The functions of various hormones are mediated through their corresponding receptors, which serve as communicators between extracellular and intracellular signals. Among the present pharmaceutical products, more than 90% of marketed drugs are hormone derivatives or molecules designed to target the mechanisms conducted by hormone-receptor interactions. Therefore, in our lab we aim to discover the novel hormones and characterize the molecular actions of hormone receptors. Several strategies and research goals were briefly described below.

Project 1. Functional genomic approaches for novel hormone discovery.

Using computational tools to analyze the massive protein databases in species, homologies among polypeptide hormones can be sorted and placed into families. We have identified several novel endocrine genes based on evolutionary conservation. Following the gene identification, the recombinant hormones will be generated and the potential functions will be studied.

Project 2. Novel strategies for signal dissection and high-throughput screening of G protein-coupled receptors (GPCRs).

Sixteen G-alpha genes have been identified in mammals. However, current GPCR assays are limited in the evaluation of selective G-alpha activation, thus not allowing a comprehensive pathway screening. To facilitate orphan GPCR studies, we therefore propose a novel assay using G-alpha chimeras for GPCR signaling studies.

Project 3. Ovarian physiology.

Mammalian ovary is the organ that contains the largest numbers of endocrine as well as paracrine factors. The communication between these signaling molecules and receptors expressed in the oocyte, granulosa cells and theca/interstitial cells governs the follicular growth and ovarian development. Therefore, we are interested in exploring the potential function and physiological regulation of the novel hormones found in the ovary.