Chen CW, Yu T-W, Chung H-M, Chou CF

Discovery and characterization of a new transposable element Tn4811 in Streptomyces lividans 66

Journal of Bacteriology 174: 7762-7769 (1992)

ABSTRACT

Transposition of a new 5.4-kb transposon Tn4811 of Streptomyces lividans to the melC operon of Streptomyces antibioticus on plasmid pIJ702 was discovered. The nt sequence of this copy of Tn4811 was determined, which contained an imperfect (9 out of 11 bp) terminal inverted repeat and five putative Streptomyces coding sequences for a oxidoreductase and its transcription regulator, and three transposition-related proteins. SLP^- strains of S. lividans contained one copy (A) of Tn4811, while SLP2⁺ strains contained an additional copy (B) on the SLP2 plasmid. The nt sequences at three insertion junctions of Tn4811 were determined. Copy B lacked 41 bp from the left end. At the other five junctions the duplication of a putative 3-bp target sequences (TGA) was observed. A sequence of less than 3 kb homologous to Tn4811 was present in S. antibioticus. DNA homologous to Tn4811 was not detected in 14 other Streptomyces species.