王湘翠 助理教授

助理教授

E-mailhtwang01@ym.edu.tw

電話:02-28267097

地址:陽明大學藥理學研究所研究大樓四樓461

 

學歷

博士

美國紐約大學,醫學院環境醫學研究所

毒理暨分子致癌機轉組

2008-2012

碩士

台灣大學,醫學院生物暨分子生物研究所

2004-2006

學士

台灣大學,藥學系

2001-2004

 

現職和經歷

助理教授

陽明大學,藥理學研究所

2014迄今

博士後研究員

美國紐約大學,Langone醫學中心

2012-2014

研究助理

美國紐約大學,醫學院環境醫學所

2007-2008

教學助教

台灣大學,醫學院生物暨分子生物研究所

2006-2007

 

榮譽獎項

        Post-Doctoral Fellowship Award,美國癌症協會,2013/07

        ACS Travel Grant紐約大學Langone醫學中心泌尿科2013/05

        GSAS Dean's Student Travel Grant美國紐約大學2012/04

        藥師執照,台灣衛生署,2004/08

        學術表現傑出獎,台灣大學,藥學系,2004/06

        學士研究獎,台灣財團法人景康藥學基金會,2004/06

        傑出人才獎,台灣大學醫學院,2004/03

        台灣藥學會3rd IPSF-Asia Pacific Pharmaceutical Symposium, Taiwan        2003/07.

 

主要研究方向

1.     對於環境中肺癌致癌因子以及致癌機制的研究

2.     發展早期檢測肺癌之biomarkers以及對於肺癌預防方法的研究。

目前研究的主題是以acrolein對於肺癌致癌機制的探討,acrolein是一個極度不穩定且有活性的醛類,會與細胞中的DNA以及DNA修復蛋白質作用進而影響細胞正常功能。Acroelin 不僅在環境中可以發現,例如:車輛排放的廢氣、香菸以及炒菜油煙中;它也存在於人體體內,acroleinlipid peroxidation的副產物,研究顯示當細胞產生oxidative stress時會產生高含量的acrolein。目前對於acrolein的致癌機轉仍不清楚,本實驗之研究主題將以了解acrolein如何造成肺癌的機轉加以探討,進一步希望可以發展出肺癌早期診斷之biomarker以及預防肺癌的方法。

On-going projects:

i.           The role of acrolein in mitochondria DNA damage, repair and mutation.

ii.         Differential effects of acrolein-induced mitochondrial dysfunction on normal and cancerous cells: involvement of mitochondrial DNA damages and mitophagy.

iii.       Acrolein induces ribosomal stress in human cells.

 

國際期刊發表

1.     Lee HW*, Wang HT*, Weng MW, Chin C, Huang W, Lepor H, Wu XR, Rom WN, Chen LC, Tang MS. Cigarette side-stream smoke lung and bladder carcinogenesis: inducing mutagenic acrolein-DNA adducts, inhibiting DNA repair and enhancing anchorage-independent-growth cell transformation. Oncotarget. 2015 Sep 28. [Epub ahead of print] (* equal contribution)

2.     Lee HW*, Wang HT*, Weng MW, Hu Y, Chen WS, Chou D, Liu Y, Donin N, Huang WC, Lepor H, Wu XR, Wang H, Beland FA, Tang MS. Acrolein- and 4-Aminobiphenyl-DNA adducts in human bladder mucosa and tumor tissue and their mutagenicity in human urothelial cells. Oncotarget. 2014 Jun 15;5(11):3526-40. (* equal contribution)

3.     Wang HT, Weng MW, Chen WC, Yobin M, Tang MS. Effect of CpG Methylation at Different Sequence Context on Acrolein-DNA Binding and Mutagenesis. Carcinogenesis; 34:220-7(2013).

4.     Pan J, Awoyemi B, Xuan Z, Vohra P, Wang HT, Dyba M, Greenspan E, Fu Y, Creswell K, Zhang L, Berry D, Tang MS, Chung FL. Detection of Acrolein-Derived Cyclic DNA Adducts in Human Cells by Monoclonal Antibodies. Chem Res Toxicol; 25:2788-95(2012).

5.     Wang HT, Hu Y, Tong D, Huang J, Gu L, Wu XR, Chung FL, Li GM, Tang MS. Effect of carcinogenic acrolein on DNA repair and mutagenic susceptibility. J Biol Chem; 287(15):12379-12386. (2012)

6.     Tang MS, Wang HT, Hu Y, Chen WS, Akao M, Feng Z, Hu W. Acrolein induced DNA damage, mutagenicity and effect on DNA repair. Mole Nut and Food Res ; 55(9):1291-300 (2011).

7.     Wang HT, Choi B, Tang MS. Melanocytes are deficient in repair of oxidative DNA damage and UV-induced photoproducts. Proc Natl Acad Sci U S A; 107(27):12180-5. (2010).

8.     Wang HT, Zhang S, Hu Y, Tang MS. Mutagenicity and sequence specificity of acrolein-DNA adducts. Chem Res Toxicol; 22(3):511-7. (2009)

9.     Wang HT, Tang MS. Acrolein-DNA Adducts Are Mutagenic. Chem. Res. Toxicol; 22 (5):753–754. (2009)

 

邀請演講

1.       Effect of acrolein on mitochondrial DNA damage response. Joint Conference of Cell signaling in China, Hang Zhou, China, 10/2015

2.       Aflatoxin B1 hepatocarcinogenesis via lipid peroxidation inducing cyclic propano-DNA adduct at p53 codon 249, inhibiting DNA repair and enhancing mutation susceptibility. Society of Chinese Bioscientists in America, Taiwan, 06/2015

3.       The effect of Acrolein in DNA Damage, DNA Repair and Lung Carcinogenesis. Toxicology society of Taiwan, Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taiwan, 03/2014

 

研討會議Poster發表

1.     Chen TY, Wang HT *, Acrolein induces ribosomal stress in human cells. Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taiwan, 2016

2.     CW Weng, JH Lin, HT Wang *, Effect of acrolein on mitochondrial DNA damage response, Society for Mitochondrial Research and Medicine, National Yang Ming University, Taiwan, 2015

3.     Wang HT, MW Weng, HW Lee , Huang WC, Liu Y, Lepor H, Wu XR and Tang MS. The Role of p63 and p53 Mutant in the Regulation of DNA Repair, Mutator and Invasive Phenotype of Bladder Cancer Cells. Society of Chinese Bioscientists in America, Academia Sinica, Taiwan, 2015

4.     Wang HT, Lee HW, Weng MW, Kuo J, Huang W, Lepor H, Donin N, Wu XR, Tang MS. Invasive bladder cancer cells are deficient in DNA repair and have mutator phenotypes that are due to suppression of p63 gene expression. American Urology Association, 2013

5.     Lee HW, Wang HT, Weng MW, Liu Y, Donin N, Huang W, Wu XR, Lepor H, Tang MS. Quantitation of DNA adduct induced by bladder carcinogens - aminobiphenyl and acrolein -in normal human urothelial mucosa and bladder tumor tissue. American Urology Association, 2013

6.     Wang HT, Hu Y, Tong D, Huang J, Gu L, Wu XR, Chung FL, Li GM, Tang MS. Effect of carcinogenic acrolein on DNA repair and mutagenic susceptibility. American Association for Cancer Research Annual Meeting, 2012

7.     Wang HT, Choi B, Tang MS. Melanocytes are deficient in repair of oxidative DNA damage and UV-induced photoproducts. American Association for Cancer Research Annual Meeting, 2010

8.     Wang HT, Kuo LH, Huang W, Wu XR, Tang MS. Invasive and non-invasive bladder cancer cells have different DNA excision repair capacity and mutator phenotypes."Recent Advances in Urological Research Symposium" sponsored by NYU medical center, 2010