論文名稱:

引導組織再生膜受致病菌污染或抗生素處理後對牙周韌帶細胞附著影響之研究

 

Attachment of Periodontal Ligament Cells Over Guided Tissue Regeneration Membranes Treated with Pathogens or Antibiotics

研究生:

林怡  Yi-Wen Lin

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

洪善鈴  Shan-Ling Hung

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

            學號:

870H01

          學年度:

88

          語文別:

中文

          出版年:

89

關鍵字:

引導組織再生膜  Guided Tissue Regeneration Membrane

 

致病菌  Pathogen

 

抗生素  Antibiotic

 

牙周韌帶細胞  Periodontal Ligament Cell

 

細胞附著  Cell Attachment

        論文頁數:

107

摘要:

牙周炎(periodontitis)會造成牙周組織的破壞,引導組織再生(Guided tissue regenerationGTR)術是治療上重要的一部份。本論文以活體外實驗模式來探討牙周韌帶細胞在Gore-TexResolut XTBioMend三種不同再生膜上的附著情形,並比較三種再生膜分別受兩種細菌,黏液鏈球菌(Streptococcus mutans)或放線共生放線桿菌(Actinobacillus actinomycetemcomitans)污染後,或分別以兩種抗生素tetracyclineamoxicillin處理後,對於牙周韌帶細胞附著的影響。首先以alkaline phosphatase (ALP)活性分析及von Kossa染色鑑別所培養的牙周韌帶細胞確實具有骨礦化特性。接著以MTT (3-4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium bromide)測試法分析再生膜上細胞附著情形,也以掃描式電子顯微鏡(scanning electronic microscopySEM)觀察附著細胞的形態及數目,結果發現Gore-Tex再生膜最不利於牙周韌帶細胞的附著。當三種再生膜分別受S. mutansA. actinomycetemcomitans污染後,再生膜上牙周韌帶細胞附著明顯受到抑制。以殺菌力生物分析法(antibiotic bioassay)分析含抗生素之再生膜其抗生素釋放力,結果顯示,三種再生膜利用滴上製備法可有效的將tetracyclineamoxicillin結合到再生膜上,並再釋放出來,含抗生素之再生膜並不會影響牙周韌帶細胞的附著,且含tetracyclineamoxicillin再生膜可改善S. mutansA. actinomycetemcomitans污染對牙周韌帶細胞附著所造成的影響。本論文之結果可提供未來牙科臨床再生膜及抗生素使用選擇上的參考。

   
 

Periodontitis will result in the destruction of periodontal tissues. Guided tissue regeneration (GTR) procedure is an important part in curing periodontal destruction. This thesis was to investigate periodontal ligament (PDL) cells attachment on Gore-Tex, Resolut XT and BioMend GTR membranes by an in vitro model, and compare the PDL cells attachment on three GTR membranes contaminated with two oral pathogens, Strepotococcus mutants, or  Actinobacillus actinomycetemcomitans and incorporated with two different antibiotics, tetracycline or amoxicillin. First of all, we confirmed the osteogenic characteristics of primary PDL cell cultured by applying alkaline phosphatase (ALP) activity assay and von Kossa staining. MTT (3-4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium bromide) test was used to analyze the number of cells attached onto GTR membranes. Scanning electronic microscopy (SEM) was also used to observe the morphology of cells and to quantitate the number of cells attached onto GTR membranes. The data indicated that Gore-Tex membrane was the worst substrate for PDL cells attachment. The number of cells attached onto GTR membranes was significantly decreased when GTR membranes were contaminated with S. mutants or A. actinomycetemcomitans. Antibiotic bioassay was used to analyze the amount of antibiotics released from three different membranes incorporated with two different antibiotics. The data indicated that antibiotics could be incorporated and released efficiently from antibiotics-loaded GTR membranes which were prepared by the drop method. Antibiotics-loaded GTR membrane did not affect the cells attachment. The effects of S. mutants or A. actinomycetemcomitans on cellular attachment were reduced on antibiotics-loaded GTR membranes. These findings may provide reference to select and apply GTR membranes and antibiotics for GTR therapy.

   

        發表著作

Hung S-L, Lin Y-W, Wang Y-H, Chen Y-T, Su C-Y, Ling L-J. Permeability of Streptococcus mutans and Actinobacillus actinomycetemcomitans through guided tissue regeneration membranes and their effects on attachment of periodontal ligament cells. Journal of Periodontology 2002; 73(8): 843-851.

Hung S-L, Lin Y-W, Chen Y-T, Ling L-J. Attachment of periodontal ligament cells onto various antibiotics-loaded guided tissue regeneration membranes. International Journal of Periodontics & Restorative Dentistry 2005; 25(3): 265-275.