PhD. Chemistry, Indiana University
Our laboratory is working on the molecular mechanisms of p53 action. p53 tumor suppressor functions as a guardian of the genome that plays an important role in suppressing cancer development, and mutation on the p53 gene ranks as the most common genetic events in human cancers. A large body of evidence indicates that p53 is required for maintaining the integrity of the genome under environmental stresses. p53 is a transcription factor, central to the action of this tumor suppressor is its ability to activate specific genes carrying distinct p53 binding motif. We have isolated several novel p53 target genes, and are currently studying the expression and functions using cell culture systems as well as mouse model.
Wang, M.C., Liu, J.H., and Wang, F.F. (1997) Protein tyrosine phosphatase dependent activation of b-globin and d-aminolevulinic acid synthase genes in the camptothecin-induced IW32 erytholeukemia cell differentiation. Mol. Pharmcol. 51,558-566.
Chuang, C.C., Tan, S.K., Tai, L.K., Hsin, J.P. and Wang, F.F. (1998) Evidence for the involvement of protein kinase C in the inhibition of prolactin gene expression by transforming growth factor-b2. Mol. Pharmacol. 53, 1054-1061.
Jang, Y.C., Kao, L.S. and Wang, F.F (1998) Involvement of Ca2+ signaling in the vasoactive intestinal peptide and 8-Br-cAMP induction of c-fos mRNA expression. Cell, Signal. 10, 27-34.
Lo, P.K., Chen, J.Y., Lo, W.C., Chen, B.F., Hsin, J.P., Tang, P.P. and Wang, F.F. (1999) Identification of a novel mouse p53 target gene DDA3. Oncogene 18, 7765-7774
Tang, P.P. and Wang, F.F. (2000) Induction of IW32 erythroleukemia cell differentiation by p53 is dependent on protein tyrosine phosphatase. Leukemia 14, 1292-1300.
Lo. P.K., Chen, J.Y., Tang, P.P., Lin, J., Lin, C.H., Su, L.T., Wu, C.H., Chen, T.L., Yang, Y. and Wang, F.F. (2001) Identification of a mouse thiamine transporter gene as a direct transcriptional target for p53. J. Biol. Chem. 276, 37186-37193.
Lo, P.K. and Wang, F.F. (2002) Identification of transcriptional start sites and splicing of mouse thiamine transporter gene, THTR-1. Biochim. Biophys. Acta.1576, 209-213.
Hsien, S.C., Lo, P.K. and Wang, F.F. (2002) DDA3 is a direct transcriptional target gene of p53 and p73. Oncogene 21, 3050-3057.
Tang, P.P., S.C. Hsieh and Wang, F.F. (2002) Modulation of caspase activation and p27Kip1 degradation in the p53 induced apoptosis in IW32 erythroleukemia cells. Cell. Signal. 14, 961-968.
Lo, P.K and Wang, F.F. (2002) Cloning and characterization of human and mouse DDA3 genes. Biochim. Biophys. Acta 1579, 214-218.
Lo, P.K and Wang, F.F. (2004) Heterogeneity of mouse Dda3 transcripts is attributed to differential initiation of transcription and alternative splicing. Arch. Biochem. Biophys. 425, 221-232.
Lo, P.K., Huang, S.Z., Chen, H.C. and Wang, F.F. (2004) The prosurvival activity of p53 protects cells from UV-induced apoptosis by inhibiting JNK activation and mitochondrial death signaling. Cancer Research . 8736-8745
Shu-Chen Hsieh, Fung-Fang Wang, Ching-Shih Lin, Yu-Ju Chen, Shih-Chieh Hung,. (2006) The inhibition of osteogenesis with human bone marrow mesenchymal stem cells by CdSe/ZnS quantum dot labels. BIOMATERIALS , 27:1656-1664.
Hsieh SC, Wang FF, Hung SC, Chen YJ, Wang YJ. (2006) The internalized CdSe/ZnS quantum dots impair the chondrogenesis of bone marrow mesenchymal stem cell.. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS .79, 95-101.
Hsieh PC, Chang JC, Sun WT, Hsieh SC, Wang MC and Wang FF. (2007) p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway. Oncogene (in press).