PhD. Biochemistry , National Taiwan University
The major interest of our laboratory is the study of endogenous (physiological) and exogenous (dietary) factors that may protect human from developing atherosclerosis. The basic underlying cause of atherosclerosis is LDL peroxidation within intravascular cells. Our previous study has implicated that b2-glycoprotein I (b2-GPI, also known as apolipoprotein H) may inhibit LDL oxidation and lipid accumulation in macrophages. The regulation of b2-GPI expression under oxidative stress was under investigation both at transcription and translation levels. We are also working on a series of studies to identify the dietary components effective in inhibiting LDL oxidation. A recent line of study delineated the relationship between n-3 polyunsaturated fatty acid (n-3 PUFA), peroxisome proliferators-activated receptors (PPARs), and atherogenesis. We have established vascular cell model (macrophages and smooth muscle cells) and atherosclerotic animal model (apoE knock out mice) for exploring the control of progression and regression of atherosclerosis.
Chiang, A.N., Wu, H.L.,Yeh, H.I., Chu, C.S., Lin,H.C., and Lee, W.C. Antioxidant Effects of Black Rice Extract through the Induction of Superoxide Dismutase and Catalase Activities. Lipids 41(8): 797-803.
Chen, C.J., Ou, Y.C., Lin, S.Y., Liao, S.L., Huang, Y.S., and Chiang, A.N. (2006) L-Glutamate activates RhoA GTPase leading to suppression of astrocyte stellation. Eur. J. Neurosci. 23: 1977-1987.
Lin, K.Y., Wang, H.H., Lai, S.T., Pan, J.P., and Chiang, A.N. (2005) β2-Glycoprotein I protects J774A.1 macrophages and human coronary artery smooth muscle cells against apoptosis. J. Cell. Biochem. 94: 485-496.
Chen, C.J., Liao, S.L., Huang, Y.S., and Chiang, A.N. (2005) RhoA inactivation is crucial to manganese-induced astrocyte stellation. Biochem. Biophys. Res. Commun. 326: 873-879.
Wang, H.H., and Chiang, A.N. (2004) Cloning and characterization of the human β2-glycoprotein I (β2-GPI) gene promoter: roles of the atypical TATA box and HNF-1α in regulating β2-GPI promoter activity. Biochem. J. 380: 455-463.
Wang, H.H., Hung, T.M., Wei, J., and Chiang, A.N. (2004) Fish oil increases antioxidant enzyme activities in macrophages and reduces atherosclerotic lesions in apoE-knockout mice. Cardiovas. Res. 61: 169-176.
Chiang, A.N., Kou, T.C., and Wang, H.H. (2003) Influence of polyunsaturated fatty acids on the expression of PPARr in vascular cells and their effect on gene transcription. Atherosclerosis 4: 216.
Lin, K.Y., Chen, Y. L., Shih, C.C., Pan, J.P., Chen, W.E., and Chiang, A.N.（2002）The contribution of HDL-apolipoproteins to the inhibtion of low density lipoprotein oxidation and lipid accumulation in macrophages. J. Cell. Biochem. 86: 258-267.
Pan, J.P., Lai, S.T., Chiang, S.C., Chou, S.C., and Chiang, A.N.（2002）The risk of coronary artery disease in population of Taiwan is associated with cys-ser 311 polymorphism of human paraoxonase (PON)-2 gene. Chin. Med. J. 65: 415-421.
Lin, K.Y., Pan, J. P., Yang, D. L., Huang, K. T., Chang, M. S., Ding, P. Y. A., and Chiang, A. N. (2001) Evidence for inhibition of low density lipoprotein oxidation and lipid accumulation by apolipoprotein H (β2-glycoprotein I). Life Sci. 69: 707-719.
Lin, K.Y., Chen, Y. L., Chan, W. E., and Chiang, A.N. (2000) The role of HDL-apolipoproteins in low-density lipoprotein oxidation and cholesterol transport. J. Submicrosc. Cytol. Pathol. 32: 370. (SCI paper)
Chiang, A.N., and Lin, K.Y. (2000) The effects of β2-glycoprotein I on LDL oxidation and lipid accumulation in macrophages. J. Submicrosc. Cytol. Pathol. 32: 417. (SCI paper)