論文名稱:

神經生長因子對酵素作用分離後的牙乳突間葉細胞之影響

 

The effects of nerve growth factor on the enzyme-separated dental papilla mesenchymal cells

研究生:

張宏羽  Hung-Yu Chang

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

楊世芳  Shih-Fang Yang

 

董醒任  Shing-Zeng Dung

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

          學年度:

86

          語文別:

中文

          出版年:

87

關鍵字:

神經生長因子  Nerve growth factor (NGF)

 

牙乳突  Dental papilla

 

間葉細胞  Mesenchymal cells

 

牙醫  DENTIST

 

牙醫學  DENTISTRY

 

口腔  ORAL-HEALTH

摘要:

牙齒發有的過程,是由外胚層的口腔上皮細胞與神經脊衍生女的外間葉細胞,兩者之間進行一連串複雜的交互作用,而共同完成。神經生長因子,已被證實在胚胎牙齒發育的初始期、形態發生期、細胞分化期,皆會出現於牙胚中。這篇論文的目的是為了觀察神經生長因子是否為間葉細胞分化造牙本質細胞的關鍵因子。我們在解剖顯微鏡下取出大白鼠下顎第一大白齒的牙胚,然後以蛋白酵素來分離並取得完整的牙乳突。將牙胚或牙乳突培養在含或不含神經生長因子的培養液中七天,再以Bouin溶液固定。在進行切片、H&E染色之後,以光學顯微鏡觀察,並比較牙乳突中間葉細胞的形態變化。實驗結果發現神經生長因子對於E15E17E19牙乳突間葉細胞有形態上的影響。神經生長因子處理過的牙乳突最外層細胞呈現類似造牙本質母細胞之短柱狀排列;次層細胞則有較為濃縮與聚集的情形出現。本研究結果顯示,在沒有牙上皮細胞的狀態下,神經生長因子可使牙乳突最外層未分化之間葉細胞趨向類似造牙本質細胞的分化。

   
 

Tooth development involves sequential and complex interactions between epithelial and mesenchymal tissue components which govern both tooth morphogenesis and cell differentiation.  Nerve growth factor (NGF) is found during all tooth development stages, including initiation, morphogenesis, and histodifferentiation.  The purpose of this project was to evaluate the impact ofNGF alone on odontoblasts differentiation from isolated mesenchymal dental papilla.  Tooth germs ofmandibular first molars from S.D. rats were obtained by gentle dissection. A novel protease was used to separate the intact dental papilla from dental epithelium.  Dental tissues including tooth germ, separated dental papilla were then incubated in 90% DMEM, 10% FBS, 1% penicillin-streptomycin and amphtotericin B with or without NGF 100 ng/m1for 7 days.  The collected dental tissues were fixed in Bouin's solution and processed for histological examinations. Results indicated that NGF has the non-neural effect for cell lining of outer dental papil-la at E15, E17 and E19.  The cellular shape of the outer layer became low columnar, and the cellular density was higher in the sub-outer layer.  In conclusion, NGF only could not proceed odontoblasts differentiation, while NGF may induce the outer cells of dental papilla to preodontoblastic lineage.