論文名稱:

牙科用合金經氮化膜覆鍍後生物相容性之研究

 

Biocompatibility of Dental Alloys after Nitride Film Coatings

研究生:

錢家琪  Chia-Chi Chien

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

鍾國雄  Kwok-Hung Chung

 

張國威  Kuo-Wei Chang

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

            學號:

39217001

          學年度:

93

          語文別:

中文

          出版年:

94

關鍵字:

牙科用合金  dental alloys

 

矽晶片  silicon wafer

 

氮化鈦薄膜覆鍍  titanium nitride film coating

 

氮化鋁鈦薄膜覆鍍  titanium-aluminum nitride film coating

 

生物相容性  biocompatibility

 

細胞毒性  cytotoxicity

 

皮下種植試驗  subcutaneous implantation test

全文說明:

(本論文未授權公開)

 

電子全文

        論文頁數:

105

摘要:

目的:一項嶄新的氮化鋁鈦(TiAlN)薄膜覆鍍技術已研發出並應用於覆鍍卑金屬合金以改進其表面生物相容性。本研究之的目的乃對經氮化膜覆鍍之試片進行細胞毒性測試和觀察卑金屬合金表面經覆鍍氮化膜在之皮下組織內之反應。

 

研究方法:細胞毒性試驗之矽晶片試片(10 mm × 10 mm × 1 mm)表面覆鍍氮化膜後使用舌癌鱗狀(SAS)細胞株、人類口腔角質細胞(NHOK)和人類口腔纖維母細胞(NHOF)進行測試,和利用MTT試劑配合免疫酵素分析儀進行細胞存活度的測量。細胞形態和細胞骨架則使用免疫螢光和掃瞄式電子顯微鏡進行觀察。另採用三種市售之牙科用合金製作成長條狀試片 (10mm × 3mm × 0.3mm ) ,在其表面進行氮化膜覆鍍。使用SD大鼠進行皮下組織種植試驗( ISO 10993 ),按標準處理步驟置入試片後,經4週、12週、26週和52週後觀察試片在皮下組織內之反應。以上各項試驗均進行三次,所得之各項數值採用變異數分析和Tukey多重比較試驗分析法判別介於覆鍍薄膜和未經覆鍍鍍薄膜各組間的差異性(p < 0.05)。

 

結果:舌癌鱗狀細胞株在MTT試驗所得的光密度值範圍自1.02 ± 0.171.84 ± 0.11,正常人類口腔角質細胞自0.45 ± 0.050.71 ± 0.06和正常人類口腔纖維母細胞則自1.57 ± 0.092.35 ± 0.23。舌癌鱗狀細胞株之細胞生長試驗比值為 6.55 ± 0.80,正常人類口腔角質細胞為 7.94 ± 1.74和正常人類口腔纖維母細胞為 2.76 ± 0.43。細胞毒性試驗結果顯示矽晶片覆鍍氮化鋁鈦薄膜後細胞存活度有顯著地增加(p < 0.05)和局部貼附增加現象。表面覆鍍氮化鋁鈦薄膜的金鈀合金產生的纖維囊的厚度範圍自7.49 ± 0.50m14.04 ± 0.37m,鈷鉻合金自6.30 ± 1.45m15.40 ± 0.50m 和鎳鉻合金自13.49 ± 0.67m 16.40 ± 0.50m。試片表面經覆鍍氮化鋁鈦薄膜均呈現較未經覆鍍薄膜之試片產生較薄的纖維囊 ( p < 0.05 )

 

結論:氮化鋁鈦薄膜具有良好且可增加卑金屬合金基材表面生物相容性的潛能。

   
 

Objective: A novel titanium aluminum nitride (TiAlN) film coating technique has been developed and applied on base metal alloys in order to improve their biocompatibility. The objectives of this study were to determine the cytotoxicity of nitride films and to observe the tissue reaction of base metal alloys after nitride film coating.

 

Methods: Cytotoxicity testing of silicon wafer specimen (10mm X 10mm X 1mm) surface coated with nitride films was performed using squamous cell carcinoma of tongue (SAS cell line) , normal human oral keratinocyte (NHOK), and normal human oral fibroblast (NHOF) with ELISA-based MTT assay to determine cellular viability. Cellular morphology and actin filament were observed using immunofluorescence and scanning electron microscopy.  Specimen strips (10mm X 3mm X 0.3mm) were fabricated with three commercially available dental alloys, and the surface was coated with nitride films accordingly. Subcutaneous tissue implantation test (ISO 10993) was conducted using SD rats for 4-week, 12-week, 26-week, and 52-week periods. Triplicate specimen in each group was used for all tests. An analysis of variance and Tukey multiple comparison test were performed to determine the differences in performance between before and after film coating groups statistically at p < 0.05 level.

 

Result: The optical density values of MTT assay of SAS cell line ranged from 1.02 ± 0.17 to 1.84 ± 0.11, NHOK from 0.45 ± 0.05 to 0.71 ± 0.06, and NHGF from 1.57 ± 0.09 to 2.35 ± 0.23. The cellular proliferation ratios obtained from SAS cell line was 6.55 ± 0.80, NHOK 7.94 ± 1.74, and NHGF 2.76 ± 0.43. The results of cytotoxicity test indicated that the silicon wafers coated with TiAlN film significantly increased cellular viability (p < 0.05) and local adhesion. The thickness of fibrous capsule adjacent to Au-Pd alloy surface coated with TiAlN film ranged from 7.49 ± 0.50m to 14.04 ± 0.37m, Co-Cr alloy from 6.30 ± 1.45m to 15.40 ± 0.50m, and Ni-Cr alloy from 13.49 ± 0.67m to 16.40 ± 0.50m. Specimen surfaces coated with TiAlN film produced significantly thinner fibrous capsule than uncoated surfaces (p < 0.05).

 

Conclusion: TiAlN film coating may have a good potential to increase the biocompatibility of base metal alloy substrate.