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姓名：蔡東湖
職稱：教授
電話：(02)2826-7115
傳真：(02)2822-5044
電子信箱：thtsai@ym.edu.tw
學經歷：
台北醫學院
藥學士
國立陽明大學
藥理學碩士
國立陽明大學
藥理學博士
英國劍橋大學
博士後研究
國立陽明大學
傳統醫藥學研究所副教授、教授
國立中國醫藥研究所 中藥及天然藥物研究組研究員兼組長
考選部 高等考試典試委員(2007)
香港大學 研究計畫海外審審查委員(2004~)
財團法人高等教育評鑑中心 高等教育評鑑委員2007
行政院國科會 專題研究計畫初審及複審委員
台北市立聯合醫院 人體試驗委員會委員
台北市立聯合醫院 實驗動物委員會委員
台北市立聯合醫院 實驗室生物安全委員會委員
台北市立聯合醫院 醫研中心委員會委員
台北市立聯合醫院 圖書委員會委員
台北市政府衛生局 儀器委員會委員
中華天然藥物學會 理事
科學期刊編審工作：
Editorial Board, Journal of
Chinese Medicine 2001~
Editorial Board, Taiwanese Pharmaceutical Journal 2001~
Editorial Board, Taipei Hospital Journal 2006~
Editor-in-Chief, John Wiley & Sons, Inc. Chief editor for a book of Application of Microdialysis in Pharmaceutical Science 2007
Editorial Board, Journal of Pharmaceutical and Biomedical Analysis, 2009~2011
國內外學術期刊與研究審查委員：
1. Analytica Chimica Acta
2. International Journal of Pharmaceutics
3. Journal of Agriculture Food and Chemistry
4. Journal of Biochemical and Biophysical Methods
5. Journal of Chromatography A
6. Journal of Chromatography B
7. Journal of Pharmacy and Pharmacology
8. Journal of Pharmaceutical and Biomedical Analysis
9. Journal of Pharmaceutical Sciences
10. Journal of Separation Science
11. Life Sciences
12. Methods and Findings in Experimental and Clinical Pharmacology
13. Molecular Nutrition and Food Research
14. Molecules
15. Pharmaceutical Research
16. Talanta
學術獎項：
Top referee 2007, Journal of Pharmaceutical and Biomedical Analysis
研究方向：
本研究室的研究主題是要探討一般藥物或天然藥物成分之藥物動力學及其藥效學。為了研發無體液流失的活體取樣方法，我們利用新的微透析取樣技術，配合微量偵測液相層析系統，進行藥物動力學研究。研究的範圍包括：
（１）微透析活體取樣技術的特性是，無蛋白質結合藥物的活體取樣，利用此特殊的取樣技術我們可以探討藥物在體內吸收、分佈、代謝、排泄等藥物動力學的研究。進一步為了避免膽汁的流失，我們實驗室自行研發設計流通型膽汁微透析活體取樣技術，進行藥物之膽汁排泄研究，以及探討藥物進行腸肝循環之研究。
（２）為了探討藥物穿透血腦屏障的機轉，我們將實驗室自行設計同心管型微透析探針分別植入同一隻活體動物的腦組織和血管中進行藥物在血和腦的檢品同時取樣，再分別計算中樞神經系統和周邊循環系統之藥物動力學結果。相同的技術也可以應用在神經傳遞物質釋放之藥效學研究。
（３）進一步，微透析活體取樣技術也用在活體捕捉氫氧自由基的研究，我們利用此技術來探討藥物消除氫氧自由基的研究。
To investigate the pharmacokinetics of free form drug molecular, we have been utilizing microdialysis technique for sampling to avoid the body fluid loss of the subject due to continuous sampling. We have also developed various high-performance liquid chromatography with different detection methods to perform pharmacokinetic studies (Tsai, T.H., 2003, Assaying protein unbound drugs using microdialysis techniques. Journal of Chromatography B. 797:161-173).Recently, the applicant (Dr. Tung-Hu Tsai) was invited by Elsevier to contribute a chapter related to the application of microdialysis with mass spectrometric detection in the Encyclopedia of Mass Spectrometry in 2006.
Investigation of drug metabolism using mass spectrometry
The principle of microdialysis is to exclude macromolecules by the dialysis membrane, and it can be used to sample protein unbound drug molecules. We have been applying this technique to elucidate the in vivo absorption, distribution, metabolism and elimination for drugs of interest. With the help of mass spectrometry, the mechanism of drug biotransformation can be explored. We have been introducing the modern and novel technique to improve the level of Chinese medicine research (Tsai, P.L., Tsai, T.H., 2004, Hepatobiliary excretion of berberine.
Drug Metabolism and Disposition. 32:405-412)
Hepato-biliary circulation of drugs using microdialysis
We have designed the special microdialysis probe for bile sampling purpose to avoid bile loss and to investigate the biliary excretion and hepato-biliary circulation of drugs. Results related to hepato-biliary circulation of drugs using microdialysis have been published and cited by other investigators in 2000-2006 (Tsai, T.H., Shum, A.Y.C., Chen, C.F., 2000, Enterohepatic circulation of chloramphenicol and its glucuronide in the rat by microdialysis using a hepato-duodenal shunt. Life Sciences. 66:363-370).
Drug transportation in the blood-brain barrier using microdialysis
We implanted two concentric microdialysis probes into brain tissue and blood vessel on the same experimental animal for simultaneous sampling, and the pharmacokinetic profiles of central nervous and peripheral system can estimated (Tsai, T.H., 2001, Pharmacokinetics of pefloxacin and its interaction with cyclosporin A, a P-glycoprotein modulator, in rat blood, brain and bile, using simultaneous microdialysis. British Journal of Pharmacology. 132: 1310-1316). |
