|Ph.D.||Univ. of Manitoba Canada|
|Vice Dean, Academia Sinica|
|Research Fellow, Academia Sinica|
|Associate Research Fellow, Academia Sinica|
¡@¡@As a member of the Division of Infectious Diseases, our laboratory focuses
our effort on two topics: (1) study of the mechanism of genesis of drug resistance
and gene amplification in protozoan Leishmania parasites and the mechanism(s)
of infection of Leishmania parasite to host mononuclear phagocytes and (2) development
of subunit and DNA vaccines against flavivirus.
In drug resistance: (i) a genome wide search of the genes activated during resistance induction in order to understand the mechanisms of genesis behind drug resistance and gene amplification. Subtractive hybridization and proteomic approaches are used for a genome wide comparison between the wildtype and the drug-resistant Leishmania; (ii) an intercellular adhesive molecule (ICAM) cloned and expressed is used to investigate the mechanism involved in the interaction between the parasite and its host macrophage.
In the flaviral vaccine study, we focus on the development of subunit and DNA vaccines against Japanese encephalitis virus (JEV) which is an important pathogen in Taiwan as well as in Eastern and Southeastern Asia. A C-terminal fragment of JEV envelope (E) protein was found to induce protective immunity against JEV. We are now mapping the smallest and most immunodominant fragment of this antigen for inducing protective immunity against JEV using DNA priming-protein boosting strategies.
¡@Selected Recent Publication :Lye, L.F., Hsu J.Y., Singh, A.K., Su, K.E. and
Lee, S. T.
Characterization by pulse field electrophoresis of a new region of DNA amplification containing the M2 subunit of ribonucleotide reductase in the hydroxyurea-resistant Leishmania. Parasitol. Res. 85:188-193, 1999.
Singh, A.K. and Lee, S.T.
Status of respiration and ATP content in arsenite resistant Leishmania mexicana amazonensis. Microbiol. Pathogen. 26(3): 171-174, 1999.
Lye, L.F., Chiang, S.C., Hsu, J.Y. and Lee, S.T.
Expression and cellular localization of ribonucleotide reductase small subunit M2 protein in hydroxyurea-resistant Leishmania mexicana amazonensis Mol. Biochem. Parasitol. 102: 263-271, 1999.
Chiang, S.C., Ali, V., Haung, A.L., Chu K.U., and Lee, S.T.
Molecular, cellular and functional characterizations of a novel ICAM-like molecule of the Ig-superfamily from L. m. amazonensis. Mol. Biochem. Parasitol. 112: 263-275, 2001.
Chia, S.C., Leung, P.S.C., Liao, C.P., Huang, J.H. and Lee, S.T.
Fragment of Japanese encephalitis virus envelope protein produced in Escherichia coli protects mice from virus challenge. Microbial Pathogenesis 31(1): 9-19, 2001.
Chang, Y.H., Lee, S.T. and Lin, W.W.
Effects of cannabionoids on LPS-stimulated inflammatory mediator release from macrophages: involvement of Eicosanoids. J. Cell. Biochem. 81: 715-723, 2001.
Chiang, S.C., Chang, S.C. and Lee, S.T.
ICAM-L gene is conserved only in Leishmania species in the family of kinetoplastida. Mol. Biochem. Parasitol. 124: 47-50, 2002.
Hsu, M.J., Lee, S.S., Lee, S.T. and Lin, W.W.
Signaling mechanisms of enhanced neutrophil phagocytosis and chemotaxis by the polysaccharide purified from Ganoderma lucidum. Br. J. Pharmacol. 139(2): 289-98, 2003.
Chen, C.W., Lee, S.T., Wu, W.T., Fu W.M., Ho, F.M. and Lin, W.W.
Signal transduction for inhibition of inducible nitric oxide synthase and cyclooxygenase-2 induction by capsaicin and related analogs in macrophages. Br. J. Pharmacol. 140: 1077-1087, 2003.
Wu, H.W., Chen, C.T., Lin, Y.L. and Lee, S.T.
Subfragments of the envelope gene are highly protective against the Japanese encephalitis virus lethal infection in DNA priming/protein boosting immunization strategies. Vaccine, 22: 793-800, 2004.