Biochemistry, University of Tennessee
often targets critical regulatory events in host cells. The effects of
viruses on the host cells can be mediated by addition or substitution of
virus-specific macromolecules to a cellular complex. Alternatively, the
virus may cause a disassembly or rearrangement of a host-cell complex, or
lead to the assembly of a new infected cell-specific complex. Thus,
knowledge of the processes subverted by viruses has often highlighted the
mechanisms of viral pathogenesis. The main goal of our study is to elaborate
the types of interactions between hepatitis virus-encoded macromolecules and
the host cells, which may define the ultimate outcome of a virus infection.
You, L.R., Hwang, L.H., and Lee, Y.H.W. (1997). Direct
interaction of hepatitis C virus core protein with the cellular
receptor modulates the signal pathway of the
receptor. J. Virology 71, 9417-9426.
T.S., and Lee, Y.H.W. (1998). Assembly of hepatitis delta virus
particles: package of multimeric HDV genomic
RNA and role of phosphorylation. Virology
Chen, C.M., and Lee, Y.H.W. (1999). The hepatitis C virus core
protein modulates the NF-kB
signal pathway triggering by lymphotoxin-b
receptor ligands and tumor necrosis factor-a.
J. Virology 73, 1672-1681.
Chen, C.M., Yeh, T.S., Tsai, T.Y., Mai, R.T.,
Lin, C.H., and Lee, Y.H.W. (1999). Hepatitis C virus core protein
interacts with cellular putative RNA helicase.
J. Virology 73, 2841-2853.
Lee, Y.H.W., Yen, S.H., and Chi, K.W. (2000). A novel approach
for nasopharyngeal carcinoma treatment uses
phenylbutyrate as a protein kinase c
modulator: implications for radiosensitization
and EBV-targeted theraphy.
Clin. Cancer Res. 6, 1452-1458.
Yung, B.Y.M., Syu, W.J., and Lee, Y.H.W.
(2001). The nucleolar
phosphoprotein B23 interacts with hepatitis delta antigen and
modulates the hepatitis delta virus RNA replication. J. Biol. Chem. 276,
Kao, C.F., Chen, C.M., Shih, C.M., Hsu, M.J., Chao, C.H. C., Wang, S.H.,
You, L.R., and Lee, Y.H.W. (2003). Mechanisms for inhibition of
hepatitis B virus gene expression and replication by hepatitis C virus
core protein. J. Biol. Chem. 278, 591-607.
Chen, S.Y., Chen, J.Y., Lee, Y.H.W. (2004) Modulation of p53
transcription regulatory activity and posttranslational modification by
hepatitis C virus core protein. Oncogene 23,
Chen, S.Y., Lee, Y.H.W. (2004) Activation of RNA polymerase I
transcription by hepatitis C virus core protein. J.
Chang, M.H., Chao, C.H., Lee, Y.H.W. (2004) Hepatitis C viral
proteins interact with Smad3 and differentially regulate the TGF-b/Smad3-mediated
transcriptional activation. Oncogene 23,
Yeh, T.S., Huang, H.H.,
Sun, S.K. and Lee, Y.H.W. (2006) Hepatitis C virus core
protein recruits nucleolar
phosphoprotein B23 and
coactivator p300 to relieve the repression effect of
transcription factor YY1 on B23 gene expression.
Oncogene 25, 448-462.
Chang, P.C., Chi, C.W.,
Chau, G.Y., Li, F.Y., Tsai, Y.H., Wu, J.C.,
and Lee, Y.H.W. (2006) DDX3. a DEAD
box RNA helicase, is deregulated in
hepatitis virus associated hepatocellular
carcinoma and is involved in cell growth control.
Oncogene 25, 1991-2003.
Chao, C.H., Chen, C.M.,
Cheng, P.L., Shih, J.W., Tsou, A.P., and
Lee, Y.H.W. (2006) DDX3, A DEAD box RNA
helicase with tumor growth-suppressive property and
transcriptional regulation activity of the p21waf1/cip1 promoter, is a
candidate tumor suppressor. Cancer Research 66(13):6579-6588
Shih, J.-W., T.-Y. Tsai, and Y.-H. W. Lee*. Candidate tumor
suppressor DDX3 RNA helicase specifically represses cap-dependent
translation by acting as an eIF4E inhibitory protein. Oncogene
Huang, W.H., Mai, R.T., and
Lee, Y.H.W. The transcription factor YY1 and its associated
acetyl-transferases CBP and p300 interact
with hepatitis delta antigens and modulates the hepatitis delta virus
RNA replication (manuscript in preparation).