Wailap Victor Ng (吳韋訥) Laboratory

Research Interests

Systems biology – Apply DNA microarray, mass spectrometry, 2-hybrid expression system, and bioinformatics technologies to (i) characterize the biomodules essential for cell survival upon environmental perturbations in Halobacterium sp. NRC-1 and Haloarcula marismortui and (ii) identify the new functions of more than 50% of all the predicted genes in these microorganisms.

Biomarker discovery–Identify cancer diagnostic markers and therapeutic targets using bioinformatics and mass spectrometry tools.

Technology development – EST database mining, mass spectrometry, and mass spectrometry data analysis system.

Contact Information

Address Institute of Biotechnology in Medicine, National Yang Ming University, 155 Li Nong St., Section 2, Pei Tou, Taipei, Taiwan, R.O.C.

Phone 02-2826-7321 (office)

Fax 02-2826-4092 (fax)

Email wvngym.edu.tw

Current Position

2003–Present Associate Professor, National Yang Ming University, Institute of Biotechnology in Medicine, Institute of Bioinformatics, Department of Biotechnology and Laboratory Science in Medicine, Taiwan

Education

1985 National Yang Ming Medical College, Taiwan. (B.S. Medical Technology)

1993 University of Massachusetts, Amherst, MA, USA. (Ph.D. Microbiology)

Professional Experience

1985-1987 Teaching Assistant, Department of Medical Technology, National Yang Ming Medical College, Taipei

1993-1997 Senior Fellow, Department of Molecular Biotechnology, University of Washington, Seattle, WA, USA (Supervisor: Leroy Hood)

1997-2000 Research Associate, Department of Molecular Biotechnology, University of Washington, Seattle, USA (Supervisor: Leroy Hood)

2000-2003 Senior Research Scientist, Institute for Systems Biology, Seattle (Supervisor: Leroy Hood)

2003-Present Associate Professor, National Yang Ming University, Taiwan

Research Funding

8/2003-7/2006 Systems Biology of Halobacterium Species NRC-1: Molecular Adaptation to Temperature Changes (NSC92-2314-B-010-055; NT$2,420,600)

Publications

Cancer

Schummer, M., Ng, W.V., Bumgarner, R.E., Nelson, P.S., Schummer, B., Bednarski, D.W., Hassell, L., Baldwin, R.L., Karlan, B. Y., and Hood, L. 1999. Comparative hybridization of an array of 21,500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas. Gene 238:375-85.

Huang, G.M., Ng, W.-L., Farkas, J., He, L., Liang, H.A., Gordon, D., Yu, J., and Hood, L. 1999. Prostate cancer expression profiling by cDNA sequencing analysis. Genomics 59:178-86.

Wang, K, Gan, L., Jeffery, E., Gayle, M., Gown, A.M., Skelly, M., Nelson, P.S., Ng, W.V., Schummer, M., Hood, L., and Mulligan, J. 1999. Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray. Gene 229:101-8.

Nelson, P.S., Ng, W.-L., Schummer, M., True, L.D., Liu, A.L., Bumgarner, R., Ferguson, C., Dimak, A., and Hood, L. An expressed-sequence-tag database of the human prostate: Sequence analysis of 1,168 cDNA clones. Genomics 47: 12-25, 1998.

Genomics

Baliga, N.B., Bonneau, R., Facciotti, M.T., Pan, M., Glusman, G., Deutsch, E.W., Shannon, P., Chiu, Y., Weng, R.S., Gan, R.R., Hung, P., Date, S., Marcotte, E., Hood, L., Ng, W.V. Genome of Haloarcula marismortui – a halophilic archaeon from the Dead Sea. Genome Research 14:2221-34.

Goo, Y.A., Roach, J., Glusman, G., Baliga, N.S., Deutsch, K., Pan, M., Kennedy, S., DasSarma, S, Ng W.V., Hood L. 2004. Low-pass sequencing for microbial comparative genomics. BMC Genomics. 5:3-21.

Ng, W.V. et al. 2000. Genome sequence of Halobacterium sp. NRC-1. Proc Natl Acad Sci 97:12176-81.

Ng, W.-L. et al. 1998. Snapshot of a large dynamic replicon in a halophilic archaeon: megaplasmid or minichromosome? Genome Res. 8:1131-41.

Proteomics

Goo, Y.A., Yi, E.C., Baliga, N.S., Tao, W.A., Pan, M., Aebersold, R., Goodlett, D.R., Hood, L., Ng, W.V. 2003. Proteomic Analysis of an Extreme Halophilic Archaeon, Halobacterium sp. NRC-1. Mol Cell Proteomics. 2:506-24.

Microbiology

Baliga, N.S., Pan, M., Goo, Y.A., Yi, E.C., Goodlett, D.R., Dimitrov, K., Shannon, P., Aebersold, R., Ng, W.V., Hood, L. 2002. Coordinate regulation of energy transduction modules in Halobacterium sp. analyzed by a global systems approach. Proc Natl Acad Sci U S A 99:14913-8

Kennedy, S.P., Ng, W.V., Salzberg, SL., Hood, L., DasSarma, S. 2001. Understanding the adaptation of Halobacterium species NRC-1 to its extreme environment through computational analysis of its genome sequence. Genome Res. 11:1641-50.

Baliga, N.S., Kennedy, S.P., Ng, W.V., Hood, L., DasSarma, S. 2001. Genomic and genetic dissection of an archaeal regulon. Proc Natl Acad Sci U S A 98:2521-5.

Peck, R.F., Echavarri-Erasun, C., Johnson, E.A., Ng, W.V., Kennedy, S.P., Hood, L., DasSarma S., Krebs, M.P. 2001. brp and blh are required for synthesis of the retinal cofactor of bacteriorhodopsin in Halobacterium salinarum. J. Biol. Chem 276:5739-44.

DasSarma, S., Kennedy, S.P., Berquist, B., Ng, W.V., Baliga, N.S., Spudich, J.L., Krebs, M.P., Eisen, J.A., Johnson, C.H., Hood, L. 2001. Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon. Photosyn. Res. 70:3-17.

Baliga, N.S., Goo, Y.A., Ng, W.V., Hood, L., Daniels, C.J., and DasSarma, S. 2000. Is gene expression in Halobacterium NRC-1 regulated by multiple TBP and TFB transcription factors? Mol. Microbiol. 36:1184-5

Ng, W.-L., C.-F. Yang, J.T. Halladay, P. Arora, and S. DasSarma. 1995. Isolation of genomic and plasmid DNA from Halobacterium halobium. In S. DasSarma and E.M. Fleishmann (ed.), “Archaea: A laboratory manual - Halophiles”, Cold spring Harbor Laboratory press, p155-160.

Ng, W.-L., Arora, P., and DasSarma, S. 1994. Large deletion in class III gas vesicle-deficient mutants of Halobacterium halobium. System. Appl. Microbiol. 16: 560-568.

Bobovnikova, Y., Ng, W.-L., DasSarma, S., and Hackett, N.R. 1994. Restriction mapping the genome of Halobacterium halobium strain NRC-1. System. Appl. Microbiol. 16: 597-604.

Ng, W.-L. and DasSarma, S. 1993. Minimal replication origin of the 200 kilobase Halobacterium plasmid pNRC100. J. Bacteriol. 175: 4584-4596.

Halladay, J.T, Ng, W.-L., and DasSarma, S. 1992. Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float. Gene 119: 131-136.

Ng, W.-L., Kothakota, S., and DasSarma, S. 1991. Structure of the gas vesicle plasmid in Halobacterium halobium: inversion isomers, inverted repeats, and insertion sequences. J. Bacteriol. 173:1958 1964.

Ng, W.-L. and DasSarma ,S. 1991. Physical and genetic mapping of the unstable gas vesicle plasmid in Halobacterium halobium NRC-1. In F. Rodriguez-Valera (ed.), “General and applied aspects of halophilic microorganisms”, Plenum Press, New York, p.305-311.

Ng, W.-L., Halladay, J.T., and DasSarma, S. 1991. Isolation of large Halobacterium halobium plasmids, p. 3.5.1-3.5.3. In E.M. Fleischmann, A.R. Place, F.T. Robb, and H.J. Schreier (ed.), Protocols for archaebacterial research. University of Maryland, Baltimore.

J.G. Jones, Hackett, N.R.,. Halladay, J.T, Scothorn, D.J., Yang, C.-F., Ng, W.-L., and DasSarma, S. 1989. Analysis of insertion mutants reveals two new genes in the pNRC100 gas vesicle cluster of Halobacterium halobium. Nucleic Acids Res. 17:7785-7793.

Technologies

Nelson, P.S., Hawkins, V., Schummer, M., Bumgarner, R, Ng, W.-L., Ideker, T., Ferguson, C., and Hood, L. 1999 Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays. Genet Anal 15:209-15.

M. Schummer, Ng, W.-L., Nelson, P. S., Bumgarner, R.E, and Hood, L. An inexpensive and-held device for the construction of high density nucleic acid arrays. Biotechniques 23: 1087-1092, 1997.

Ng, W.-L., Schummer, M., Cirisano, F.D., Baldwin, R.E., Karlan, B.Y., and Hood, L. 1996. High-throughput plasmid mini preparation facilitated by micro-mixing. Nucleic Acids Res. 24:5045-5047.