論文名稱:

神經標記物在牙胚發育的表現

 

Expression of neural markers in developing tooth

研究生:

柯信安  Hsin-An Ko

 

(以作者名查詢陽明大學館藏系統)

 

(以作者名查詢全國圖書書目資訊網)

指導教授:

楊世芳  Shih-Fang Yang

        學位類別:

碩士

        學校名稱:

國立陽明大學

系所名稱:

口腔生物研究所

            學號:

38917001

          學年度:

90

          語文別:

中文

          出版年:

91

關鍵字:

神經脊  Neural crest

 

牙乳突  dental papilla

 

造牙本質細胞  odontoblasts

 

免疫化學染色法  immunohistochemistry(IHC).

 

神經性標記  neural markers

摘要:

    神經脊細胞是具有多潛能的細胞,在脊椎動物的發育上形成多種形態的細胞及組織。在頭頸部可分化形成神經節中各種神經細胞及骨骼肌肉等結締組織與其各種衍生物如牙胚之牙乳突間各種細胞(應包括造牙本質細胞)。本實驗擬觀察此來自神經脊細胞的牙間葉組織細胞在分化過程中其神經性特質改變的情形。本實驗採SD品系大白鼠,以胚胎第十六、十七、十八、十九、二十、出生當天及出生後第四天的牙胚為實驗對象,取出牙胚組織後經過固定、石蠟包埋、連續切片,再以免疫化學染色法(IHC)觀察神經細胞發育各個時期中所表現的神經性標記,包括:NestinSynapsinSynaptotagminNeu NNSEA2B5GFAPS100。實驗結果顯示較早期的神經性標記,如Nestin,可表現在分化中之牙間葉組織細胞,但分化階段不同其表現情況有所差異。較晚期的神經性標記,如:A2B5NSE,其在造牙本質細胞分化過程中表現逐漸增強。在牙胚發育過程中的牙間葉組織細胞則無法觀察到其他的神經性標記,如SynapsinSynaptotagminGFAPS100。根據本實驗的結果推測部分牙間葉組織細胞進行終末分化後,仍然保有某些神經特質。

   
 

Abstract Neural crest cells are multipotential cells that contribute extensively to vertebrate development and give rise to various cell and tissue types. In the region of head and neck formation, neural crest cells contribute to the formation of arch nerve ganglia, bones, cartilage, and connective tissues, other neural crest-derive cells, eg dental papilla cells should include odontoblasts. In the present study we examined the in vivo distribution of neural markers in SD-rat teeth during serial tooth development stagesE16, E17, E18, E19, E20, p0, p4. After the tooth generated, we embedded tooth germs in paraffin and paraffin blocks cut into serial sections, then we observed the expression of neural markersNestinSynapsinSynaptotagminNeu NNSEA2B5GFAPS100by immunohistochemistryIHC. Our results indicate that the early neural differentiation marker,“ Nestin ” expresses in the dental papilla cells and increases from the apical to the cusp area following the differentiation gradient. The neural markers of neural cells’ terminal differentiation eg, NSE and A2B5, these expression in developing tooth seems following the maturity of odontoblasts. Other neural markers eg, Synapsin, Synaptotagmin, GFAP, and S100, can not be observed in each stage of dental papilla cells’ differentiation. According to these results we suggest that after dental papilla cells differentiated, they still maintain some neural characteristic.