論文名稱:

神經生長因子對肢芽組織基質細胞之影響

 

The effects of nerve growth factor on limb bud derived stromal cells

研究生:

陳庭鵬  Ting-Peng Chen

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

楊世芳  Shin-Fang Yang

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

            學號:

38917005

          學年度:

90

          語文別:

中文

          出版年:

91

關鍵字:

神經生長因子  nerve growth factor

 

肢芽組織  limb bud

全文說明:

電子全文

        論文頁數:

60

摘要:

在胚胎發育的過程裡,體內各種具有不同功能的器官,都是來自於相同來源的細胞。這些器官的成型與分化,皆是由胚胎外、中、內三個胚層組織一連串的訊息傳遞以及彼此之間的交互作用所完成的。在調控器官成型的機制裡,其中最主要的就是上皮與間葉組織的交互作用,而在胚胎體內透過上皮與間葉組織交互作用成型的器官有很多,肢芽組織與牙胚組織的發育也是其中之一。已知神經生長因子,除了對神經脊衍生物包括感覺神經元及交感神經元的發育、分化與維持其存活是必須的之外,亦參與許多器官型態發生的過程,並在上皮與間葉組織間扮演重要的調控角色。過去,在以含有100ng/ml神經生長因子的細胞培養液與老鼠胚胎之牙間葉組織一起培養七天後,發現有部分細胞會從牙乳突組織中遷移出來,並且細胞的型態發生改變,分化成為型態類似神經細胞的類神經細胞,並以免疫細胞染色的方法證實,這些類神經細胞可以染上GFAPPeripherinneurofilament160等神經性標記。為了瞭解神經生長因子是否也可以促使非從神經脊組織所衍生而成的肢芽間葉組織細胞,改變其原有的命運,從非神經性的細胞分化成為神經性的細胞,故將胚胎第十二天、第十四天、第十六天之肢芽間葉組織細胞,以酵素作用將其分解為單一細胞,並將其培養於0ng/ml50ng/ml100ng/ml200ng/ml等不同濃度之神經生長因子細胞培養液七天,結果發現,從非神經性組織所衍生而成的胚胎肢芽間葉組織細胞,確實可以藉由神經生長因子的刺激,從非神經性的細胞分化成為神經性的細胞,並且隨著神經生長因子濃度的增加,影響細胞分化命運的能力也跟著增加。

   
   
 

Organs have different functions in the body all comes from the same germ cell in the process of embryonic development. Morphorgenesis and differentiation of these organs involves sequential and complex interaction between ectoderm, mesoderm and endoderm. The interaction between epithelial and mesenchymal tissue is the most important mechanisms of the organ morphegensis, and the development of limb bud and tooth germ is one of example suit to this model. Nerve growth factors (NGF) are known for the main key of development, differentiation and survival for sensory and motor neurons derived from the neural crest and also involve in the morphogenetic process of many organs. In the past, the separated dental papilla of S.D. rat was cultured in DMEM-serum medium with 100ng/ml NGF for 7days. Some cells migrated from the separated dental papilla were found and the cell types changed to neural-like cells identified by immnuohistochemistry with neural specific markers, like peripherin, glial fibrillary acidic protein and neurofilament-M. The purpose of this study was to evaluate if NGF would impact the differentiation of cell from non-neurogenic to neurogenic fate on limb bud derived stomal cells, so limb bud of the day 12, day 14, and day16 from the embyo of S.D.rat were used in experimental model. The limb bud mesenchymal tissue were digested by enzyme, and the cells were cultured in different concentration medium of NGF (0ng/ml50ng/ml100ng/ml200ng/ml) for 7 days. The results showed the limb bud mesenchymal stromal cells derived from non-neurogenic tissue would differetiate to neural-like cells in the stimulation of NGF and the relationship between NGF concentration and differentiation of cells' numbers have positive effect.