Chin-hsiang Chien

PhD. Biochemistry, National Taiwan University

Research

  1. Study of tumor supressor Fhit associated protein
    Characterization of Fhit associated protein by yeast-two hybrid, proteomics, confocal  microscope and PET. 
    Application of Fhit associated protein in nude mice as a potential gene for gene therapy.

  2. Growth control, signal transduction and gene therapy of human ovarian cancers
    Cloning of estrogen induced cancer gene by microarray analysis. 
    Signal transduction pathway of TGF induced growth arrest gene of human ovarian cancer. 

  3. Cloning, expression, and structure- functional analysis of sialidase (nanH) 
    Cloning , sequencing and structure analysis of porcine lysosomal sialidase. 
    Application of sialidase gene to study cell-cell adhesions and cancer metastasis.

Publications

  1. Chow, S.N., Lin, J.K., Li, S.L., and Chien, C.H. (1997). Identification of LDH-ras p21 protein complex and expression of these genes in human ovarian cancer. Gynecol. Oncology. 64, 114-120.
  2. Chien, C.H., Chow, S.H. (1997). Detection of serum transforming growthfactor-a in pationts of primary epithelial ovarian cancers by enzyme immunoassay. Gynecol. Oncology. 66, 405-410.
  3. Chang, Y.K., and Chien, C.H. (1998). Simple two-step procedure for purification of cloned small neuraminidase from unclarified Escharichia coli feedstocks. Enz. Microb. Tech. 23, 204-210.    
  4. Chow, S.N., Chen, M., Chien, C.H. (2001). Cell cycle analysis and detection of proliferative cell nuclear antigen of the endometrium after hormone replacement therapy. Maturitas, 39, 227-237.
  5. Sheu, S.Y., Huang, S.P., Tzeng, H.J., Chien, C.H. (2002). Cloning, overexpression and deletion analysis of cloned large nanH from Clostridium perfringens.Enz.Microb.Tech. 31/6, 794-830.
  6. Chen, C.T., Chien, C.H., Chow, S.N. (2003). Expression of CDK2 and loss of expression of p16ink4 in human ovarian cancer..J .of Genetic and Molecular Biology, 14, 24-29.
  7. Chow, S.N., Wang, S., Chien, C.H. (2004). Analysis of chromosomal aberrations by comparative genomic hybridization in a primitive neuroectodermal tumor of the ovary. Gynecol. Oncology. 92, 752-760.
  8. Chien, C.H., Chen, C.H., Lee, G. (2004). Detection of gonadotropin-releasing hormone (GnRH) receptor and it mRNA in primary human epithelial ovarian cancer. Internat. J. Gynecol. Cancer. 14, 451-458.
  9. Newstead, S, Chien, C.H., Taylor, M. Taylor, (2004). Crystallization and atomic resolution X-ray diffraction of the catalytic domain of the large sialidase, nanI, from Clostridium perfringens', Acta Crystallographica, Section D60(1): 2063-2066.
  10. Chin-hsinag Chien Chun-Huang Lin. Patent: Pharmaceutical composition comprising Nap1 for use in inhibiting tumow cell growth. Patent No. 204099 2004;1(1):
  11. Chow, S.N., Chen, C.H., Chien, C.H. (2004). Complementary-DNA microarray approach to analyze estrogen-responsive genes in human ovarian cancer cells, Int J. of Gyn. Cancer, 14 (suppl.1) 236.
  12. Lee, G, Wu, Q, Li, CH, Ting, HH, Chien CH. Recent Studies of a New Carbohydrate-Associated Pan Cancer Marker, CA215. J. of Clinical Ligand Assay 2006;29(1):1-5
  13. Lin, Chun-huang, Chuang, Min-Yi, Chien, Chin-hsuang. Growth inhibitory effect of human NIT2 gene and its allelic imbalance in cancers. FEBS Journal 2007 Apr, 10 accepted