Bon-Chu Chung

PhD. Biochemistry, University of Pennsylvania

Research

    Steroid Function and Regulation in Mouse-- We have established mouse models with Cyp11a1I gene mutation to study steroid deficiency.   

    Steroid Function and Regulation in Zebrafish Development¡XWe have cloned zebrafish steroidogenic genes and are studying their functions during  development using knockdown and overexpression strtegies

    Mechanism of Transcriptional Regulation of Steroidogenic Genes¡XIn this area, we study the proteins that interact with transcription factor SF-1 involved in steroidogenic gene transcription.

Publications

  1. Lin,W.W., Wang, H.W., Sum,C., Liu,D., Hew, C.L., and Chung,B.C. (2000) Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and expressed in digestive organs. Biochem. J. 348, 439-446.
  2. Chiang,E.F.L., Pai, C.I., Wyatt, M., Yan,Y.L, Postlethwait, J., and Chung,B.C. (2001) Two sox9 genes on duplicated zebrafish chromosomes: expression of similar transcription activators in distinct sites. Dev. Biol. 231, 149-163.
  3. Chiang,E.F.L., Tong,S.K., Yan,Y.L., Hsiao,P.H., Guiguen,Y., Postlethwait, J., and Chung, B.C. (2001) Two functional Cyp19 (P450 aromatase) genes on duplicated zebrafish chromosomes are expressed in ovary or brain. Mol. Biol. Evol. 18, 542-550.
  4.  Hu,M.C., Hsu, N.C, Pai,C.I., Wang,C.K.L., and Chung,B.C. (2001) Upstream and proximal steroidogenic factor 1 (SF-1)-binding sites in the cyp11a1 promoter in basal transcription and hormonal response.  Mol. Endocrinol. 15, 812-818.
  5. Hu,M.C., Hsu, N.C, Ben, E.l Hadj N, PaiC.I, Chu ,H.P, Wang, C.K.L, Chung, B.C. (2002) ¡§Steroid Deficiency Syndromes in Mice with a Targeted Disruption of Cyp11a1¡¨ Mol. Endocrinol. 16, 1943-1950.
  6. Yan,Y.L, Miller,C.T., Nissen,R.M., Singer,A., Liu,D., Kirt,A., Draper,B., Willoughby,J., Morcos,P.A., Chung, B.C., Westerfield M, Haffter P, Hopkins N, Kimmel C, Postlethwait, J.H. (2002) A zebrafish sox9 gene required for cartilage morphogenesis. Development, 129, 5060-5079.
  7. Hsu,H.J., Hsiao,P, Kuo, M., Chung,B.C. (2002) Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer. Gene Expr. Patterns 2, 219-222.
  8. Hsu,H.J., Lin G, Chung,B.C. (2003) Parallel Early Development of Zebrafish Interrenal and Pronephro: Differential Control by wt1 and ff1b. Development, 130, 2107-2116.
  9. Chen,W.Y., Lee,W.C., Hsu, N.C., Huang,F., and Chung,B.C. (2004) SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1).  J. Biol. Chem. 279, 38730-38735.
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