論文名稱:

神經生長因子誘導以酵素分離之牙乳突分化成類神經細胞

 

Nerve Growth Factor induced Neural-like cells differentiation in Dispase Separated Dental papilla

研究生:

郭玟君  Wen-Chun Kuo

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

楊世芳  Shih-Fang Yang

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

            學號:

870H03

          學年度:

88

          語文別:

中文

          出版年:

89

關鍵字:

神經生長因子  Nerve growth factor

 

牙乳突  Dental papilla

全文說明:

電子全文

        論文頁數:

52

摘要:

神經生長因子在神經系統的發育過程中被廣泛的研究,目前已知神經生長因子是由標的組織所衍生的一種生長因子,它可以促使神經元細胞或神經系統中其他細胞的存活及分化。但根據本實驗室先前利用免疫組織化學法研究結果發現,神經生長因子也能參與牙齒發育,其與牙齒形成的啟始期、形態發育期及細胞分化期皆有相當關係。在以神經生長因子之抗體阻斷神經生長因子的作用後,發現在發育的牙胚中之造牙本質細胞分化停止,得知神經生長因子對造牙本質細胞的分化有直接影響。然而,神經生長因子在牙胚中的表現究竟是誘導細胞發育的主要因子或僅是一個輔助因子。本研究將牙胚之上皮與間葉細胞分離,再加入神經生長因子,以觀察在沒有上皮組織的影響下神經生長因子單獨對牙乳突中間葉細胞的影響。為實驗對象。以蛋白質酵素Dispase,將胚胎第15171921S.D.品系之大白鼠的牙胚之上皮與間葉組織分離。再將牙乳突培養在含100 ng/ml神經生長因子或不含神經生長因子的培養液中七天。結果發現神經生長因子可促使多量的細胞自牙乳突組織中爬行出來,且其中有相當數目之形狀極似神經細胞,以PerpherinGlial fibrillary acidic proteinNeurofilament-160 kDa等三種染神經細胞的標誌來染該類神經細胞皆呈現正反應。本實驗結果在非神經性的組織中發現神經性細胞的生成,其意義為神經生長因子可使非神經性細胞分化成神經細胞,或神經生長因子可使某些特定具神經細胞潛能之細胞發育成神經細胞,還是神經細胞與造牙本質細胞為同一種細胞,則需進一步研究。

   
 

Nerve growth factor (NGF) has been widely studied in the development of the nerve system. Previous studies showed NGF may serve as an important factor for development of tooth, in initiation, morphogenesis and cytodffereniation. For observing the impact of NGF alone on odontoblast differentiation without dental epithelium , we used the day 15, day 17, day 19 and day 21 tooth germ from the embryo of S.D. rat for experimental models. The dental papilla and dental epithelium were separated by dispase treatment. Separated dental papilla was cultured in DMEM-serum medium with or without NGF for 7 days. The cells of dental papilla were be observed by inverted microcopy. Neural specific markers, peripherin, glial fibrillary acidic protein and neurofilament-M have been used to identify these cells by immunohistochemically. We found that some neural-like cells appeared after NGF treatment for 7 days and those cells responsed positively to those neural markers. The origin and the relationship to odontoblasts and neural crest cells will be characterized in the future.