PhD. Biochemistry, National Yang-Ming University
Post-Doc, Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
My current work and future goals seek to better understand the genetic basis of cardiovascular development using mouse genetic models. The most immediate goal will be the continued utilization of mouse genetics, molecular biology and biochemical approaches to further reveal the function of COUP-TFII and the genetic pathways through which it acts to control heart development. My long-term goals include exploration novel genes, which are functional associated with cardiovascular development, elucidation of the genetic networks and underlying mechanism. The mechanisms that underlie the morphogenic processes forming a heart have important implications not only for understanding congenital heart disease, but also for the possibility of cardiac repair. Eventually this knowledge will enable us to improve and prevent the corresponding diseases.
1. S. J. Lo, L. -R. You and Y. -H. Wu Lee. (1990) The hepatitis B virus X-C fusion protein is unlikely to be produced by the mechanism of ribosomal frameshifting. Virology 178, 584-587.
2. C. G. Lin, L. -R. You and S. J. Lo. (1993) Expression and stability of the hepatitis B pol protein in Escherichia coli. J. Gestroentrology and Hepatology 8, S54-58.
3. C. –M. Chen*, L. -R. You*, L. -H. Hwang and Y. -H. Wu Lee. (1997) Direct interaction of hepatitis C virus core protein with the cellular lymphotoxin-beta receptor modulates the signal pathway of the lymphotoxin-b receptor. J. Virol 71, 9417-9426. ( * equal contribution)
4. L. -R. You, C. -M. Chen and Y. -H. Wu Lee. (1999) The hepatitis C virus core protein modulates the NF-kB signal pathway triggering by lymphotoxin-beta receptor ligand and tumor necrosis factor-alpha. J. Virol 73(2), 1672-1681.
5. L. -R. You, C. -M. Chen, T. -S. Yeh, T, -Y. Tsai, R. -T. Mai and Y. -H. Wu Lee (1999) Hepatitis C virus core protein interacts with cellular putativeRNA helicase. J. Virol 73(4), 2841-2853.
6. T. J. Jaw, L. –R. You, P. S. Konepfler, L. -C. Yao, C. -Y. Pai, C. -Y. Tang, , L. -P. Chang, Berthelsen, J., Kamps, M. P. and Y. H. Sun. (2000) Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. Mech. Dev. 91(1-2), 279-91.
7. S. -Y. Chen, C. -F. Kao, C. -M. Chen, C. -M. Shih, M. -J. Hsu, C. -H. Chao, S, -H. Wang, L. -R. You and Y. -H. Wu Lee. (2003) Mechanisms for inhibition of hepatitis B virus gene expression and replication by hepatitis C virus core protein. J. Biol Chem. 278(1), 591-607.
8. N. Takamoto , L. -R. You, K. Moses, C. Chiang, W. E. Zimmer .. R. J. Schwartz, F. J. DeMayo, M. -J. Tsai and S. Y. Tsai. (2005) COUP-TFII is essential for radial and anterior-posterior patterning of the stomach. Development 132, 2179-2189.
9. L. -R. You, F. -J. Lin, C. T. Lee, F. J. DeMayo, M. –J. Tsai and S. Y. Tsai. (2005) Suppression of Notch signalling by the COUP-TFII transcription factor regulates vein identity. Nature 435, 98-104.
11. L. -R. You, N. Takamoto , C. -T. Yu, T. Tanaka, T. Kodama, F. J. DeMayo, S. Y. Tsai and M. -J. Tsai. (2005) Mouse lacking COUP-TFII as an animal model of Bochdalek-type congenital diaphragmatic hernia. Proc Natl Acad Sci U S A. 102(45): 16351-16356.
12. L. -R You, M. –J. Tsai and S. Y. Tsai. Nuclear orphan receptor, COUP-TFII, is essential for heart development. (Manuscript in preparation)