Dr. Ling-Pai Ting, Professor

Phone: 886-2-2826-7106
E-mail: lpting@ym.edu.tw
Education@Research@Publications


Education

1974, B.S.@ Medical Technology
@@@@@@ National Taiwan University, Taiwan

1976, M.S.@ Biochemistry
@@@@@@ National Taiwan University, Taiwan

1980, Ph.D.@Biochemistry
@@@@@@ State University of New York at Buffalo, USA

1980 - 1981@Research Associate
@@@@@@ Einstein Chair Laboratory, State University of New York at Buffalo, USA

1981 - 1981@Research Instructor
@@@@@@ Einstein Chair Laboratory, State University of New York at Buffalo, USA

1981 - 1985@Associate Professor
@@@@@@ Department of Medical Technology, National Yang-Ming University, Taiwan

1981 - 1987@Associate Professor
@@@@@@ Institute of Microbiology & Immunology, National Yang-Ming University, Taiwan

1991 - 1996@Director
@@@@@@ Institute of Microbiology & Immunology, National Yang-Ming University, Taiwan

1987 -@@@ Professor
@@@@@@ Institute of Microbiology & Immunology, National Yang-Ming University, Taiwan

1985 - 1985@Visiting Scientist
@@@@@@ Cold Spring Harbor Laboratory, USA

1996 - 1997@Visiting Professor
@@@@@@ UCLA, Los Angeles, USA

1987@@@@ Research Chair Award of The Tsing-Ling Medical Foundation

1990@@@@ Award of Liver Disease Prevention & Treatment Research Foundation

1992@@@@ Award of The Cathy Foundation Research

1986 - 1990@The Excellent Research Award of National Science Council

1994 - 1995@The Excellent Research Award of National Science Council

1990 - 1992@The Outstanding Research Award of National Science Council

1992 - 1994@The Outstanding Research Award of National Science Council

1995 - 1997@The Outstanding Research Award of National Science Council

1997 - 2000@The Outstanding Research Chair of National Science Council

2000 - 2003@The Outstanding Research Chair of National Science Council

1999@@@@ The Academic Outstanding Award of Education Department

1993 - 2001@Member of Board of Governors
@@@@@@ The Chinese Society of Microbiology

1995-@@@@Member of Board of Governors
@@@@@@ The Chinese Society of Cell and Molecular Biology

1999-@@@@Member of Board of Governors
@@@@@@ The Taiwan Society for Biochemistry and Molecular Biology

1999-@@@@Associate Editor
@@@@@@ Journal of Biomedical Science


Research

@@The long-term research interest of this laboratory is the transcriptional regulation of hepatitis B viral (HBV) genes, and cloning and characterization of cellular transcription factors involved in the regulation of HBV genes. The present focus is on the negative regulatory element (NRE) of HBV. We have cloned the cDNA encoding the binding protein of NRE, NREBP. NREBP has been mapped in a critical region for an autosomal dominant familial platelet disorder associated with development of acute myelogenous leukemia and in close proximity to the Down syndrome critical region. The functional domains, associated proteins, and repression mechanism of NREBP will be identified and characterized. Furthermore, the cellular target genes of NREBP and their possible relation to the human disease will be studied. Regulation of NREBP gene expression will also be studied to further understand its physiological role.

@@A recent research interest is to identify cellular proteins which interact with the HBV core protein. Several cDNAs encoding proteins involved in the signal transduction pathways have been identified. The interaction between HBV core protein and these cellular proteins, the effect of these proteins on the viral replication and pathogenesis, and the effect of HBV core protein on the cell physiology will be analyzed.


Experimental Techniques

@@The experimental techniques performed in this lab: Plasmid construction, DNA sequencing, PCR, RT-PCR, Southern and Northern hybridization, S1 mapping, Primer extension, In vitro transcription, Preparation of nuclear extract, DNaseI footprinting, Gel shift (EMSA), Transfection, CAT assay, Luciferase assay, cDNA & genomic library screening, Expression & purification of recombinant protein, Antibody generation &purification, Western, Immuno-precipitation, Immuno-fluorescence, Confocal microscope, Southwestern (Filter binding assay), Far western, Kinase assay, In gel kinase, Yeast two hybrid screening, Receptor binding, etc.


Publications

1. Ting, L. P., C. L. Tu, and C. K. Chou. 1989. Insulin-induced expression of human heat shock protein gene hsp70. J. Biol. Chem. 264: 3404-3408.

2. Chang, H. K., and L. P. Ting. 1989. The HBsAg and HBcAg promoter regions of hepatitis B virus display the differentiated hepatocyte preference. Virol. 170: 176-183.

3. Chang, H. K., B. Y. Wang, C. H. Yuh, C. L. Wei, and L. P. Ting. 1989. A liver-specific nuclear factor interacts with the promoter region of the large surface protein gene of the human hepatitis B virus. Mol. Cell. Biol. 9: 5189-5197.

4. Yuh, C. H., and L. P. Ting. 1990. The genome of the hepatitis B virus contains a second enhancer: cooperation of two elements within this enhancer is required for its function. J. Virol. 64: 4281-4287.

5. Yuh, C. H., and L. P. Ting. 1991.C/EBP-like proteins binding to the functional box-a and box-b of the second enhancer of hepatitis B virus. Mol. Cell. Biol. 11: 5044-5052.

6. Wu, J. C., P. J. Chen, M. Y. P. Kuo, S. D. Lee, D. S. Chen, and L. P. Ting. 1991. Production of hepatitis D virus and suppression of helper hepatitis B virus in a human hepatoma cell line. J. Virol. 65: 1099-1104.

7. Yuh, C. H., Y. L. Chang, and L. P. Ting. 1992. Transcriptional regulation of precore and pregenomic RNAs of hepatitis B virus. J. Virol. 66: 4073-4084.

8. Yuh, C. H., and L. P. Ting. 1993. Differentiated liver-cell specificity of the second enhancer of hepatitis B virus. J. Virol. 67: 142-149.

9. Lo, W. Y., and L. P. Ting. 1994. Repression of enhancer II activity by a negative regulatory element in hepatitis B virus genome. J. Virol. 68: 1758-1764.

10. Chen, I. H., C. J. Huang, and L. P. Ting. 1995. Overlapping initiator and TATA box functions in the basal core promoter of hepatitis B virus. J. Virol. 69: 3647-3657.

11. Kuo, Y. L., and L. P. Ting. 1997. Differential regulation of major surface promoter in hepatitis B virus. DNA and Cell Biol. 16: 545-557.

12. Kau, J. H., and L. P. Ting. 1998. Phosphorylation of core protein of hepatitis B virus by a 46-kDa serinekinase. J. Virol. 72: 3796-3803.

13. Lai, C. K., and L. P. Ting. 1999. Transcriptional repression of human hepatitis B viral genes by a bZIP family member, E4BP4. J. Virol. 73: 3197-3209.

14. Huang, C. J., Y. H. Chen, and L. P. Ting. 2000. Hepatitis B virus core protein interacts with the C-terminal region of actin-binding protein. J. Biomed. Sci. 7: 160-168.

15. Sun, C. T., W. Y. Lo, I. H. Wang, Y. H. Lo, S. R. Shiou, C. K. Lai, and L. P. Ting. 2001. Transcription repression of human hepatitis B virus genes by negative regulatory elements-binding protein/ SON. J. Biol. Chem. 276: 24059-24067.


Members of Laboratory