Dr. Wen-Cherng Tsai, Professor

Phone: 886-2-2826-7110
E-mail: wctsai@ym.edu.tw
Education Research Publications


1971, M.S.  Department of Bacteriology and Public Health
       Utah State University, Logan, Utah, USA

1973 - 1974 Diploma
       School of Medical Technology, Idaho Falls Hospital, Idaho Falls, Idaho

1976, Ph.D. Department of Microbiology
       South Dakota State University, Brookings, SD, USA

1976 - 1977 Post-doctorate fellow
       Department of Chemistry, Arlington, Texas, USA


MT (ASCP) American Society for Clinical Pathologists, USA

RM (AAM) Registered Microbiologist, American Association for Microbiology, USA

CLT (California) Clinical Laboratory Technologist, California, USA


1. Evaluation the effectiveness of ANA-PRESUMPTO computer coding system.

2. Rapid identification of Staphylococcus epidermidis by desferrioxamine susceptibility and trehalose fermentation tests.

3. Evaluation a simple method to detect the Legionella spp. present in cooling tower of air-conditioning.

4. Some Factors influencing the pyocyanine-producing capability of Pseudomonas aeruginosa growing in Mueller-Hinton agar.

5. Compare the effectiveness of different brands of TCBS medium for growing Vibrio vulnificus.

6. Some factors influencing the production of indo-pyruvic acid from SIM medium.

7. Design a practical and effective method for disinfecting the requestion forms in Clinical Microbiology Laboratories.


1. Tsai WC, Ho MC. 1996. GFB-14E: A computer-coding system for the conventional identification of enteric bacteria and other glucose-fermenting gram- negative bacilli. pp.1-1103. Taipei: Giou-Chow Publication Co.

2. Tsai WC, Ho MC. 1996. GNF-18 IDEN Computer-coding system for the identification of Glucose-nonfermenting gram-negative bacilli. pp.1-952. Taipei: Giou-Chow Publication Co.

3. Tsai WC, Ho MC. 1996. ANA-Presumpto computer-coding system for the identification of anaerobic bacteria. pp. 1-267. Taipei: Giou-Chow Publication Co.

4. 蔡文城。1996。水中大腸菌群之簡便偵測與鑑定方法。於楊盛行(主編):食品微生物污染與檢測方法。台北:中華民國微生物學會。71-80。

5. Wu J-J, Chien M-L, Lee N, Chou S-F, Wang H-M, Tsai W-C. 1996. Microbiologic assay for detection of antimicrobial agents in urine. J Formos Med Assoc. 95:464-68.

6.Tsai W-C, Wu J-L, Luh K-T. 1995. Clinical microbiology quality assurance program: a Taiwan experience. Chinese J Microbiol Immunol. 28:95-108.

7. 蔡文城。1995。從圖書館內空氣品質的影響因素談如何維護館內優良空氣品 質。圖書館管理學報。1:21-34。

8. 蔡文城、顧蕙祺。1995。圖書館空氣微生物總含量分析及其影響因素。中國圖書館學會會報。54:5-12。

9. Huang M-Z, Lee J-H, Liu S-T, Tsai W-C. 1994. Light-emitting Aeromonas and Plesiomonas by transconjugating luxAB from Escherichia coli. Chin. J. Microbiol. Immunol. 27:206-210.

10. 李家華、黃苗政、吳竹蘭、蔡文城。1994。評估新的四小時腸內菌鑑定套組 RapIDonE 系統。中華微免雜誌。27:133-139。

11. Lin M-Y, Cheng M-C, Huang K-J, Tsai W-C. 1993. Classification, pathogenicity, and drug susceptibility of hemolytic gram-negative bacteria isolated from sick or dead chickens. Avian Dis. 37:6-9.

12. Yeh TJ, Tsai WC. 1991. Plesiomonas shigelloides-associated diarrhea. Chin. Med. J. (Taipei) 47:362-368.

13. Lai HC, Tsai WC. 1990. Antibiotic combination effect on the clinical isolates of Aeromonas hydrophila. Chin. J. Microbiol. Immunol. 23:44-52.

14. Liou JJ, Lin LY, Juane RF, Chang NC, Tasi WC, Hu ST. 1990. Cloning and sequencing of hemolysin gene from clinical Aeromonas hydrophila, Chin. J. Microbiol. Immunol. 23:134-146.

15. Yeh JJ, Chin TW, Tsai WC, Wei CF. 1990. Mucosal intussusception to avoid ascending cholangitis. Br. J. Surg. 77:989-991.

16. Lai HC, Hu ST, Liu ST, Tsai WC. 1990. Clinical Aeromonas hydrophila contain the DNA fragments with nucleotide sequence similar to those of hemolysin and cholera-toxin gene. Chin. J. Microbiol. Immunol. 23: 126-133.

17. Chen SC, Tung MC, Tsai Wc. 1989. An epizzootic in Formosa snake-head fish (Channa maculata Lacepede), caused by Nocardia asteroides in fresh water pond in southern Taiwan. Fish Disease Research (IX) (6):42-48.

18. Tsai WC, Wu JL, Wang JJ, Wang AM, Lee N, Chou SF. 1989. The ratios and kinds of clinical bacteria isolated in Taiwan's large-size hospitals. Chin J. Microbiol Immunol. 22:46-58.

19. King CC, Chen CJ, You SL, Chuang YC, Huang HH. Tsai WC. 1989. Community-wide epidemiological investigation of a typhoid outbreak in a rural township in Taiwan, Republic of China. Internat. J. Epidemiol. 18:254-260.

20. Chong KY, Tseng CP, Tsai WC.1989. The susceptibility patterns of fifteen common clinical bacteria against different cephalosporins. J. Bimed. Lab. Sci. 2:12- 23.

21. Tsai WC, Wu JL, Wang JJ, Wang AM, Lee N, Chou SF. 1989. The ratio and kinds of clinical bacteria isolated in Taiwan's large-size hospitals. Chin. J. Microbiol. Immunol. 22:46-58.

22. Tsai WC, Chen CL, Liou CM, Chow SF, Yu KW, Liu CY, Cheng DL. 1988. Detect the polymicrobial bacteremia by a modified subculturing technique of blood culture. Chin. Med. J. (Taipei) 42:151-156.

23. Tsai WC, Tarng CM, King J, Ko CD, Wu HJ. 1988. Evaluation on the capability of indole pyruvic acid production by members of Proteeae on different brands of sulfide-indole-motility media. 21:117-124.

24. Wu SJ, Chow SF, Li SN, Tsai WC. 1988. A modified medium, bile esculin- pyrolidonyi-beta-naphthylamide, for rapid differentiation between enterococci and nonenterococci. Chin. J. Microbiol. Immunol. 21:88-190.

25. Shen HD, Choo KB, Tsai WC, Jen TM, Yeh JY, Han SH. 1988. Differential denitrification of Candida species and other yeasts by analysis of (35S) methionine- labeled polypeptide profiles. Analytical Biochem. 175:548-551.

26. Duh RW, Wang LS, Liu CY, Cheng DL, Wang WW, Tsai WC. 1988. Haemophilus bacteremia in adults. JIDS. ROC. 1:25-28.

27. Yu LW, Liu CY, Tsai ST, Tsai WC, Wang LS, Cheng DL. 1988. Listeria monocytogenes in systemic lupus erythematosus: A case report. JIDS, ROC. 1:59- 62.

28. Tsai WC, Chu SH, Lee SY, Lee PF. 1987. The effect of different brands of MacConkey and xylose lysine deoxycholate agar on isolation of Aeromonas hydrophila. Chin. J. Microbiol. Immunol. 20:257-261.

29. Chu CC, Tsai WC, Yao JY, Chiu SS. 1987. Newly made antibacterial braided nylon sutures. I. In vitro qualitative and in vivo preliminary biocompatibility study. J Biomed. Mater. Res. 21:1281-1300.

30. Tsai WC, Chu CC, Chiu SS, Yao JU. 1987. In vitro quantitative study of newly made antibacterial braided nylon sutrues. Surgery, Gynecology & Obstetrics. 165;207-211.

31. Tsai WC, Chow SF. 1987. Urinary tract infections and Microbiological characteristics of Proteus penneri isolated in Taiwan. Chin. J. Microbiol. Immunol. 20:132-139.

32. Tsai WC, Chen HL, Yang CS, Chin YH. 1987. GFB- 12E computer-coding bacterial identification system. Taipei: Jeou-Chou Book Co. pp. 1-333.

33. Chen HL, Chin YH, Tsai WC. 1987. KID: An expert system to identify Gram- negative bacilli. Proceeding of national computer symposium. P. 235-240.

34. Tsai WC. 1987. Case report of Listeria monocytogenes infection in Taiwan and the method to isolate this organisms from heavily contaminated environments. Proceeding of Food and Drug Safety symposium sponsored by Bureau of Food and Drug Administration, Taiwan. ROC. P. 235-240.

35. Wu SH, Lin SJ, Tso HM, Liu CB, Tsai WC. 1986. Fatal septicemia due to Chromobacterium violaceum. Chin. J. Microbiol. Immunol. 19:289-294.

36. Tsai WC, Lee H, Ju YC. 1986. Comparative study of the biotype, hemolysin- producing capability and antibiorgram of the aquatic and the clinical strains of Aeromonas hydrophila. Chin. Microbiol. Immunol. 19:373-386.

37. Lee N, Wu JL, Lee CH, Tsai WC. 1985. Pseudomonas pseudomallei infection from drowning: the first reported case in Taiwan. J. Clin. Microbiol. 23:352-354.

38. Wu JS, Wu SM, Yih P, Tsai WC. 1985. Microbiological examination of foods sold via vending machines. Chin. J. Microbiol. Immunol. 18:135-142.

39. Lee N, Wu JN, Tsai WC. 1985. Fungemia: A clinical and pathological review of 16 cases. Chin. Med. J. 35:265-272.

40. Chen LL, Lee N, Wu JL, Tsai WC. 1985. A simple method for performing carbohydrate assimilation test. Chin. J. Microbiol. Immunol. 18:282-287.

41. Tsai WC, Wu MY, Lee RH. 1984. Evaluation on the significance of the establishment of a microbiological laboratory in the flight kitchen of developing countries. Chin. Aero. Med. J. pp. 15-21.

42. Wu JL, Lee N, Tsai WC. 1984. Effect of the types and atmosphere of culture media on isolation of microorganisms from blood cultures. Chin. Med. J. 33:176- 181.

43. Lien SL, Tang RB, Wang HC, Tsai EC. 1984. Frequencies of occurrence of the diarrhea-causing pathogenic bacteria in patients of the pediatric out-patient department. Chin. Med. J. 33:331-340.

44. Tsai WC, Lee MF, Tarng CM. 1983. GNF computer-coding system for identifying glucose-nonfermenting Gram-negative bacilli. Taipei: Ho-Chi book Co. pp.1-381.

45. Tarng CM, Chen MSG, Tsai WC. 1983. Evaluation of the GNF computer-coding system for identification of non-fermentative Gram-negative bacilli. Chin. J. Microbiol. Imunol. 16:31-43.

46. Chang CY, Shen LY, Tsai WC. 1983. The Microbial contents of air surface sampling in surgical rooms with particular reference to its influencing factors. Chin. J. Surgery. 16:227-235.

47. Tsai WC, Wong CL. 1982. The Microbial contents of public telephone receivers and the antibiograms of staphylococcal isolates. Proc. Natl Sci. Counc. ROC 10:870-878.

48. Tsai WC, Chang C, Tarng CM. 1982. Lecthinase activity of Pseudomonas aeruginosa isolation in Taiwan. Chin. J. Microbiol. Immunol. 15:253-254.

49. Tsai WC. Lee MF. Tarng CM. Lung LG. Shen H. Jen TM. Chen KY. 1982. ERA- 18NF Multaitest kit and its computer-coding identification system. Taipei: Jeou- Chou Book Co. pp.1-761.

50. Tsai WC, Chen CY, Chen DL. 1981. A multi-tube micro-system for rapid identification of Enterobacteriaceae. Proc. Natl. Sci. Counc. ROC. 5:111-118.

51. Tsai WC, Liu LJ, Chen CY. 1981. Aeromonas hydrophila infection in a patient with pulmonary tuberculosis. Chin. Med. J. 28:197-201.

52. Tsai WC, Ho SW, Lee MF, Chen CY, Sun TL. 1981. YM-NTUH computer coding system for identifying glucose-fermenting Gram-negative bacilli. Taipei: Ho- Chi Book Co. pp.1-193.

53. Chen C Y, Tsai WC, Ho SW. 1981. Evaluation of the effectiveness of the YM- NTUH computer-coding system in identifying glucose-fermenting Gram-negative bacilli. Chin. Med. J. 28:52-58.

54. Tsai WC, Wu JJ. 1979. Serotypes and biotypes and antibiotic susceptibility of Haemophilus influenzae encountered in a clinical laboratory in Taiwan. Chin. J. Microbiol. 12:140-148.

55. Tsai WC, Westby CA. 1978. Synthesis and salvage of purines during the cellular morphogenesis of Myxococcus xanthus. J. Bacteriol. 136:582-587.

56. Tsai WC. 1976. Purine-thiamine anabolism during cellular morphogenesis in Myxococcus xanthus. Ph. D. Dissertation. South Dakota State University.

57. Westby CA, Tsai WC. 1974. De novo purine synthesis in vegetative cells and myxospores of Myxococcus xanthus. J. Bact. 177: 1099-1107.

Members of Laboratory