Kin-Fu Chak

   PhD. Molecular Biology, University of East Anglia, UK

Research

  1. Bio-molecular studies of the mechanism for spinal cord regeneration.
  2. Mechanism of translocation and secretion processes of colicin across the membrane of E. coli.
  3. Establishment of a proteomic platform technology for the investigation of biomarkers related to the efficacy of some selected Chinese herbal medicines.

Publications

  1. Ko T-P, Liao C-C, Ku W-Y, Chak K-F* and Yuan H* (1999) The crystal structure of the endomuclease domain of colicin E7 in complex with its inhibitor Im protein. Structure, 7:91-102.

  2. Lu F. M., Yuan, H., Cheng S. J and Chak K-F.* 1999. Hierarchy of critical residues for specific immunity on the immunity determining region of the Im7 protein. Biochemical and Biophysical Research Communications, 264, 69-75.

  3. Kuo W. S. and Chak K-F.* 2000. Characterization of two new cry-type genes from Bacillus thuringiensis and the cry-type genes organization of the Bt strain. Current Microbiology, 40, 227-232.

  4. Liao C. C., Hsiao K. C., Liu Y. W., Liang P. H., H. S. Yuan and Chak K-F.* 2001. Processing of DNase domain during translocation of colicin E7 across the membrane of Escherichia coli. Biochemical and Biophysical Research Communications, 284, 556-562.

  5. Ku W.Y., Liu Y.W., Hsu Y.C., Liao C.C., Liang P.H.,Yuan H.S. and Chak K-F*. 2002. The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis. Nucleic Acids Research 30, 1670-1678.

  6. Chang S.J., Hsieh S.J., Yuan H.S. and Chak K-F.* 2002. Characterization of the specific cleavage of ceiE7-mRNA of the bactericidal ColE7 operon. Biochemical and Biophysical Research Communication, 299, 613-620.

  7. Cheng Y-S., Hsia K-C., Doudeva L-G., Chak K-F. and Yuan H.S. 2002. The crystal structure of the nuclease domain of colicin E7 suggests a mechanism for binding to double-stranded DNA by the H-N-H endonucleases. Journal of Molecular Biology, 324, 227-236.

  8. Chen F-C., Shen L-F., Tsai M-C. and Chak K-F*. 2003. The IspA protease's involvement in the regulation of the sporulation process of Bacillus thuringiensis is revealed by proteomic approach. Biochemical and Biophysical Research Communications, 312, 708-715.

  9. Hsia K-C., Chak K-F., Liang P-H., Cheng Y-S., Ku W-Y. and Yuan H. S*. 2004. DNA binding and degradation by the HNH protein ColE7. Structure, 12, 205-214.

  10. Lin Y-H., Liao C-C., Liang P-H., Yuan H.S. and Chak K-F. 2004. Involvement of colicin in the limited protection of the colicin producing cells against bacteriophage. Biochemical and Biophysical Research Communication, 318, 81-87.

  11. Chen F-C., Shen L-F. and Chak K-F.* 2004. A facile analytical method for the identification of protease gene profiles from Bacillus thuringiensis strains. Journal of Microbiological Methods, 56, 125-132.

  12. Chen F-C., Tsai M-C., Peng C-H. and Chak K-F.* 2004. Dissection of cry gene profiles of Bacillus thuringiensis isolates in Taiwan. Current Microbiology, 48, 270-275.

  13. Shi J-H., Chak K-F.,* Yuan HS. 2005. Identification of an essential cleavage site in ColE7 required for import and killing of cells. Journal of Biological Chemistry, 280, 24663-24668.

  14. Cheng Y-S., Shi Z-H., Doudeva LG., Yang W-Z., Chak K-F.,* Yuan HS. 2006. High-resolution crystal structure of a truncated ColE7 translocation domain: Implications for colicin transport across membranes. Journal of Molecular Biology. 356 (1), 22-31.

  15. Pan Y-H., Liao C-C., Duan K-J., Liang P-H., Yuan HS., Hu S-T., Chak K-F.* 2006. The critical roles of polyamines in regulating ColE7 production and restricting ColE7 uptake of the colicin producing Escherichia coli.Journal of Biological Chemistry. 2006; 281(19):13083-13091.

  16. Yuan-Ya Lee, Shiau-Ting Hu, Po-Huang Liang, Kin-Fu Chak. An E.coli lon mutant conferring partial resistance to colicin may reveal a novel role in regulating proteins involved in the translocation of colicin. Biochemical and Biophysical Research Communications.2006;345:1579-1585

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