Dr. Yu-Jiun Chan, Adjunct Associate Professor

Education：

Experience：

Membership：

台灣內科醫學會會員

中華民國感染症醫學會會員

台灣愛滋病學會（原中華民國愛滋病防治協會）秘書長

Expertise：

內科學、分子生物學、分子病毒學、感染症

論文指導：

國立清華大學生命科學研究所碩士生王書品論文口試委員（ 91 年 5 月），

　題目：台灣北區多重抗藥菌株研究及其檢驗用基因晶片開發。

國立清華大學生命科學研究所碩士生郝旭昶論文口試委員（ 91 年 7 月），

　題目：基因晶片系統基因純化及應用於 CMV 病毒感染之研究。

國立陽明大學公共衛生研究所碩士生賴淑芬論文口試委員（ 92 年 7 月），

　題目：台灣男同志三溫暖顧客感染 HIV 之分子流行病學研究。

Publish：

【A】博士畢業論文

Dissertation: A model for CMV latency and reactivation: role of cellular factors and signal transduction in transcriptional regulation of the CMV immediate-early gene promoter/enhancer regions. By Yu-Jiun Chan, the Johns Hopkins University, 1996.

【B】學術論文

1. Wu,TC, W A Lee, M C Pizzorno, W C Au, Y J Chan , R H Hruban, G M Hutchins, G S Hayward, 1992, Localization of the human cytomegalovirus 2.7-kb major early beta-gene transcripts by RNA in situ hybridization in permissive and nonpermissive infections: Am.J.Pathol., v. 141, p. 1247-1254.
2. Chang,YN, K T Jeang, C J Chiou, Y J Chan , M Pizzorno, G S Hayward, 1993, Identification of a large bent DNA domain and binding sites for serum response factor adjacent to the NFI repeat cluster and enhancer region in the major IE94 promoter from simian cytomegalovirus: J.Virol., v. 67, p. 516-529.
3. Chan,YJ , C J Chiou, Q Huang, G S Hayward, 1996, Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus major immediate-early promoters in monocyte and T-lymphocyte cell types: J.Virol., v. 70, p. 8590-8605.
4. Chan,YJ , W P Tseng, G S Hayward, 1996, Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus: J.Virol., v. 70, p. 5312-5328.
5. Wu,TC, D A Fuentes-Bernardo, Y J Chan , W C Au, C J Chiou, W M Fox, III, R H Hruban, G S Hayward, R J Kurman, 1997, Detection of the Human Cytomegalovirus 2.0-kb Immediate Early Gene 1 Transcripts in Permissive and Nonpermissive Infections by RNA in situ Hybridization: J.Biomed.Sci., v. 4, p. 19-27.
6. Lee,CH, G C Lee, Y J Chan , C J Chiou, J H Ahn, G S Hayward, 1999, Factors affecting human cytomegalovirus gene expression in human monocyte cell lines: Mol.Cells, v. 9, p. 37-44.
7. Huang,YH, J C Wu, T Y Chiang, Y J Chan , T I Huo, Y S Huang, S J Hwang, F Y Chang, S D Lee, 2000, Detection and viral nucleotide sequence analysis of transfusion- transmitted virus infection in acute fulminant and non-fulminant hepatitis: J.Viral Hepat., v. 7, p. 56-63.
8. Chiou,SH, J H Liu, W W Wong, Y J Chan , Y C Chang, J J Wang, C Y Liu, W T Liu, S C Chen, W M Hsu, 2000, Detection of human cytomegalovirus retinitis and monitoring of ganciclovir treatment using conjunctival swab with polymerase chain reaction in AIDS patients: Int.J.STD AIDS, v. 11, p. 85-91.
9. Chiou,SH, C Y Liu, W M Hsu, Y J Chan , C K Chou, Y M Chung, J H Liu, W T Liu, S C Chen, W W Wong, 2000, Ophthalmic findings in patients with acquired immunodeficiency syndrome: J.Microbiol.Immunol.Infect., v. 33, p. 45-48.
10. Chan,YJ , Y H Hsu, M C Chen, W W Wong, J C Wu, W C Yang, C Y Liu, 2000, TT virus infection among hemodialysis patients at a medical center in Taiwan: J.Microbiol.Immunol.Infect., v. 33, p. 14-18.
11. Fung,CP, Y M Lee, B I Kuo, S P Yang, Y J Chan , C Y Liu, L T Ho, W T Liu, 2000, Using buffy coat for reverse transcriptase-polymerase chain reaction in the diagnosis of dengue virus infection: preliminary study: J.Microbiol.Immunol.Infect., v. 33, p. 217-222.
12. Chan YJ, Y W Ma, Y C Liu, C Y Liu, W T Liu, Identification of two major human CMV gB subtypes from medical centers on Taiwan. (manuscript in preparation)
13. Chan YJ, Y M Lee, C Y Liu, C H Lee, Y M Chen, Establishment of a Western Blot Confirmatory Assay for SARS and Comparison of Different Commercial Kits (manuscript in preparation)

【C】研究計畫

1. 新種TT病毒感染及致病機轉之研究
   　　編號： VGH415-12
   　　時間： 88年1月1日~88年6月30日
2. 巨細胞病毒(CMV)活體外復發機轉模型研究
   　　編號： VGH89-39
   　　時間： 88年7月1日~89年12月31日
3. 新種TT病毒(TTV)感染及致病機轉之研究-展延計畫
   　　編號： VGH89-398-7
   　　時間： 88年7月1日~89年12月31日
4. 鏈黴菌研究-鏈黴菌類核體之結構
   　　編號： NSC89-2316-B-075-001-M44
   　　時間： 86年8月1日~89年7月31日
5. Sensitivity and specificity comparison of viral culture, nested PCR, SARS PCR-ELISA, and real-time PCR on the diagnosis of the SARS-coronavirus
   　　編號： VGH393-9
   　　時間： 92年8月1日~92年12月31日
6. Prospective study the diagnostic modalities of rapid differentiating SARS and non-SARS atypical pneumonia
   　　編號： CSTVGH92-03
   　　時間： 92年11月1日~93年10月31日

【 D 】國際會議發表研究成果

1. Chan YJ , C J Chiou, G S Hayward. Basal enhancer and repressor domains and phorbol ester (PKC) inducible elements in HCMV and SCMV immediate-early promoters. The 18 th International Herpes virus Workshop, Pittsburgh, U. S. A. July 25-30, 1993, abstract A98 (oral)
2. Chan YJ , Y H Hsu, W W Wong, W C Yang, C Y Liu. A novel TT virus (TTV) infection in hemodialysis patients in a medical center. The 1 st International Congress of the Asia Pacific Society of Infection Control (APSIC), Hong Kong, August 9-11, 1999, abstract p1.13 (poster)
3. Lin YR, C C Chen, Y J Chan . Purification of telomere-binding proteins of Streptomyces lividans. The 11 th International Symposium on the Biology of Actinomycetes, Sissi-Heraklion, Crete, Greece, October 24-28, 1999, abstract (poster)
4. Chan YJ , J H Lin, W W Wong, C P Fung, C Y Liu, 2000. Two novel TEM-like β -like genes cause multiple-drug resistant infections in a medical center in Taiwan. The 40 th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), Toronto, Canada, September 17-20, 2000, abstract 1997 (poster)