PhD. Chemistry, University of California, San DiegoResearch
Structural biology study may provide detailed molecular and atomic level structural information of biomolecules to correlate with their biological functions in physiological states and disturbances in disease states. In our disease-oriented study, we proposed to employ heteronuclear multi-dimensional NMR spectroscopy to determine the structure of human apolipoprotein E (apoE) and b-amyloid peptide (Ab) which are two key biomacromolecules in the pathogenesis of atherosclerosis and Alzheimer’s disease. Structural determination of protein phosphatase inhibitors was also studied by NMR spectroscopy as to reveal the molecular mechanism of phosphorylation and activation to some extent from the structural point of view.
Huang, H.B., Chen, Y.C., Tsai, L.H., Wang, H.C., Lin, F.M., Horiuchi, A., Greengard, P., Nairn, A.C., Shiao, M.S. and Lin, T.H. (2000), "Letter to the Editor: Backbone 1H, 15N, and 13C resonance assignments of inhibitor-2 - a protein inhibitor of protein phosphatase-1", J. Biomol. NMR, 17, 359-360.
Huang, H.B., Chen, Y.C., Horiuchi, A., Tsai, L.H., Liu, H.T., Chyan, C.L., Hsieh, M.J., Liu, C.K., Lin, F.M., Greengard, P., Nairn, A. C., Shiao, M.-S. and Lin, T.H. (2001), "Letter to the Editor: Backbone 1H, 15N, and 13C resonance assignments of ARPP-19", J. Biomol. NMR, 19, 383-384.
Chyan, C.L., Tang, T.C., Chen, Y.C., Liu, H.T., Lin, F.M., Liu, C.K., Hsieh, M.J., Shiao, M. S., Huang, H.B. and Lin, T.H. (2001), “Backbone 1H, 15N, and 13C resonance assignments of inhibitor-1 – a Protein Inhibitor of Protein Phosphatase-1”, J. Biomol. NMR, 21, 287-288.
Liu, H.T., Lin, T.H., Kuo, H.C., Chen, Y.-C., Tsay, H.J., Jeng, H.H., Tsai, P.C., Shie, F.K., Chen, J.H. and Huang, H.B. (2002), "Identification of the Alternative Splice Products Encoded by the Human Protein Phosphatase Inhibitor -1 Gene", Biochem. Biophy. Res. Commun., 291, 1293-1296.
Lin, T.H., Chia, C.M., Hsiao, J.C., Chang, W., Ku, C.C., Hung, S.C. and Tzou, D.L. M. (2002), "Structural Analysis of the Extracellular Domain of Vaccinia Virus Envelope Protein, A27L, by NMR and CD Spectroscopy", J. Biol. Chem., 277, 20949-20959.
Lin, T.H., Chen, Y.C, Chyan, C.L., Tsay, L.H., Tang, T.C., Jeng, H.H., Lin, F.M. and Huang, H.B. (2003), "Phosphorylation by glycogen synthase kinase of inhibitor-2 does not change its structure in free state", FEBS Lett., 554, 253-256.
Lin, T.H., Huang, Y.C., Chin, M.L., Chen, Y.C., Jeng, H.H., Lin, F.M., Shiao, M.S., Horiuchi, A., Greengard, P., Nairn, A.C. and Huang, H.B. (2004), "1H, 15N, and 13C resonance Assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000) – a Protein Inhibitor of Protein Phosphatase-1", J. Biomol. NMR, 28, 413-414.
Lou, B.-S., Lin, T.-H and Lo. C.-Z (2004), "The conformation and movement of Na+ channel inactivation gate peptide in linker between domain III and IV during inactivation by NMR spectroscopy and molecular modeling study", J. Peptide Res., 63, 313-323.
Lin, T.H, Huang, J.W., Huang, H.B., Chen, Y.C., Liu, C.Y., Lo, C.J., Tang, T.C.& Chyan, C.L. (2004), "Letter to the Editor: 1H, 15N, and 13C resonance assignments of calmodulin complexed with the calmodulin-binding domain of calcineurin", J. Biomol. NMR, 29, 531-532.
Chou, C.Y., Lin, Y.L. Huang, Y.C., Sheu, S.Y., Lin, T.H., Tsay, H.J., Chang, G.G. and Shiao, M.S. (2005), "Structural Variation in Human Apolipoprotein E3 and E4: Secondary Structure, Tertiary Structure, and Size Distribution", Biophys. J., 88, 455-466.
Lin, T.H., Huang, H.B., Wei, H.A., Shiao, S.H., Chen, Y.C. (2005), "The effect of temperature and lipid on the conformational transition of gramicidin A in lipid vesicles", Biopolymers, Published Online: 11 Mar 2005; DOI: 10.1002/bip.20269.
Yu Ho, Jye-Chian Hsiao, Min-Hsiang Yang, Che-Sheng Chung, Yu-Chang Peng, Ta-Hsien Lin, Wen Chang and Der-Lii M. Tzou. (2005) The Oligomeric Structure of Vaccinia Viral Envelope Protein A27L is Essential for Binding to Heparin and Heparan Sulfates on Cell Surfaces: A Structural and Functional Approach Using Site-specific Mutagenesis. J. Mol. Biolo. 2005;349:1060-1071.
Ta-Hsien Lin, Pai-Chi Tsai, Hsin-Tzu Liu, Yi-Chen Chen, Lan-Hsin Wang, Fu-Kai Hsieh and Hsien-Bin Huang. (2005) Characterization of the Protein Phosphatase 1–binding Motifs of Inhibitor-2 and DARPP-32 by Surface Plasmon Resonance. J. Biochem. 138:697-700.
Lou, B.-S., Lin, T.-H and Lo. C.-Z. (2005) The interactions of phenytoin and its binding site in DI-S6 segment of Na+ channel voltage-gated peptide by NMR spectroscopy and molecular modeling study. J. Peptide Res. 66:27-38.
Chia-Lin Chyan, Po-Chung Huang, Tai-Hsien Lin, Jian-Wen Huang, S. S. Lin, Hsien-bin Huang and Yi-Chen Chen. (2005) Purification, crystallization and preliminary crystallographic studies of a Camodulin-OLFp molecule. Acta Cryst., F 61:785-787
Y.R. Chen, H.B. Huang, C.L. Chyan, M.S. Shiao, T.H. Lin and Y.C. Chen. (2006) The Effect of Ab Conformation on the Metal Affinity and Aggregation Mechanism Studied by Circular Dichroism Spectroscopy. J. Biochem.139:733-740.