Oncology 83:121–127.

  3. Su, J.H., Chuang, Y.C., Tsai, Y.C., and Chang, M. C. (2001) Cloning and characterization of a blue fluorescent protein from Vibrio vulnificus. Biochem. Biophys. Res. Comm. 287:357-365.

  4. Yeh CM*, Yang MC, Tsai, Y.C. (2002) Application potency of engineered G159 mutants on P1 substrate pocket of subtilisin YaB as improved meat tenderizers. J. Agric. Food Chem. 50:6199-204.

  5. Lin, P.H, Su S.C., Tsai Y.C. and Lee, C.Y. (2002) Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N-acyl-D-amino acid amidohydrolase responsible for D-amino acid production. Eur. J. Biochem. 269:4868-4878.

  6. Chien, H.C.R., Lin L.L., Chao, S.H., Chen, C.C., Wang, W.C., Shaw, C.Y., Tsai, Y.C. Hu, H.Y., and Hsu, W.H. (2002) Purification, characterization, and genetic analysis of a leucine aminopeptidase from Aspergillus sojae. Biochim. Biophys. Acta 1576:119–126.

  7. Chang, Y.S., Liaw, S.H., Mei, H.C., Hsu, C.C., Wu, C.Y., and Tsai, Y.C.* (2002) Mutational analysis of the autoprocessing site of subtilisin YaB-G124A. Biochem. Biophys. Res. Comm. 291:165-169.

  8. Hsu, C.S., Lai, W.L., Chang, W.W., Liaw, S.H.,* and Tsai, Y.C.* (2002) Structure-based mutational analysis of the D-aminoacylase from Alcaligenes faecalis DA1. Protein Sci. 11:2545-2550.

  9. Hsu CS, Chen SJ, Tsai YC, Lin TW, Liaw SH, Wang AH. (2002) Crystallization and preliminary crystallographic analysis of a D-aminoacylase from Alcaligenes faecalis DA1. Acta. Crystallogr. D Biol. Crystallogr. 58:1482-3.

  10. Chen, C.H., Shaw, C.Y., Chen, C.C., and Tsai, Y.C.* (2002) 2,3,4-Trimethyl-5,7-dihydroxy-2,3- dihydrobenzofuran, a novel antioxidant, from Penicillium citrinum F5. J. Nat. Prod. 65:740-741.

  11. Wang, P.H., Li, Y.F., Juang, C.M., Lee, Y.R., Chao, H.T., Ng, H.T., Tsai, Y.C.*, and Yuan, C. C.* (2002) Expression of sialyltransferase family members in cervix squamous cell carcinoma correlates with lymph node metastasis. Gynecol. Oncol. 86:45-52.

  12. Wang PH., Lo WL, Hsu CC., Lin TW, Lee WL, Wu CY, Yuan CC, and Tsai YC*. (2002) Different enzyme activities of sialyltransferase in gynecological cancer cell lines. Eur. J. Gynecol. Oncol. 23:221-226.

  13. Chen CC, Lee WL, Tsai YC, Yuang CC, Ng HT, and Wang PH*. (2002) Sialyltransferase family members and cervix squamous cell carcinoma. Eur. J. Gynaecol. Oncol. 23:514-51.

  14. Yeh CM*, Young MJ, and Tsai YC. (2002) A simplified purification method for the recombinant subtilisin YaB. Taiwan Journal of Agricultural Chemistry and Food Science; 40:45-54.

  15. Wei, C.L., Yan,g Y.B., Wang, W.C., Liu, W.C., Hsu, J.S., and Tsai, Y.C.* (2003) Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G. Appl. Environ. Microbiol. 69:2306-2312.

  16. Liaw, S.H, Chen, S.J, Ko, T.P, Hsu, C.S, Chen, C.J, Wang, A.H, and Tsai, Y.C.* (2003) Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1. A novel subset of amidohydrolases and insights into the enzyme mechanism. J. Biol. Chem. 278:4957-62.

  17. Mai, L.M., Lin, C.Y., Chen, C.Y., and Tsai, Y.C.* (2003) Synergistic effect of bismuth subgallate and borneol, the major components of Sulbogin, on the healing of skin wound. Biomaterials. 24:3005-3012.

  18. Mei, H.C., Li, Y.F., Hsu, C.C., Tsai, Y.C.*, and Takagi, H.* (2003) Conversion of the cleavage specificity of subtilisin YaB on oxidized insulin chains to an elastase-like specificity by replacement of Gly124 with Ala. Biosci. Biotechnol. Biochem. 67:1601-1604.

  19. Shaw, C.Y., Chen, C.H., Hsu, C.C., Chen, C.C., and Tsai, Y.C.* (2003) Antioxidant properties of scopoletin isolated from Sinomonium acutum. Phytoth. Res. 17:823-825.

  20. Wang, P.H.*, Lee, W.L., Lee, Y.R., Juang,C.M., Chen, Y.J., Chao, H.T., Tsai, Y.C. and Yuan, C.C. (2003) Enhanced expression of a-sialyltransferase ST6Gal I in cervical squamous cell carcinoma. Gynecol. Oncol. 89:395-401.

  21. Lin, C.Y. , Shen, Y.H. , Wu, S.H. , Lin, C.H. , Hwang, S.M. and Tsai, Y.C.* (2004) Effect of bismuth subgallate on nitric oxide and prostaglandin E2 production by macrophages. Biochem. Biophys. Res. Commun. 315: 830-835.

  22. Lai, W.L., Chou, L.Y., Ting, C.Y., Kirby, R., Tsai, Y.C., Wang, A.H.J., and Liaw, S.H.* (2004) The functional role of the binuclear metal center in D-aminoacylase one-metal activation and second-metal attenuation. J. Biol. Chem. 279:13962-7.

  23. Huang, H.Y., Chang, C.K., Tso, T.K., Huang, J.J., Chang, W.W., and Tsai, Y.C.* (2004) Antioxidant activities of various fruits and vegetable produced in Taiwan. Inter. J. Food Sci. Nutr. 55:423-429.

  24. Hsu, J.S., Yang, Y.B., Deng, C.H., Wei, C.L., Liaw, S.H., and Tsai, Y.C.* (2004) Family shuffling of expandase genes to enhance substrate specificity for penicillin G. Appl. Environ. Microbiol. 70: 6257-6263.

  25. Lin, Y.H., Li, Y.F., Huang, M.C. and Tsai, Y.C.*(2004) Intracellular expression of Vitreoscilla haemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture. Biotechnol. Lett. 26: 1067-1072.

  26. Huang, H.Y, Huang, J.J, Tso, T.K, Tsai, Y.C, Chang, C.K. (2004) Antioxidant and angiotension-converting enzyme inhibition capacities of various parts of Benincasa hispida (wax gourd). Nahrung. 48:230-3.

  27. Hsu, C.C., Lin, T.W., Chang, W.W., Lo, W.H., Wang, P.H., and Tsai, Y.C.* (2005) Soyasaponin I modified invasive behavior of cancer by changing cell surface sialic acids. Gynecol. Oncol. 96:415-422.

  28. Lin, T.W. ,Chang, W.W., Chen, C.C. and Tsai, Y.C.* (2005) Stachybotrydial, a potent inhibitor of fucosyltransferase and sialyltransferase. Biochem. Biophys. Res. Commun. 331:953-7

  29. Huang C. H., Lai W. L., Lee, M. H., Chen C. J., Tsai, Y. C.* and Liaw, S. H.* (2005) Crystal structure of Acremonium strictum glucooligosaccharide oxidase. A novel flavinylation of 6-S-cysteinyl, 8a-N1-histidyl FAD. J. Biol. Chem. 280:38831-38838.

  30. Wei, C.L., Yang, Y.B., Deng, C.H., Liu, W.C., Hsu, J.S., Lin, Y.C., Liaw, S.H., and Tsai, Y.C.* (2005) Directed evolution of Streptomyces clavuligerus Deacetoxy-cephalosporin C synthase for enhancement of Penicillin G expansion. Appl. Environ. Microbiol. 71:8873-8880

  31. Lee, M. H., Lai, W. L., Lin, S. F., Hsu, C. S., Liaw, S. H. and Tsai, Y. C.* (2005) Structural characterization of glucooligosaccharide oxidase from Acremonium strictum. Appl. Environ. Microbiol. 71:8881-8887

  32. Lee, M. H., Lai, W. L., Lin, S. F., Liu, Y., Hsu, Y. H. and Tsai, Y. C.*(2006) Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae.  Enzyme Microbiol. Tech. 39:85-91

  33. Chao, S. H., Cheng, T.H., Shaw, C.Y., Lee, M.H., Hsu, Y.H., and Tsai, Y.C.* (2006) Characterization of a novel PepF-like oligopeptidase that is secreted from Bacillus amyloliquefaciens 23-7A. Appl. Environ. Microbiol. 72:968-971

  34. Hsu, H.C., Yang, W.C., Tsai W.J., Chen, C.C., Huang, H.Y., and Tsai, Y.C..(2006) a-Bulnesene, a novel PAF receptor antagonist isolated from Pogostemon cablin. Biochemical and biophysical research communications 345:1033-38.

  35. Hui-Chun Hsu, Wen-Chia Yang, Wei-Jern Tsai, Chien-Chih Chen, Takashi Watanabe and Ying-Chieh Tsai. (2006) á-Bulnesene, a PAF inhibitor isolated from the essential oil of Pogostemon cablin. Fitoterapia (in press)

  36. Chang, W. W., Yu, C. Y., Lin, T. W., Wang P. H., Tsai, Y. C.. (2006)Soyasaponin I decreases the expression of a2,3-linked sialic acid on the cell surface and suppresses the metastatic potential of B16F10 melanoma cells. Biochemical and biophysical research communications 341:614-619.